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Cell No. : Cell Name
RCB2112 : KUM4  update : 2021/05/20
CommentMouse mesenchymal cell line derived from bone marrow. C3H/He
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal mouse < Mammals
Strain name C3H/He
Gender Female
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5150
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2-3 x 10^(5) cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
2753  Umezawa A, Maruyama T, Segawa K, Shadduck RK, Waheed A, Hata J  Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.  J Cell Physiol  1992  151:197-205  PubMed ID: 1373147

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User's Publication
8493  Kurtova AV, Balakrishnan K, Chen R, Ding W, Schnabl S, Quiroga MP, Sivina M, Wierda WG, Estrov Z, Keating MJ, Shehata M, Jager U, Gandhi V, Kay NE, Plunkett W, Burger JA.  Diverse marrow stromal cells protect CLL cells from spontaneous and drug-induced apoptosis: development of a reliable and reproducible system to assess stromal cell adhesion-mediated drug resistance.  Blood  2009  114:4441-50  PubMed ID: 19762485



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