Cell No. : Cell Name
RCB2107 : TN-1 update : 2023/10/16 |
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Comment | Human cell line derived from neuroblastoma formed at adrenal gland. TKG0277 (Deposited from Tohoku Univ.). | |||
Comment from the depositor | ||||
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
Remarks | ||||
Order Form | Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Obinata, Masuo | ||
Originator | Hayashi, T. (Pediat. Surg., Tohoku Univ.). | |||
Year of deposit | 2005 | |||
Original cell | TKG0277 | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Gender | Male | |||
Age at sampling | 2 years | |||
Tissue | right adrenal gland | |||
Classification | cancer | |||
History | Cell Resource Center for Biomedical Research, Tohoku University(TKG277) | Lifespan | infinite | |
Morphology | epithelial-like | |||
Cellosaurus(Expasy) | CVCL_6738 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | RPMI1640 + 10% FBS | |||
Antibiotics | Free | Passage method | pipetting | Culture information | Passage ratio | 1 : 4 split |
SC frequency | Subculture : once/3 weeks, Medium Renewal : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Isozyme | LD, NP | |||
STR(human) | OK | |||
Images | ||||
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Reference information | Reference | 1 | ||
User's Publication | 2 | |||
Reference |
5095 Yaoita S1, Ohoi R, Hayashi Y, Watanabe T. Establishment and characterization of TN-1 cell line from a neuroblastoma of the right adrenal gland Hum Cell 1989 2(2):198 PubMed ID: 2486611 |