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Cell No. : Cell Name
RCB2106 : ME-180  update : 2021/05/20
CommentHuman cell line derived from cervical cancer. TKG0437 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Sykes, J. A.
Year of deposit 2005
Original cell TKG0437
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 66 years
Tissue cervix
Disease name Omental metastasis of rapidly spreading carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0437)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1401
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 4


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Reference
5093  Fitzgerald TJ, Miller JN, Sykes JA.  Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.  Infect Immun  1975  11(5):1133-40  PubMed ID: 1091562   DOI: 10.1128/IAI.11.5.1133-1140.1975
5094  Fitzgerald TJ, Miller JN, Sykes JA.  Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.  Infect Immun  1975  11(5):1141-6  PubMed ID: 804444   DOI: 10.1128/IAI.11.5.1141-1146.1975
5092  Sykes JA, Whitescarver J, Jernstrom P, Nolan JF, Byatt P.  Some properties of a new epithelial cell line of human origin.  J Natl Cancer Inst  1970  45(1):107-22  PubMed ID: 4317734  

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User's Publication
20389  D S P, Chaturvedi PK, Shimokawa T, Kim KH, Park WY.  Silencing of Fused Toes Homolog (FTS) Increases Radiosensitivity to Carbon-Ion Through Downregulation of Notch Signaling in Cervical Cancer Cells.  Front Oncol  2021  11:730607  PubMed ID: 34765546   DOI: 10.3389/fonc.2021.730607
5011  Nagura M, Matsumura N, Baba T, Murakami R, Kharma B, Hamanishi J, Yamaguchi K, Abiko K, Koshiyama M, Mandai M, Murata T, Murphy SK, Konishi I.  Invasion of uterine cervical squamous cell carcinoma cells is facilitated by locoregional interaction with cancer-associated fibroblasts via activating transforming growth factor-beta.  Gynecol Oncol  2015  136(1):104-11  PubMed ID: 25434636   DOI: 10.1016/j.ygyno.2014.11.075
16150  Imadome K, Iwakawa M, Nakawatari M, Fujita H, Kato S, Ohno T, Nakamura E, Ohkubo Y, Tamaki T, Kiyohara H, Imai T.  Subtypes of cervical adenosquamous carcinomas classified by EpCAM expression related to radiosensitivity  Cancer Biol Ther  2010  10(10):1019-26  PubMed ID: 20855955   DOI: 10.4161/cbt.10.10.13249
5012  Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K.  Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma.  Hum Mol Genet  2006  15(6):821-30  PubMed ID: 16439445   DOI: 10.1093/hmg/ddl001



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