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Cell No. : Cell Name
RCB2110 : YKG1  update : 2021/05/20
CommentHuman cell line derived from glioblastoma. TKG0453 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Acta Neuropathologica, 79, 30-36, 1989) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kanno, Hiroshi
Year of deposit 2005
Original cell TKG0453
Animal human < Mammals
Genus Homo
Species sapiens
Tissue brain
Disease name Glioblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0453)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1796
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
5098  Kanno H, Kuwabara T, Shinonaga M, Chang CC, Tanaka Y, Sugio Y, Morita H, Yasumitsu H, Umeda M, Nagashima Y.  Establishment of a human glioma cell line bearing a homogeneously staining chromosomal region and releasing alpha- and beta-type transforming growth factors.  Acta Neuropathol  1989  79(1):30-6  PubMed ID: 2589023   DOI: 10.1007/bf00308954

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User's Publication
4375  Fukushima T, Kawaguchi M, Yamamoto K, Yamashita F, Izumi A, Kaieda T, Takezaki Y, Itoh H, Takeshima H, Kataoka H.  Aberrant methylation and silencing of the SPINT2 gene in high-grade gliomas.  Cancer Sci.  2018  109(9):2970-2979  PubMed ID: 29987920   DOI: 10.1111/cas.13732
14212  GursesCila HE, Acar M, Barut FB, Gunduz M, Grenman R, Gunduz E.  Investigation of the expression of RIF1 gene on head and neck, pancreatic and brain cancer and cancer stem cells  Clin Invest Med  2016  39(6):27500  PubMed ID: 27917791  
16012  Ohashi R, Matsuda Y, Ishiwata T, Naito Z.  Downregulation of fibroblast growth factor receptor 2 and its isoforms correlates with a high proliferation rate and poor prognosis in high-grade glioma  Oncol Rep  2014  32(3):1163-9  PubMed ID: 24968791   DOI: 10.3892/or.2014.3283
13710  Fukushima T, Kawaguchi M, Yorita K, Tanaka H, Takeshima H, Umezawa K, Kataoka H.  Antitumor effect of dehydroxymethylepoxyquinomicin, a small molecule inhibitor of nuclear factor-κB, on glioblastoma  Neuro Oncol  2012  14(1):19-28  PubMed ID: 21968049   DOI: 10.1093/neuonc/nor168
3698  Fukushima T, Tezuka T, Shimomura T, Nakano S, Kataoka H.  Silencing of insulin-like growth factor-binding protein-2 in human glioblastoma cells reduces both invasiveness and expression of progression-associated gene CD24.  J Biol Chem  2007  282(25):18634-44  PubMed ID: 17475624   DOI: 10.1074/jbc.M609567200



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