Cell No. : Cell Name
RCB2106 : ME-180
update : 2024/08/28
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| Comment | Human cell line derived from cervical cancer. TKG0437 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Obinata, Masuo
|
| Originator |
Sykes, J. A.
|
| Year of deposit |
2005
|
| Original cell |
TKG0437
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Caucasian
|
| Gender |
Female
|
| Age at sampling |
66 years
|
| Tissue |
cervix
|
| Disease name |
Omental metastasis of rapidly spreading carcinoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0437）
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_1401
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
6
|
| Reference |
|
5093
Fitzgerald TJ, Miller JN, Sykes JA.
Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
Infect Immun
1975
11(5):1133-40
PubMed ID: 1091562
DOI: 10.1128/IAI.11.5.1133-1140.1975
|
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5094
Fitzgerald TJ, Miller JN, Sykes JA.
Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
Infect Immun
1975
11(5):1141-6
PubMed ID: 804444
DOI: 10.1128/IAI.11.5.1141-1146.1975
|
|
5092
Sykes JA, Whitescarver J, Jernstrom P, Nolan JF, Byatt P.
Some properties of a new epithelial cell line of human origin.
J Natl Cancer Inst
1970
45(1):107-22
PubMed ID: 4317734
|
| User's Publication |
|
18210
Krishnamurthy S, Yoda H, Hiraoka K, Inoue T, Lin J, Shinozaki Y, Watanabe T, Koshikawa N, Takatori A, Nagase H.
Targeting the mutant PIK3CA gene by DNA-alkylating pyrrole-imidazole polyamide in cervical cancer
Cancer Sci
2021
112(3):1141-1149
PubMed ID: 33377228
DOI: 10.1111/cas.14785
|
|
20389
D S P, Chaturvedi PK, Shimokawa T, Kim KH, Park WY.
Silencing of Fused Toes Homolog (FTS) Increases Radiosensitivity to Carbon-Ion Through Downregulation of Notch Signaling in Cervical Cancer Cells.
Front Oncol
2021
11:730607
PubMed ID: 34765546
DOI: 10.3389/fonc.2021.730607
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5011
Nagura M, Matsumura N, Baba T, Murakami R, Kharma B, Hamanishi J, Yamaguchi K, Abiko K, Koshiyama M, Mandai M, Murata T, Murphy SK, Konishi I.
Invasion of uterine cervical squamous cell carcinoma cells is facilitated by locoregional interaction with cancer-associated fibroblasts via activating transforming growth factor-beta.
Gynecol Oncol
2015
136(1):104-11
PubMed ID: 25434636
DOI: 10.1016/j.ygyno.2014.11.075
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19220
Nakamura E, Satoh T, Iwakawa M, Nakawatari M, Oki A, Matsumoto K, Okada S, Minaguchi T, Yoshikawa H, Imai T.
Villin1, a diagnostic marker for endometrial adenocarcinoma with high grade nuclear atypia
Cancer Biol Ther
2011
12(3):181-90
PubMed ID: 21623163
DOI: 10.4161/cbt.12.3.15964
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16150
Imadome K, Iwakawa M, Nakawatari M, Fujita H, Kato S, Ohno T, Nakamura E, Ohkubo Y, Tamaki T, Kiyohara H, Imai T.
Subtypes of cervical adenosquamous carcinomas classified by EpCAM expression related to radiosensitivity
Cancer Biol Ther
2010
10(10):1019-26
PubMed ID: 20855955
DOI: 10.4161/cbt.10.10.13249
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5012
Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K.
Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma.
Hum Mol Genet
2006
15(6):821-30
PubMed ID: 16439445
DOI: 10.1093/hmg/ddl001
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