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Cell No. : Cell Name
RCB2108 : NH-12  update : 2021/05/20
CommentHuman cell line derived from neuroblastoma. TKG0448 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Hiyama, E. & Nakamura, N.
Year of deposit 2005
Original cell TKG0448
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue neuroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0448)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1605
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 2

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5096  Hiyama E, Hiyama K, Yokoyama T, Ichikawa T, Matsuura Y.  Length of telomeric repeats in neuroblastoma: correlation with prognosis and other biological characteristics.  Jpn J Cancer Res  1992  83(2):159-64  PubMed ID: 1555997

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User's Publication
13399  Dileep KV, Sakai N, Ihara K, Kato-Murayama M, Nakata A, Ito A, Sivaraman DM, Shin JW, Yoshida M, Shirouzu M, Zhang KYJ.  Piperidine-4-carboxamide as a new scaffold for designing secretory glutaminyl cyclase inhibitors.  Int J Biol Macromol  2021  170:415-423.  PubMed ID: 33373636
10389  Fukuhara S, Nomoto J, Kim SW, Taniguchi H, Miyagi Maeshima A, Tobinai K, Kobayashi Y.  Partial deletion of the ALK gene in ALK-positive anaplastic large cell lymphoma.  Hematol Oncol  2018  36:150-158  PubMed ID: 28665006

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