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Cell No. : Cell Name
RCB2102 : HO-1-u-1  update : 2022/07/07
CommentHuman cell line derived from squamous cell carcinoma at mouth floor. TKG0455 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Miyauchi, T.
Year of deposit 2005
Original cell TKG0455
Animal human < Mammals
Genus Homo
Species sapiens
Tissue mouth floor
Disease name Squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0455)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2784
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 53


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Reference
5088  Sugiyama A, Ishiguro M, Yasue T, Watanabe S, Sugishita N, Yokoyama H, Uno T, Watanabe H, Matsuno Y, Sasaoka I.  An autopsy case of triple cancer associated with superior vena cava syndrome  Gan No Rinsho  1985  31(10):1347-52  PubMed ID: 2997498  

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User's Publication
20559  Komatsu M, Saito K, Miyamoto I, Koike K, Iyoda M, Nakashima D, Kasamatsu A, Shiiba M, Tanzawa H, Uzawa K.  Aberrant GIMAP2 expression affects oral squamous cell carcinoma progression by promoting cell cycle and inhibiting apoptosis.  Oncol Lett  2022  23(2):49  PubMed ID: 34992682   DOI: 10.3892/ol.2021.13167
20020  Shishido K, Kuroishi T, Sugawara S.  P2 purinergic receptor signaling and interleukin-1 synergistically induce interleukin-6 production in a human oral squamous carcinoma cell line.  J Oral Biosci  2021    PubMed ID: 33497843   DOI: 10.1016/j.job.2021.01.004
20085  Kase Y, Uzawa K, Wagai S, Yoshimura S, Yamamoto JI, Toeda Y, Okubo M, Eizuka K, Ando T, Nobuchi T, Kawasaki K, Saito T, Iyoda M, Nakashima D, Kasamatsu A, Tanzawa H.  Engineered exosomes delivering specific tumor-suppressive RNAi attenuate oral cancer progression.  Sci Rep  2021  11(1):5897  PubMed ID: 33723306   DOI: 10.1038/s41598-021-85242-1
16206  Koma A, Asai S, Minemura C, Oshima S, Kinoshita T, Kikkawa N, Koshizuka K, Moriya S, Kasamatsu A, Hanazawa T, Uzawa K, Seki N.  Impact of Oncogenic Targets by Tumor-Suppressive miR-139-5p and miR-139-3p Regulation in Head and Neck Squamous Cell Carcinoma  Int J Mol Sci  2021  22(18):9947  PubMed ID: 34576110   DOI: 10.3390/ijms22189947
16975  Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K.  Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling  Anticancer Res  2021  41(4):1821-1830  PubMed ID: 33813387   DOI: 10.21873/anticanres.14948
13903  Aika Tanzawa et al.  Comprehensive gene expression analysis of semaphorins in oral squamous cell carcinoma  Oral Science International  2020  17(2):67-72    DOI: 10.1002/osi2.1044
11354  Nakamura Y, Nakahata S, Kondo Y, Izumi A, Yamamoto K, Ichikawa T, Tamura T, Noumi K, Yamashita Y, Morishita K.  Overexpression of absent in melanoma 2 in oral squamous cell carcinoma contributes to tumor progression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30587341   DOI: 10.1016/j.bbrc.2018.12.066
9696  Kazuya Hiroshima, Masashi Shiiba, Noritoshi Oka, Fumihiko Hayashi, Sho Ishida, Reo Fukushima, Kazuyuki Koike, Manabu Iyoda, Dai Nakashima, Hideki Tanzawa, Katsuhiro Uzawa  Tspan15 Plays a Crucial Role in Metastasis in Oral Squamous Cell Carcinoma  Exp Cell Res  2019  384(2):111622  PubMed ID: 31518558   DOI: 10.1016/j.yexcr.2019.111622
