| Cell No. : Cell Name RCB2103 : 8505C
						
						 update : 2023/10/16
 | 
						
						
						
							
								| Comment | Human cell line derived from thyroid cancer. Poorly differentiated papillary adenocarcinoma.  TKG0439   (Deposited from Tohoku Univ.). | 
							
								| Comment from the depositor |  | 
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
								| Remarks |  | 
							
							
								| Order Form | Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										  | 
							
								| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | 
						
						
					
						
						
							
							|  | Basic information | Depositor | Obinata, Masuo | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Originator | Nakamura, N. | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of deposit | 2005 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Original cell | TKG0439 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Animal | _human
								
								
								
							
								 
								
								
								 	
								 		< Mammals | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Genus | Homo | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Species | sapiens | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Tissue | thyroid gland | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Disease name | Poorly differentiated papillary adenocarcinoma | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Classification | cancer | 
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | History | Cell Resource Center for Biomedical Research, Tohoku University(TKG0439) | 
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							 | Lifespan | infinite | 
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | epithelial-like | 
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Cellosaurus(Expasy) | CVCL_1054 | 
					
									
								
							
						
					
					
					
					
					
					
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
					
						
						
							
							
								|  |  | deposit info | lot info | 
											
												|  | Medium | Medium List | 
										
									
								
						
						
						
						
						
					
						
						
						
						
							 | Culture type |  | Adherent cells | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Culture medium |  | MEM + 10% FBS | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Antibiotics |  | Free | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							 | Passage method |  | 0.25% Trypsin | Culture information | Passage ratio |  | 1 : 8 split | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| SC frequency |  | Subculture : 2 times/week | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Temperature |  | 37
							
							
						
						
							℃ | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| CO2 concentration |  | 5
							
							
						
						
							% | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freeze medium |  | Medium + 10% DMSO | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freezing method |  | Slow freezing | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Mycoplasma/Acholeplasma |  | (-) | 
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Isozyme |  | LD, NP | 
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| STR(human) |  | OK | 
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
						
							
							
							
								| Images | deposit info | lot info | 
							
							
							|  |  | 
					
			
					
			
						
						
						
							
								| Reference information | Reference | 2 | 
							
								| User's Publication | 9 | 
						
						
				
			
				
				
				
				
				
					
						| Reference | 
						
							
							| 5037 
								Ito T, Seyama T, Hayashi Y, Hayashi T, Dohi K, Mizuno T, Iwamoto K, Tsuyama N, Nakamura N, Akiyama M. 
								Establishment of 2 human thyroid-carcinoma cell-lines (8305c, 8505c) bearing p53 gene-mutations. 
								Int J Oncol 
								1994 
								4(3):583-6 
								
									
										PubMed ID: 21566963
									
								 
								
									
										DOI: 10.3892/ijo.4.3.583 | 
						
							
							| 829 
								Ito T, Seyama T, Iwamoto KS, Hayashi T, Mizuno T, Tsuyama N, Dohi K, Nakamura N, Akiyama M.  
								In vitro irradiation is able to cause RET oncogene rearrangement 
								Cancer Res 
								1993 
								53(13):2940-3 
								
									
										PubMed ID: 8319199 | 
						
					
				
					
						| User's Publication | 
						
							
							| 21432 
								Inoue M, Iizuka Y, Nakamura K, Sato GE, Mizowaki T. 
								Role of albumin Cys34 redox state in the progression of differentiated thyroid carcinoma and induction of ferroptosis. 
								Free Radic Biol Med 
								2023 
								 
								
									
										PubMed ID: 37806598
									
								 
								
									
										DOI: 10.1016/j.freeradbiomed.2023.10.015 | 
						
							
							| 16175 
								Bonaldi E, Gargiuli C, De Cecco L, Micali A, Rizzetti MG, Greco A, Borrello MG, Minna E. 
								BRAF Inhibitors Induce Feedback Activation of RAS Pathway in Thyroid Cancer Cells 
								Int J Mol Sci 
								2021 
								22(11):5744 
								
									
										PubMed ID: 34072194
									
								 
								
									
										DOI: 10.3390/ijms22115744 | 
						
							
							| 20058 
								Aoyama M, Takizawa H, Otani T, Inoue S, Kawakita N, Tsuboi M, Bando Y, Uehara H, Kondo K, Tangoku A. 
								Non‑invasive monitoring of paclitaxel and lenvatinib efficacy against anaplastic thyroid cancer in orthotopic SCID mouse models using small‑animal FDG‑PET/CT. 
								Oncol Rep 
								2020 
								44(4):1709-1716 
								
									
										PubMed ID: 32945510
									
								 
								
									
										DOI: 10.3892/or.2020.7720 | 
						
							
							| 18204 
								Ooizumi Y, Katoh H, Yokota M, Watanabe M, Yamashita K. 
								Epigenetic silencing of HOPX is critically involved in aggressive phenotypes and patient prognosis in papillary thyroid cancer 
								Oncotarget 
								2019 
								10(57):5906-5918 
								
									
										PubMed ID: 31666923
									
								 
								
									
										DOI: 10.18632/oncotarget.27187 | 
						
							
							| 11296 
								Itai N, Shimazu T, Kimura T, Ibe I, Yamashita R, Kaburagi Y, Dohi T, Tonozuka T, Takao T, Nishikawa A. 
								The phosphorylation of sorting nexin 5 at serine 226 regulates retrograde transport and macropinocytosis. 
								PLoS ONE 
								2018 
								 
								
									
										PubMed ID: 30419003
									
								 
								
									
										DOI: 10.1371/journal.pone.0207205 | 
						
							
							| 4466 
								Shimamura M, Yamamoto K, Kurashige T, Nagayama Y. 
								Intracellular redox status controls spherogenicity, an in vitro cancer stem cell marker, in thyroid cancer cell lines. 
								Exp. Cell Res. 
								2018 
								370(2):699-707 
								
									
										PubMed ID: 30053445
									
								 
								
									
										DOI: 10.1016/j.yexcr.2018.07.036 | 
						
							
							| 12914 
								Nambu J, Kobayashi T, Hashimoto M, Tashiro H, Sugino K, Shimamoto F, Kikuchi A, Ohdan H. 
								h-prune affects anaplastic thyroid cancer invasion and metastasis 
								Oncol Rep 
								2016 
								35(6):3445-52 
								
									
										PubMed ID: 27109060
									
								 
								
									
										DOI: 10.3892/or.2016.4759 | 
						
							
							| 19233 
								Shimamura M, Nagayama Y, Matsuse M, Yamashita S, Mitsutake N. 
								Analysis of multiple markers for cancer stem-like cells in human thyroid carcinoma cell lines 
								Endocr J 
								2014 
								61(5):481-90 
								
									
										PubMed ID: 24531915
									
								 
								
									
										DOI: 10.1507/endocrj.ej13-0526 | 
						
							
							| 7971 
								Sato A, Kudo C, Yamakoshi H, Uehara Y, Ohori H, Ishioka C, Iwabuchi Y, Shibata H. 
								Curcumin analog GO-Y030 is a novel inhibitor of IKKβ that suppresses NF-κB signaling and induces apoptosis. 
								Cancer Sci. 
								2011 
								102:1045-51 
								
									
										PubMed ID: 21272158
									
								 
								
									
										DOI: 10.1111/j.1349-7006.2011.01886.x |