Cell No. : Cell Name
RCB2105 : D98-AH2
update : 2023/10/16
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| Comment | Human cell line derived from cervical cancer. The cells were converted to HGPRT(-). A subline of HeLa cell. TKG0191 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Obinata, Masuo
|
| Originator |
Matsuya, Y.
|
| Year of deposit |
2005
|
| Original cell |
TKG0191
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0191）
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8190
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Isozyme |
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LD, NP
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| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
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| User's Publication |
1
|
| Reference |
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3482
Srivatsan ES, Bengtsson U, Manickam P, Benyamini P, Chandrasekharappa SC, Sun C, Stanbridge EJ, Redpath JL.
Interstitial deletion of 11q13 sequences in HeLa cells.
Genes Chromosomes Cancer
2000
29(2):157-65
PubMed ID: 10959095
DOI: 10.1002/1098-2264(2000)9999:9999<::aid-gcc1024>3.0.co;2-p
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3481
Ruess D, Ye LZ, Grond-Ginsbach C.
HeLa D98/aH-2 studied by chromosome painting and conventional cytogenetical techniques.
Chromosoma
1993
102(7):473-7
PubMed ID: 7690695
DOI: 10.1007/bf00357102
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3480
Pereira-Smith OM1, Stein GH, Robetorye S, Meyer-Demarest S.
Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts.
J Cell Physiol
1990
143(2):222-5
PubMed ID: 2332448
DOI: 10.1002/jcp.1041430204
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3479
Matsuya Y, Green H.
Somatic cell hybrid between the established human line D98 (presumptive HeLa) and 3T3.
Science
1969
163(3868):697-8
PubMed ID: 5762936
DOI: 10.1126/science.163.3868.697
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5090
SZYBALSKA EH, SZYBALSKI W.
Genetics of human cess line. IV. DNA-mediated heritable transformation of a biochemical trait.
Proc Natl Acad Sci U S A
1962
48:2026-34
PubMed ID: 13980043
DOI: 10.1073/pnas.48.12.2026
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150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
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