11907  Cervino G, Fiorillo L, Herford AS, Romeo U, Bianchi A, Crimi S, D'Amico C, De Stefano R, Troiano G, Santoro R, Laino L, Laino G, Cicciù M.  Molecular Biomarkers Related to Oral Carcinoma: Clinical Trial Outcome Evaluation in a Literature Review.  Dis. Markers  2019    PubMed ID: 31019584   DOI: 10.1155/2019/8040361
12586  Sato K, Shi L, Ito F, Ohara Y, Motooka Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Hibi H, Toyokuni S.  Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner  J Clin Biochem Nutr  2019  65(1):8-15  PubMed ID: 31379408   DOI: 10.3164/jcbn.18-91
10682  Yamamoto JI, Kasamatsu A, Okubo Y, Nakashima D, Fushimi K, Minakawa Y, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Evaluation of tryptophan-aspartic acid repeat-containing protein 34 as a novel tumor-suppressor molecule in human oral cancer.  Biochem. Biophys. Res. Commun.  2018  495:2469-2474  PubMed ID: 29278705   DOI: 10.1016/j.bbrc.2017.12.138
10683  Suzuki S, Hoshino H, Yoshida K, Nakanishi J, Tsuchiya-Hirata S, Kobuke S, Haruyama N, Nishimura F, Shiba H.  Genome-wide identification of chromatin-enriched RNA reveals that unspliced dentin matrix protein-1 mRNA regulates cell proliferation in squamous cell carcinoma.  Biochem. Biophys. Res. Commun.  2018  495:2303-2309  PubMed ID: 29278708   DOI: 10.1016/j.bbrc.2017.12.136
10806  Okubo Y, Kasamatsu A, Yamatoji M, Fushimi K, Ishigami T, Shimizu T, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.  Exp. Cell Res.  2018  367:112-118  PubMed ID: 29614312   DOI: 10.1016/j.yexcr.2018.03.041
10820  Fukamachi M, Kasamatsu A, Endo-Sakamoto Y, Fushimi K, Kasama H, Iyoda M, Minakawa Y, Shiiba M, Tanzawa H, Uzawa K.  Multiple coagulation factor deficiency protein 2 as a crucial component in metastasis of human oral cancer.  Exp. Cell Res.  2018  368:119-125  PubMed ID: 29679592   DOI: 10.1016/j.yexcr.2018.04.021
11180  Toeda Y, Kasamatsu A, Koike K, Endo-Sakamoto Y, Fushimi K, Kasama H, Yamano Y, Shiiba M, Tanzawa H, Uzawa K.  FBLIM1 enhances oral cancer malignancy via modulation of the epidermal growth factor receptor pathway.  Mol. Carcinog.  2018  57(12):1690-1697  PubMed ID: 30129678   DOI: 10.1002/mc.22889
11192  Miyaguchi J, Shiga K, Ogawa K, Suzuki F, Katagiri K, Saito D, Ikeda A, Horii A, Watanabe M, Igimi S.  Treatment with Lactobacillus Retards the Tumor Growth of Head and Neck Squamous Cell Carcinoma Cells Inoculated in Mice.  Tohoku J. Exp. Med.  2018  245(4):269-275  PubMed ID: 30158369   DOI: 10.1620/tjem.245.269
11226  Fukushima R, Kasamatsu A, Nakashima D, Higo M, Fushimi K, Kasama H, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of Translocation Associated Membrane Protein 2 Leading to Cancer-Associated Matrix Metalloproteinase Activation as a Putative Metastatic Factor for Human Oral Cancer.  J Cancer  2018    PubMed ID: 30271493   DOI: 10.7150/jca.25666
4345  Hou B, Ishinaga H, Midorikawa K, Nakamura S, Hiraku Y, Oikawa S, Ma N, Takeuchi K, Murata M.  Let-7c inhibits migration and epithelial-mesenchymal transition in head and neck squamous cell carcinoma by targeting IGF1R and HMGA2.  Oncotarget  2018    PubMed ID: 29507664   DOI: 10.18632/oncotarget.23826
