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Cell No. : Cell Name
RCB2107 : TN-1  update : 2022/04/15
CommentHuman cell line derived from neuroblastoma formed at adrenal gland. TKG0277 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Hayashi, T. (Pediat. Surg., Tohoku Univ.).
Year of deposit 2005
Original cell TKG0277
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 2 years
Tissue right adrenal gland
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG277)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_6738
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/3 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5095  Yaoita S1, Ohoi R, Hayashi Y, Watanabe T.  Establishment and characterization of TN-1 cell line from a neuroblastoma of the right adrenal gland  Hum Cell  1989  2(2):198  PubMed ID: 2486611  

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