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Cell No. : Cell Name
RCB2113 : KUM6  update : 2023/10/16
CommentMouse mesenchymal cell line derived from bone marrow. C3H/He
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal _mouse < Mammals
Strain name C3H/He
Gender Female
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5152
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2-3 x 10 5 cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Achoreplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 0
User's Publication 2


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Reference

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User's Publication
12052  Fujita K, Kuge K, Ozawa N, Sahara S, Zaiki K, Nakaoji K, Hamada K, Takenaka Y, Tanahashi T, Tamai K, Kaneda Y, Maeda A.  Cinnamtannin B-1 Promotes Migration of Mesenchymal Stem Cells and Accelerates Wound Healing in Mice  PLoS One  2015  10(12):e0144166  PubMed ID: 26657737   DOI: 10.1371/journal.pone.0144166
17151  Furumoto T, Ozawa N, Inami Y, Toyoshima M, Fujita K, Zaiki K, Sahara S, Akita M, Kitamura K, Nakaoji K, Hamada K, Tamai K, Kaneda Y, Maeda A.  Mallotus philippinensis bark extracts promote preferential migration of mesenchymal stem cells and improve wound healing in mice  Phytomedicine  2014  21(3):247-53  PubMed ID: 24182990   DOI: 10.1016/j.phymed.2013.09.003



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