16201  Sawai Y, Kasamatsu A, Nakashima D, Fushimi K, Kasama H, Iyoda M, Kouzu Y, Shiiba M, Tanzawa H, Uzawa K.  Critical role of deoxynucleotidyl transferase terminal interacting protein 1 in oral cancer  Lab Invest  2018  98(8):980-988  PubMed ID: 29855544   DOI: 10.1038/s41374-018-0070-3
10148  Takahara T, Kasamatsu A, Yamatoji M, Iyoda M, Kasama H, Saito T, Takeuchi S, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  SIPA1 promotes invasion and migration in human oral squamous cell carcinoma by ITGB1 and MMP7.  Exp. Cell Res.  2017  352:357-363  PubMed ID: 28237246   DOI: 10.1016/j.yexcr.2017.02.026
10155  Koide N, Kasamatsu A, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Evidence for Critical Role of Lymphocyte Cytosolic Protein 1 in Oral Cancer.  Sci Rep  2017  7:43379  PubMed ID: 28230172   DOI: 10.1038/srep43379
10191  Takeuchi S, Kasamatsu A, Yamatoji M, Nakashima D, Endo-Sakamoto Y, Koide N, Takahara T, Shimizu T, Iyoda M, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  TEAD4-YAP interaction regulates tumoral growth by controlling cell-cycle arrest at the G1 phase.  Biochem. Biophys. Res. Commun.  2017  486:385-390  PubMed ID: 28315328   DOI: 10.1016/j.bbrc.2017.03.050
10418  Kita A, Kasamatsu A, Nakashima D, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis.  J Cancer  2017  8:2033-2041  PubMed ID: 28819404   DOI: 10.7150/jca.18714
11036    Increased Expression of CD44v9, A Cancer Stem Cell Marker, in Head And Neck Squamous Cell Carcinoma Cells after Irradiation  Int J Cancer Oncol  2017  4: 225- 230    DOI: 10.15436/2377-0902.17.1628
6027  lNorihisa Ichimura et al  Notch3 is frequently downregulated in oral cancer  Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   2017  29:504-510    DOI: 10.1016/j.ajoms.2017.06.006
6029  Ishida S et al  Novel mechanism of aberrant ZIP4 expression with zinc supplementation in oral tumorigenesis.  Biochem Biophys Res Commun  2017  483(1):339-345  PubMed ID: 28017725   DOI: 10.1016/j.bbrc.2016.12.142
12679  Tamura T, Ichikawa T, Nakahata S, Kondo Y, Tagawa Y, Yamamoto K, Nagai K, Baba T, Yamaguchi R, Futakuchi M, Yamashita Y, Morishita K.  Loss of NDRG2 Expression Confers Oral Squamous Cell Carcinoma with Enhanced Metastatic Potential  Cancer Res  2017  77(9):2363-2374  PubMed ID: 28209617   DOI: 10.1158/0008-5472.CAN-16-2114
14306  Noriko Komatsu, Kenichi Mitsui, Osamu Kusano-Arai, Hiroko Iwanari, Kazuto Hoshi, Tsuyoshi Takato, Takahiro Abe and Takao Hamakubo  Enhancement of anti-robo1 immunotoxin cytotoxicity to head and neck squamous cell carcinoma via photochemical internalization  Archives in Cancer Research  2017  5   
9616  Kitajima D, Kasamatsu A, Nakashima D, Miyamoto I, Kimura Y, Saito T, Suzuki T, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Tie2 Regulates Tumor Metastasis of Oral Squamous Cell Carcinomas.  J Cancer  2016  7:600-7  PubMed ID: 27053959   DOI: 10.7150/jca.13820
9630  Kimura Y, Kasamatsu A, Nakashima D, Yamatoji M, Minakawa Y, Koike K, Fushimi K, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  ARNT2 Regulates Tumoral Growth in Oral Squamous Cell Carcinoma.  J Cancer  2016  7:702-10  PubMed ID: 27076852   DOI: 10.7150/jca.14208
