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Cell No. : Cell Name
RCB2084 : TALL-1  update : 2022/09/30
CommentHuman T-cell line derived from acute lynphblastic leukemia. TKG0251 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Hiraki
Year of deposit 2005
Original cell TKG0251
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue bone marrow
Disease name T-cell leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG00251)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1736
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 2


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Reference
5073  Hiraki S, Miyoshi I, Kubonishi I, Matsuda Y, Nakayama T, Kishimoto H, Machida K, Masuji H, Kimura I.  Establishment of a T-cell line from human lymphosarcoma.  Gan  1978  69(1):115-8  PubMed ID: 305872  
621  Miyoshi I, Hiraki S, Tsubota T, Kubonishi I, Matsuda Y, Nakayama T, Kishimoto H, Kimura I, Masuji H.  Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias.  Nature  1977  267(5614):843-4  PubMed ID: 197412   DOI: 10.1038/267843a0

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User's Publication
13384  Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.  Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.  Oncol Lett  2021    PubMed ID: 33376545   DOI: 10.3892/ol.2020.12374
14532  Doi K, Imai T, Kressler C, Yagita H, Agata Y, Vooijs M, Hamazaki Y, Inoue J, Minato N.  Crucial role of the Rap G protein signal in Notch activation and leukemogenicity of T-cell acute lymphoblastic leukemia  Sci Rep  2015  5:7978  PubMed ID: 25613394   DOI: 10.1038/srep07978



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