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Cell No. : Cell Name
RCB2093 : II-18  update : 2018/08/02
CommentHuman cell line derived from lung cancer. Adenocarcinoma. TKG0177 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
approver's address
Japan Anti-Tuberculosis Association MIYAGI

2-3-1 Nakayamayoshinari, Aoba-ku, Sendai, Miyagi 989-3203 Japan
Dr.Kobayashi, Syunsuke
Fax. +81-22-719-5166
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Kobayashi, Syunsuke
Year of deposit 2005
Original cell TKG0177
Animal human < Mammals
Genus homo
Species sapiens
Tissue lung
Case history adenocarcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0177)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 5

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3447      Kokenshi  1990  42:73-79 

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User's Publication
11040  Koh Furugaki, Marie Mochizuki, Mirei Kohno, Sei Shu, Naoki Harada, Yasushi Yoshimura   Expression of C-terminal ALK, RET, or ROS1 in lung cancer cells with or without fusion  BMC Cancer  2019  19(1):301  PubMed ID: 30943926
10768  Kashima Y, Suzuki A, Liu Y, Hosokawa M, Matsunaga H, Shirai M, Arikawa K, Sugano S, Kohno T, Takeyama H, Tsuchihara K, Suzuki Y.  Combinatory use of distinct single-cell RNA-seq analytical platforms reveals the heterogeneous transcriptome response.  Sci Rep  2018  8:3482  PubMed ID: 29472726
10411  Kunimasa K, Nagano T, Shimono Y, Dokuni R, Kiriu T, Tokunaga S, Tamura D, Yamamoto M, Tachihara M, Kobayashi K, Satouchi M, Nishimura Y.  Glucose metabolism-targeted therapy and withaferin A are effective for epidermal growth factor receptor tyrosine kinase inhibitor-induced drug-tolerant persisters.  Cancer Sci.  2017  108:1368-1377  PubMed ID: 28445002
8277  Shirogane Y, Takeda M, Tahara M, Ikegame S, Nakamura T, Yanagi Y.  Epithelial-mesenchymal transition abolishes the susceptibility of polarized epithelial cell lines to measles virus.  J. Biol. Chem.  2010  285:20882-90  PubMed ID: 20435897
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309

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