6036  Suzuki T, Kasamatsu A, Miyamoto I, Saito T, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of TMOD1 is associated with enhanced regional lymph node metastasis in human oral cancer.  Int J Oncol.  2016  48(2):607-12  PubMed ID: 26718916   DOI: 10.3892/ijo.2015.3305
7442  Shirako Y, Taya Y, Sato K, Chiba T, Imai K, Shimazu Y, Aoba T, Soeno Y.  Heterogeneous tumor stromal microenvironments of oral squamous cell carcinoma cells in tongue and nodal metastatic lesions in a xenograft mouse model.  J. Oral Pathol. Med.  2015  44:656-68  PubMed ID: 25765182   DOI: 10.1111/jop.12318
6044  Kasama H et al   Adenosine A2b receptor promotes progression of human oral cancer.  BMC Cancer  2015  15:563  PubMed ID: 26228921   DOI: 10.1186/s12885-015-1577-2
6051  Okamoto A et al  Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.  PLoS One  2015  10(11):e0142252  PubMed ID: 26554377   DOI: 10.1371/journal.pone.0142252
6052  Saito T et al  Semaphorin7A Promotion of Tumoral Growth and Metastasis in Human Oral Cancer by Regulation of G1 Cell Cycle and Matrix Metalloproteases: Possible Contribution to Tumoral Angiogenesis.  PLoS One  2015  10(9):e0137923  PubMed ID: 26378920   DOI: 10.1371/journal.pone.0137923
6058  Shida-Sakazume T et al  Lysophosphatidylcholine acyltransferase1 overexpression promotes oral squamous cell carcinoma progression via enhanced biosynthesis of platelet-activating factor.  PLoS One.  2015  10(3):e0120143  PubMed ID: 25803864   DOI: 10.1371/journal.pone.0120143
14987  Kaneko T, Kanno C, Ichikawa-Tomikawa N, Kashiwagi K, Yaginuma N, Ohkoshi C, Tanaka M, Sugino T, Imura T, Hasegawa H, Chiba H.  Liver X receptor reduces proliferation of human oral cancer cells by promoting cholesterol efflux via up-regulation of ABCA1 expression  Oncotarget  2015  6(32):33345-57  PubMed ID: 26452260   DOI: 10.18632/oncotarget.5428
15042  Koyama T, Ogawara K, Kasamatsu A, Okamoto A, Kasama H, Minakawa Y, Shimada K, Yokoe H, Shiiba M, Tanzawa H, Uzawa K.  ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer  Cancer Med  2015  4(5):759-69  PubMed ID: 25644496   DOI: 10.1002/cam4.418
16010  Fukumoto C, Nakashima D, Kasamatsu A, Unozawa M, Shida-Sakazume T, Higo M, Ogawara K, Yokoe H, Shiiba M, Tanzawa H, Uzawa K.  WWP2 is overexpressed in human oral cancer, determining tumor size and poor prognosis in patients: downregulation of WWP2 inhibits the AKT signaling and tumor growth in mice  Oncoscience  2014  1(12):807-20  PubMed ID: 25621296   DOI: 10.18632/oncoscience.101
5028  Saito Y, Kasamatsu A, Yamamoto A, Shimizu T, Yokoe H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  ALY as a potential contributor to metastasis in human oral squamous cell carcinoma.  J Cancer Res Clin Oncol  2013  139(4):585-94  PubMed ID: 23242234   DOI: 10.1007/s00432-012-1361-5
6067  Koike K, Kasamatsu A, Iyoda M, Saito Y, Kouzu Y, Koike H, Sakamoto Y, Ogawara K, Tanzawa H, Uzawa K.  High prevalence of epigenetic inactivation of the human four and a half LIM domains 1 gene in human oral cancer.  Int J Oncol  2013  42(1):141-50  PubMed ID: 23123766   DOI: 10.3892/ijo.2012.1677
