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Cell No. : Cell Name
RCB2103 : 8505C  update : 2020/09/24
CommentHuman cell line derived from thyroid cancer. Poorly differentiated papillary adenocarcinoma. TKG0439 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Nakamura, N.
Year of deposit 2005
Original cell TKG0439
Animal human < Mammals
Genus Homo
Species sapiens
Tissue thyroid gland
Case history Poorly differentiated papillary adenocarcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0439)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
5037  to T, Seyama T, Hayashi Y, Hayashi T, Dohi K, Mizuno T, Iwamoto K, Tsuyama N, Nakamura N, Akiyama M.  Establishment of 2 human thyroid-carcinoma cell-lines (8305c, 8505c) bearing p53 gene-mutations.  Int J Oncol  1994  4(3):583-6  PubMed ID: 21566963

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User's Publication
11296  Itai N, Shimazu T, Kimura T, Ibe I, Yamashita R, Kaburagi Y, Dohi T, Tonozuka T, Takao T, Nishikawa A.  The phosphorylation of sorting nexin 5 at serine 226 regulates retrograde transport and macropinocytosis.  PLoS ONE  2018    PubMed ID: 30419003
4466  Shimamura M, Yamamoto K, Kurashige T, Nagayama Y.  Intracellular redox status controls spherogenicity, an in vitro cancer stem cell marker, in thyroid cancer cell lines.  Exp. Cell Res.  2018  370(2):699-707  PubMed ID: 30053445
7971  Sato A, Kudo C, Yamakoshi H, Uehara Y, Ohori H, Ishioka C, Iwabuchi Y, Shibata H.  Curcumin analog GO-Y030 is a novel inhibitor of IKKβ that suppresses NF-κB signaling and induces apoptosis.  Cancer Sci.  2011  102:1045-51  PubMed ID: 21272158



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