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Cell No. : Cell Name
RCB2105 : D98-AH2  update : 2021/05/20
CommentHuman cell line derived from cervical cancer. The cells were converted to HGPRT(-). A subline of HeLa cell. TKG0191 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Matsuya, Y.
Year of deposit 2005
Original cell TKG0191
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0191)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8190
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 6
User's Publication 1


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Reference
3482  Srivatsan ES, Bengtsson U, Manickam P, Benyamini P, Chandrasekharappa SC, Sun C, Stanbridge EJ, Redpath JL.  Interstitial deletion of 11q13 sequences in HeLa cells.  Genes Chromosomes Cancer  2000  29(2):157-65  PubMed ID: 10959095   DOI: 10.1002/1098-2264(2000)9999:9999<::aid-gcc1024>3.0.co;2-p
3481  Ruess D, Ye LZ, Grond-Ginsbach C.  HeLa D98/aH-2 studied by chromosome painting and conventional cytogenetical techniques.  Chromosoma  1993  102(7):473-7  PubMed ID: 7690695   DOI: 10.1007/bf00357102
3480  Pereira-Smith OM1, Stein GH, Robetorye S, Meyer-Demarest S.  Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts.  J Cell Physiol  1990  143(2):222-5  PubMed ID: 2332448   DOI: 10.1002/jcp.1041430204
3479  Matsuya Y, Green H.  Somatic cell hybrid between the established human line D98 (presumptive HeLa) and 3T3.  Science  1969  163(3868):697-8  PubMed ID: 5762936   DOI: 10.1126/science.163.3868.697
5090  SZYBALSKA EH, SZYBALSKI W.  Genetics of human cess line. IV. DNA-mediated heritable transformation of a biochemical trait.  Proc Natl Acad Sci U S A  1962  48:2026-34  PubMed ID: 13980043  
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
5012  Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K.  Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma.  Hum Mol Genet  2006  15(6):821-30  PubMed ID: 16439445   DOI: 10.1093/hmg/ddl001



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