Cell No. : Cell Name
RCB2104 : HuO-3N1
update : 2021/01/08
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| Comment | Human cell line derived from osteosarcoma. TKG0452 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Obinata, Masuo
|
| Originator |
Yamane, T. & Sato, J
|
| Year of deposit |
2005
|
| Original cell |
TKG0451
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
15 years
|
| Tissue |
osteosarcoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0452）
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|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Medium and additives |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
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0.25% trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
|
| STR(human) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
7
|
| User's Publication |
|
13409
Hirozane T, Masuda M, Sugano T, Sekita T, Goto N, Aoyama T, Sakagami T, Uno Y, Moriyama H, Sawa M, Asano N, Nakamura M, Matsumoto M, Nakayama R, Kondo T, Kawai A, Kobayashi E, Yamada T.
Direct conversion of osteosarcoma to adipocytes by targeting TNIK.
JCI Insight
2021
PubMed ID: 33400690
|
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13419
Suzuki S, Yuan H, Hirata-Tsuchiya S, Yoshida K, Sato A, Nemoto E, Shiba H, Yamada S.
DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.
Mol Cell Biochem
2021
PubMed ID: 33420898
|
|
6403
Terasaka N, Futai K, Katoh T, Suga H.
A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX.
RNA
2016
22(12):1918-1928
PubMed ID: 27852928
|
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7403
Takagi M, Tokunaga N.
Noninvasive discrimination between human normal and cancer cells by analysis of intracellular distribution of phase-shift data.
Cytotechnology
2015
67:733-9
PubMed ID: 25854623
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6427
Kobayashi E, Satow R, Ono M, Masuda M, Honda K, Sakuma T, Kawai A, Morioka H, Toyama Y, Yamada T.
MicroRNA expression and functional profiles of osteosarcoma.
Oncology
2014
86(2):94-103
PubMed ID: 24457375
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6431
Shibuya H, Hamamura K, Hotta H, Matsumoto Y, Nishida Y, Hattori H, Furukawa K, Ueda M, Furukawa K.
Enhancement of malignant properties of human osteosarcoma cells with disialyl gangliosides GD2/GD3.
Cancer Sci
2012
103(9):1656-64
PubMed ID: 22632091
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6435
Yamaguchi U, Honda K, Satow R, Kobayashi E, Nakayama R, Ichikawa H, Shoji A, Shitashige M, Masuda M, Kawai A, Chuman H, Iwamoto Y, Hirohashi S, Yamada T.
Functional genome screen for therapeutic targets of osteosarcoma.
Cancer Sci
2009
100(12):2268-74
PubMed ID: 19725836
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