6070  Yamamoto A, Kasamatsu A, Ishige S, Koike K, Saito K, Kouzu Y, Koike H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  Exocyst complex component Sec8: a presumed component in the progression of human oral squamous-cell carcinoma by secretion of matrix metalloproteinases.  J Cancer Res Clin Oncol  2013  139(4):533-42  PubMed ID: 23207790   DOI: 10.1007/s00432-012-1356-2
6072  Uchida F, Uzawa K, Kasamatsu A, Takatori H, Sakamoto Y, Ogawara K, Shiiba M, Bukawa H, Tanzawa H.  Overexpression of CDCA2 in human squamous cell carcinoma: correlation with prevention of G1 phase arrest and apoptosis.  PLoS One  2013  8(2):e56381  PubMed ID: 23418564   DOI: 10.1371/journal.pone.0056381
6074  Shimizu F1, Shiiba M2, Ogawara K3, Kimura R1, Minakawa Y1, Baba T1, Yokota S, Nakashima D1, Higo M3, Kasamatsu A3, Sakamoto Y3, Tanzawa H4, Uzawa K4.  Overexpression of LIM and SH3 Protein 1 leading to accelerated G2/M phase transition contributes to enhanced tumourigenesis in oral cancer.  PLoS One  2013  8(12):e83187  PubMed ID: 24386158   DOI: 10.1371/journal.pone.0083187
6076  Minakawa Y, Kasamatsu A, Koike H, Higo M, Nakashima D, Kouzu Y, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  Kinesin family member 4A: a potential predictor for progression of human oral cancer.  PLoS One. 2  2013  8(12):e85951  PubMed ID: 24386490   DOI: 10.1371/journal.pone.0085951
6077  Mikami T, Hada T, Chosa N, Ishisaki A, Mizuki H, Takeda Y. Author information  Expression of Wilms' tumor 1 (WT1) in oral squamous cell carcinoma.  J Oral Pathol Med  2013  42(2):133-9  PubMed ID: 22672247   DOI: 10.1111/j.1600-0714.2012.01182.x
6081  Kimura R, Kasamatsu A, Koyama T, Fukumoto C, Kouzu Y, Higo M, Endo-Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation.  BMC Cancer  2013  13:555  PubMed ID: 24261884   DOI: 10.1186/1471-2407-13-555
6083  Kumai T, Matsuda Y, Oikawa K, Aoki N, Kimura S, Harabuchi Y, Celis E, Kobayashi H.  EGFR inhibitors augment antitumour helper T-cell responses of HER family-specific immunotherapy.  Br J Cancer  2013  109(8):2155-66  PubMed ID: 24045666   DOI: 10.1038/bjc.2013.577
6110  YOSHIAKI TABUCHI, YUKIHIRO FURUSAWA, SHIGEHITO WADA, KENZO OHTSUKA, TAKASHI KONDO  Silencing Heat Shock Transcription Factor 1 Using Small Interfering RNA Enhances Mild Hyperthermia and Hyperthermia Sensitivity in Human Oral Squamous Cell Carcinoma Cells  Thermal Medicine  2011  27:99-108    DOI: 10.3191/thermalmed.27.99
8284  Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M.  Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22.  J. Periodont. Res.  2010  45:488-95  PubMed ID: 20412422   DOI: 10.1111/j.1600-0765.2009.01263.x
6120  Furuta H, Kondo Y, Nakahata S, Hamasaki M, Sakoda S, Morishita K.  NDRG2 is a candidate tumor-suppressor for oral squamous-cell carcinoma.  Biochem Biophys Res Commun  2010  391(4):1785-91  PubMed ID: 20045673   DOI: 10.1016/j.bbrc.2009.12.156
6122  Nomi N, Kodama S, Suzuki M.  Toll-like receptor 3 signaling induces apoptosis in human head and neck cancer via survivin associated pathway.  Oncol Rep  2010  24(1):225-31  PubMed ID: 20514466   DOI: 10.3892/or_00000850
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x



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