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Cell No. : Cell Name
RCB2104 : HuO-3N1  update : 2021/04/08
CommentHuman cell line derived from osteosarcoma. TKG0452 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Yamane, T. & Sato, J
Year of deposit 2005
Original cell TKG0451
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 15 years
Tissue osteosarcoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0452)
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 7

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5089  Yamane T.  Establishment and characterization of cell lines derived from a human osteosarcoma.  Clin Orthop Relat Res  1985  (199):261-71  PubMed ID: 3862491

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User's Publication
13409  Hirozane T, Masuda M, Sugano T, Sekita T, Goto N, Aoyama T, Sakagami T, Uno Y, Moriyama H, Sawa M, Asano N, Nakamura M, Matsumoto M, Nakayama R, Kondo T, Kawai A, Kobayashi E, Yamada T.  Direct conversion of osteosarcoma to adipocytes by targeting TNIK.  JCI Insight  2021    PubMed ID: 33400690
13419  Suzuki S, Yuan H, Hirata-Tsuchiya S, Yoshida K, Sato A, Nemoto E, Shiba H, Yamada S.  DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.  Mol Cell Biochem  2021    PubMed ID: 33420898
6403  Terasaka N, Futai K, Katoh T, Suga H.  A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX.  RNA  2016  22(12):1918-1928  PubMed ID: 27852928
7403  Takagi M, Tokunaga N.  Noninvasive discrimination between human normal and cancer cells by analysis of intracellular distribution of phase-shift data.  Cytotechnology  2015  67:733-9  PubMed ID: 25854623
6427  Kobayashi E, Satow R, Ono M, Masuda M, Honda K, Sakuma T, Kawai A, Morioka H, Toyama Y, Yamada T.  MicroRNA expression and functional profiles of osteosarcoma.  Oncology  2014  86(2):94-103  PubMed ID: 24457375
6431  Shibuya H, Hamamura K, Hotta H, Matsumoto Y, Nishida Y, Hattori H, Furukawa K, Ueda M, Furukawa K.  Enhancement of malignant properties of human osteosarcoma cells with disialyl gangliosides GD2/GD3.  Cancer Sci  2012  103(9):1656-64  PubMed ID: 22632091
6435  Yamaguchi U, Honda K, Satow R, Kobayashi E, Nakayama R, Ichikawa H, Shoji A, Shitashige M, Masuda M, Kawai A, Chuman H, Iwamoto Y, Hirohashi S, Yamada T.  Functional genome screen for therapeutic targets of osteosarcoma.  Cancer Sci  2009  100(12):2268-74  PubMed ID: 19725836

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