Cell No. : Cell Name RCB2107 : TN-1  update : 2023/10/16
Comment			Human cell line derived from neuroblastoma formed at adrenal gland. TKG0277 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Hayashi, T. (Pediat. Surg., Tohoku Univ.).
		Year of deposit	2005
		Original cell	TKG0277
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	2 years
		Tissue	right adrenal gland
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG277）
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_6738
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		pipetting
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/3 weeks, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

5095  Yaoita S1, Ohoi R, Hayashi Y, Watanabe T.  Establishment and characterization of TN-1 cell line from a neuroblastoma of the right adrenal gland  Hum Cell  1989  2(2):198  PubMed ID: 2486611

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User's Publication

18785  Sugito K, Kawashima H, Yoshizawa S, Uekusa S, Hoshi R, Furuya T, Kaneda H, Hosoda T, Konuma N, Masuko T, Ohashi K, Ikeda T, Koshinaga T, Tomita R, Shinojima Y, Fujiwara K, Watanabe T, Held WA, Nagase H.  Non-promoter DNA hypermethylation of Zygote Arrest 1 (ZAR1) in neuroblastomas  J Pediatr Surg  2013  48(4):782-8  PubMed ID: 23583134   DOI: 10.1016/j.jpedsurg.2012.08.008

18782  Kawashima H, Sugito K, Yoshizawa S, Uekusa S, Furuya T, Ikeda T, Koshinaga T, Shinojima Y, Hasegawa R, Mishra R, Igarashi J, Kimura M, Wang X, Fujiwara K, Gosh S, Nagase H.  DNA hypomethylation at the ZNF206-exon 5 CpG island associated with neuronal differentiation in mice and development of neuroblastoma in humans  Int J Oncol  2012  40(1):31-9  PubMed ID: 22011711   DOI: 10.3892/ijo.2011.1234