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Cell No. : Cell Name
RCB2102 : HO-1-u-1  update : 2021/02/26
CommentHuman cell line derived from squamous cell carcinoma at mouth floor. TKG0455 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Miyauchi, T.
Year of deposit 2005
Original cell TKG0455
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue mouth floor
Disease name Squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0455)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 44

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5088  Sugiyama A, Ishiguro M, Yasue T, Watanabe S, Sugishita N, Yokoyama H, Uno T, Watanabe H, Matsuno Y, Sasaoka I.  An autopsy case of triple cancer associated with superior vena cava syndrome  Gan No Rinsho  1985  31(10):1347-52  PubMed ID: 2997498

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User's Publication
20020  Shishido K, Kuroishi T, Sugawara S.  P2 purinergic receptor signaling and interleukin-1 synergistically induce interleukin-6 production in a human oral squamous carcinoma cell line.  J Oral Biosci  2021    PubMed ID: 33497843
20085  Kase Y, Uzawa K, Wagai S, Yoshimura S, Yamamoto JI, Toeda Y, Okubo M, Eizuka K, Ando T, Nobuchi T, Kawasaki K, Saito T, Iyoda M, Nakashima D, Kasamatsu A, Tanzawa H.  Engineered exosomes delivering specific tumor-suppressive RNAi attenuate oral cancer progression.  Sci Rep  2021  11(1):5897  PubMed ID: 33723306
11354  Nakamura Y, Nakahata S, Kondo Y, Izumi A, Yamamoto K, Ichikawa T, Tamura T, Noumi K, Yamashita Y, Morishita K.  Overexpression of absent in melanoma 2 in oral squamous cell carcinoma contributes to tumor progression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30587341
9696  Kazuya Hiroshima, Masashi Shiiba, Noritoshi Oka, Fumihiko Hayashi, Sho Ishida, Reo Fukushima, Kazuyuki Koike, Manabu Iyoda, Dai Nakashima, Hideki Tanzawa, Katsuhiro Uzawa  Tspan15 Plays a Crucial Role in Metastasis in Oral Squamous Cell Carcinoma  Exp Cell Res  2019  384(2):111622  PubMed ID: 31518558
11907  Cervino G, Fiorillo L, Herford AS, Romeo U, Bianchi A, Crimi S, D'Amico C, De Stefano R, Troiano G, Santoro R, Laino L, Laino G, Cicciù M.  Molecular Biomarkers Related to Oral Carcinoma: Clinical Trial Outcome Evaluation in a Literature Review.  Dis. Markers  2019    PubMed ID: 31019584
12586  Sato K, Shi L, Ito F, Ohara Y, Motooka Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Hibi H, Toyokuni S.  Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner  J Clin Biochem Nutr  2019  65(1):8-15  PubMed ID: 31379408
10682  Yamamoto JI, Kasamatsu A, Okubo Y, Nakashima D, Fushimi K, Minakawa Y, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Evaluation of tryptophan-aspartic acid repeat-containing protein 34 as a novel tumor-suppressor molecule in human oral cancer.  Biochem. Biophys. Res. Commun.  2018  495:2469-2474  PubMed ID: 29278705
10683  Suzuki S, Hoshino H, Yoshida K, Nakanishi J, Tsuchiya-Hirata S, Kobuke S, Haruyama N, Nishimura F, Shiba H.  Genome-wide identification of chromatin-enriched RNA reveals that unspliced dentin matrix protein-1 mRNA regulates cell proliferation in squamous cell carcinoma.  Biochem. Biophys. Res. Commun.  2018  495:2303-2309  PubMed ID: 29278708
10806  Okubo Y, Kasamatsu A, Yamatoji M, Fushimi K, Ishigami T, Shimizu T, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.  Exp. Cell Res.  2018  367:112-118  PubMed ID: 29614312
10820  Fukamachi M, Kasamatsu A, Endo-Sakamoto Y, Fushimi K, Kasama H, Iyoda M, Minakawa Y, Shiiba M, Tanzawa H, Uzawa K.  Multiple coagulation factor deficiency protein 2 as a crucial component in metastasis of human oral cancer.  Exp. Cell Res.  2018  368:119-125  PubMed ID: 29679592
11180  Toeda Y, Kasamatsu A, Koike K, Endo-Sakamoto Y, Fushimi K, Kasama H, Yamano Y, Shiiba M, Tanzawa H, Uzawa K.  FBLIM1 enhances oral cancer malignancy via modulation of the epidermal growth factor receptor pathway.  Mol. Carcinog.  2018  57(12):1690-1697  PubMed ID: 30129678
