Cell No. : Cell Name
RCB2111 : K562/Vin
update : 2024/05/30
|
| Comment | Subline of K562 cell line. Resistant to vincristine. TKG0212 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Obinata, Masuo
|
| Originator |
Tsuruo, T.
|
| Year of deposit |
2005
|
| Original cell |
TKG0212
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
53 years
|
| Tissue |
pleural effusion
|
| Disease name |
chrinic myelogenous leukemia
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0212）
|
|
Lifespan |
infinite
|
| Morphology |
lymphoblast-like
|
| Cellosaurus(Expasy) |
CVCL_5145
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
LT 400
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
|
| User's Publication |
2
|
| Reference |
|
5099
Tsuruo T, Iida H, Ohkochi E, Tsukagoshi S, Sakurai Y.
Establishment and properties of vincristine-resistant human myelogenous leukemia K562.
Gan
1983
74(5):751-8
PubMed ID: 6580255
|
|
87
Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.
A multipotential leukemia cell line (K-562) of human origin
Proc Soc Exp Biol Med
1981
166(4):546-50
PubMed ID: 7194480
DOI: 10.3181/00379727-166-41106
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|
4603
Lozzio BB, Lozzio CB.
Properties and usefulness of the original K-562 human myelogenous leukemia cell line.
Leuk Res
1979
3(6):363-70
PubMed ID: 95026
DOI: 10.1016/0145-2126(79)90033-x
|
|
969
Rutherford TR, Clegg JB, Weatherall DJ.
K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
Nature
1979
280(5718):164-5
PubMed ID: 95354
DOI: 10.1038/280164a0
|
|
970
Andersson LC, Jokinen M, Gahmberg CG.
Induction of erythroid differentiation in the human leukaemia cell line K562.
Nature
1979
278(5702):364-5
PubMed ID: 570644
DOI: 10.1038/278364a0
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|
4602
Lozzio CB, Lozzio BB.
Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
Blood
1975
45(3):321-34
PubMed ID: 163658
|
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