11192  Miyaguchi J, Shiga K, Ogawa K, Suzuki F, Katagiri K, Saito D, Ikeda A, Horii A, Watanabe M, Igimi S.  Treatment with Lactobacillus Retards the Tumor Growth of Head and Neck Squamous Cell Carcinoma Cells Inoculated in Mice.  Tohoku J. Exp. Med.  2018  245(4):269-275  PubMed ID: 30158369
11226  Fukushima R, Kasamatsu A, Nakashima D, Higo M, Fushimi K, Kasama H, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of Translocation Associated Membrane Protein 2 Leading to Cancer-Associated Matrix Metalloproteinase Activation as a Putative Metastatic Factor for Human Oral Cancer.  J Cancer  2018    PubMed ID: 30271493
4345  Hou B, Ishinaga H, Midorikawa K, Nakamura S, Hiraku Y, Oikawa S, Ma N, Takeuchi K, Murata M.  Let-7c inhibits migration and epithelial-mesenchymal transition in head and neck squamous cell carcinoma by targeting IGF1R and HMGA2.  Oncotarget  2018    PubMed ID: 29507664
10148  Takahara T, Kasamatsu A, Yamatoji M, Iyoda M, Kasama H, Saito T, Takeuchi S, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  SIPA1 promotes invasion and migration in human oral squamous cell carcinoma by ITGB1 and MMP7.  Exp. Cell Res.  2017  352:357-363  PubMed ID: 28237246
10155  Koide N, Kasamatsu A, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Evidence for Critical Role of Lymphocyte Cytosolic Protein 1 in Oral Cancer.  Sci Rep  2017  7:43379  PubMed ID: 28230172
10191  Takeuchi S, Kasamatsu A, Yamatoji M, Nakashima D, Endo-Sakamoto Y, Koide N, Takahara T, Shimizu T, Iyoda M, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  TEAD4-YAP interaction regulates tumoral growth by controlling cell-cycle arrest at the G1 phase.  Biochem. Biophys. Res. Commun.  2017  486:385-390  PubMed ID: 28315328
10418  Kita A, Kasamatsu A, Nakashima D, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis.  J Cancer  2017  8:2033-2041  PubMed ID: 28819404
11036    Increased Expression of CD44v9, A Cancer Stem Cell Marker, in Head And Neck Squamous Cell Carcinoma Cells after Irradiation  Int J Cancer Oncol  2017  4: 225- 230 
6027  lNorihisa Ichimura et al  Notch3 is frequently downregulated in oral cancer  Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   2017  29:504-510 
6029  Ishida S et al  Novel mechanism of aberrant ZIP4 expression with zinc supplementation in oral tumorigenesis.  Biochem Biophys Res Commun  2017  483(1):339-345  PubMed ID: 28017725
12679  Tamura T, Ichikawa T, Nakahata S, Kondo Y, Tagawa Y, Yamamoto K, Nagai K, Baba T, Yamaguchi R, Futakuchi M, Yamashita Y, Morishita K.  Loss of NDRG2 Expression Confers Oral Squamous Cell Carcinoma with Enhanced Metastatic Potential  Cancer Res  2017  77(9):2363-2374  PubMed ID: 28209617
9616  Kitajima D, Kasamatsu A, Nakashima D, Miyamoto I, Kimura Y, Saito T, Suzuki T, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Tie2 Regulates Tumor Metastasis of Oral Squamous Cell Carcinomas.  J Cancer  2016  7:600-7  PubMed ID: 27053959
9630  Kimura Y, Kasamatsu A, Nakashima D, Yamatoji M, Minakawa Y, Koike K, Fushimi K, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  ARNT2 Regulates Tumoral Growth in Oral Squamous Cell Carcinoma.  J Cancer  2016  7:702-10  PubMed ID: 27076852
6036  Suzuki T, Kasamatsu A, Miyamoto I, Saito T, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of TMOD1 is associated with enhanced regional lymph node metastasis in human oral cancer.  Int J Oncol.  2016  48(2):607-12  PubMed ID: 26718916
7442  Shirako Y, Taya Y, Sato K, Chiba T, Imai K, Shimazu Y, Aoba T, Soeno Y.  Heterogeneous tumor stromal microenvironments of oral squamous cell carcinoma cells in tongue and nodal metastatic lesions in a xenograft mouse model.  J. Oral Pathol. Med.  2015  44:656-68  PubMed ID: 25765182
6044  Kasama H et al   Adenosine A2b receptor promotes progression of human oral cancer.  BMC Cancer  2015  15:563  PubMed ID: 26228921
6051  Okamoto A et al  Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.  PLoS One  2015  10(11):e0142252  PubMed ID: 26554377
6052  Saito T et al  Semaphorin7A Promotion of Tumoral Growth and Metastasis in Human Oral Cancer by Regulation of G1 Cell Cycle and Matrix Metalloproteases: Possible Contribution to Tumoral Angiogenesis.  PLoS One  2015  10(9):e0137923  PubMed ID: 26378920
6058  Shida-Sakazume T et al  Lysophosphatidylcholine acyltransferase1 overexpression promotes oral squamous cell carcinoma progression via enhanced biosynthesis of platelet-activating factor.  PLoS One.  2015  10(3):e0120143  PubMed ID: 25803864
5028  Saito Y, Kasamatsu A, Yamamoto A, Shimizu T, Yokoe H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  ALY as a potential contributor to metastasis in human oral squamous cell carcinoma.  J Cancer Res Clin Oncol  2013  139(4):585-94  PubMed ID: 23242234
6067  Koike K, Kasamatsu A, Iyoda M, Saito Y, Kouzu Y, Koike H, Sakamoto Y, Ogawara K, Tanzawa H, Uzawa K.  High prevalence of epigenetic inactivation of the human four and a half LIM domains 1 gene in human oral cancer.  Int J Oncol  2013  42(1):141-50  PubMed ID: 23123766
6070  Yamamoto A, Kasamatsu A, Ishige S, Koike K, Saito K, Kouzu Y, Koike H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  Exocyst complex component Sec8: a presumed component in the progression of human oral squamous-cell carcinoma by secretion of matrix metalloproteinases.  J Cancer Res Clin Oncol  2013  139(4):533-42  PubMed ID: 23207790
6072  Uchida F, Uzawa K, Kasamatsu A, Takatori H, Sakamoto Y, Ogawara K, Shiiba M, Bukawa H, Tanzawa H.  Overexpression of CDCA2 in human squamous cell carcinoma: correlation with prevention of G1 phase arrest and apoptosis.  PLoS One  2013  8(2):e56381  PubMed ID: 23418564
6074  Shimizu F1, Shiiba M2, Ogawara K3, Kimura R1, Minakawa Y1, Baba T1, Yokota S, Nakashima D1, Higo M3, Kasamatsu A3, Sakamoto Y3, Tanzawa H4, Uzawa K4.  Overexpression of LIM and SH3 Protein 1 leading to accelerated G2/M phase transition contributes to enhanced tumourigenesis in oral cancer.  PLoS One  2013  8(12):e83187  PubMed ID: 24386158
6076  Minakawa Y, Kasamatsu A, Koike H, Higo M, Nakashima D, Kouzu Y, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  Kinesin family member 4A: a potential predictor for progression of human oral cancer.  PLoS One. 2  2013  8(12):e85951  PubMed ID: 24386490
6077  Mikami T, Hada T, Chosa N, Ishisaki A, Mizuki H, Takeda Y. Author information  Expression of Wilms' tumor 1 (WT1) in oral squamous cell carcinoma.  J Oral Pathol Med  2013  42(2):133-9  PubMed ID: 22672247
6081  Kimura R, Kasamatsu A, Koyama T, Fukumoto C, Kouzu Y, Higo M, Endo-Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation.  BMC Cancer  2013  13:555  PubMed ID: 24261884
6083  Kumai T, Matsuda Y, Oikawa K, Aoki N, Kimura S, Harabuchi Y, Celis E, Kobayashi H.  EGFR inhibitors augment antitumour helper T-cell responses of HER family-specific immunotherapy.  Br J Cancer  2013  109(8):2155-66  PubMed ID: 24045666
6110  YOSHIAKI TABUCHI, YUKIHIRO FURUSAWA, SHIGEHITO WADA, KENZO OHTSUKA, TAKASHI KONDO  Silencing Heat Shock Transcription Factor 1 Using Small Interfering RNA Enhances Mild Hyperthermia and Hyperthermia Sensitivity in Human Oral Squamous Cell Carcinoma Cells  Thermal Medicine  2011  27:99-108 
8284  Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M.  Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22.  J. Periodont. Res.  2010  45:488-95  PubMed ID: 20412422
6120  Furuta H, Kondo Y, Nakahata S, Hamasaki M, Sakoda S, Morishita K.  NDRG2 is a candidate tumor-suppressor for oral squamous-cell carcinoma.  Biochem Biophys Res Commun  2010  391(4):1785-91  PubMed ID: 20045673
6122  Nomi N, Kodama S, Suzuki M.  Toll-like receptor 3 signaling induces apoptosis in human head and neck cancer via survivin associated pathway.  Oncol Rep  2010  24(1):225-31  PubMed ID: 20514466
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309

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