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Cell No. : Cell Name
RCB2081 : KUSA-A1  update : 2022/10/27
CommentMesenchymal stem cell line derived from C3H/He mouse strain.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal mouse < Mammals
Strain name C3H/He
Gender Female
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4848
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 23
User's Publication 8


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Reference
2542  Akon Higuchi, Qing-Dong Ling, S. Suresh Kumar, Yung Chang, Ta-Chun Kao, Murugan A. Munusamy, Abdullah A. Alarfaj, Shih-Tien Hsu, Akihiro Umezawa  External stimulus-responsive biomaterials designed for the culture and differentiation of ES, iPS, and adult stem cells  Progress in Polymer Science  2014  39:1585-1613    DOI: 10.1016/j.progpolymsci.2014.05.001
8027  Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.  Lectin microarray analysis of pluripotent and multipotent stem cells.  Genes Cells  2011  16:1-11  PubMed ID: 21155951   DOI: 10.1111/j.1365-2443.2010.01459.x
2523  Wang S, Kawashima N, Sakamoto K, Katsube K, Umezawa A, Suda H.  Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor Rbpj  Biochem Biophys Res Commun  2010  400(1):39-45  PubMed ID: 20691157   DOI: 10.1016/j.bbrc.2010.07.133
2540  Toyoda M, Hamatani T, Okada H, Matsumoto K, Saito H, Umezawa A.  Defining cell identity by comprehensive gene expression profiling  Curr Med Chem  2010  17(28):3245-52  PubMed ID: 20666720   DOI: 10.2174/092986710792231932
2536  Katsube K, Ichikawa S, Katsuki Y, Kihara T, Terai M, Lau LF, Tamamura Y, Takeda S, Umezawa A, Sakamoto K, Yamaguchi A.  CCN3 and bone marrow cells  J Cell Commun Signal  2009  3(2):135-45  PubMed ID: 19626464   DOI: 10.1007/s12079-009-0059-1
2513  Katsuki Y, Sakamoto K, Minamizato T, Makino H, Umezawa A, Ikeda MA, Perbal B, Amagasa T, Yamaguchi A, Katsube K.  Inhibitory effect of CT domain of CCN3/NOV on proliferation and differentiation of osteogenic mesenchymal stem cells, Kusa-A1  Biochem Biophys Res Commun  2008  368(3):808-14  PubMed ID: 18275847   DOI: 10.1016/j.bbrc.2008.02.010
1682  Kondo S, Shukunami C, Morioka Y, Matsumoto N, Takahashi R, Oh J, Atsumi T, Umezawa A, Kudo A, Kitayama H, Hiraki Y, Noda M.  Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells.  J Cell Sci  2007  120(Pt 5):849-57  PubMed ID: 17298979   DOI: 10.1242/jcs.03388
2541  Akihiro Umezawa, Masashi Toyoda  Two MSCs: Marrow stromal cells and mesenchymal stem cells  Inflammation and Regeneration  2007  27:28-36    DOI: 10.2492/inflammregen.27.28
2535  Nagayoshi K, Ohkawa H, Yorozu K, Higuchi M, Higashi S, Kubota N, Fukui H, Imai N, Gojo S, Hata J, Kobayashi Y, Umezawa A.  Increased mobilization of c-kit+ Sca-1+ Lin- (KSL) cells and colony-forming units in spleen (CFU-S) following de novo formation of a stem cell niche depends on dynamic, but not stable, membranous ossification  J Cell Physiol  2006  208(1):188-94  PubMed ID: 16575918   DOI: 10.1002/jcp.20652
2514  Kawashima N, Shindo K, Sakamoto K, Kondo H, Umezawa A, Kasugai S, Perbal B, Suda H, Takagi M, Katsube K.  Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa  J Bone Miner Metab  2005  23(2):123-33  PubMed ID: 15750690   DOI: 10.1007/s00774-004-0550-y
2517  Matsumoto S, Shibuya I, Kusakari S, Segawa K, Uyama T, Shimada A, Umezawa A.  Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts  Biochim Biophys Acta  2005  1725(1):57-63  PubMed ID: 15996824   DOI: 10.1016/j.bbagen.2005.05.027
2532  Higuchi A, Shindo Y, Gomei Y, Mori T, Uyama T, Umezawa A.  Cell separation between mesenchymal progenitor cells through porous polymeric membranes  J Biomed Mater Res B Appl Biomater  2005  74(1):511-9  PubMed ID: 15906389   DOI: 10.1002/jbm.b.30220
2525  Higuchi A, Hamamura A, Shindo Y, Kitamura H, Yoon BO, Mori T, Uyama T, Umezawa A.  Photon-modulated changes of cell attachments on poly(spiropyran-co-methyl methacrylate) membranes  Biomacromolecules  2004  5(5):1770-4  PubMed ID: 15360286   DOI: 10.1021/bm049737x
2537  Sakurai K, Iizuka S, Shen JS, Meng XL, Mori T, Umezawa A, Ohashi T, Eto Y.  Brain transplantation of genetically modified bone marrow stromal cells corrects CNS pathology and cognitive function in MPS VII mice  Gene Ther  2004  11(19):1475-81.  PubMed ID: 15295619   DOI: 10.1038/sj.gt.3302338
2538  K Sakurai, S Iizuka, J-S Shen, X-L Meng, T Mori, A Umezawa, T Ohashi, Y Eto  Osteogenesis with Cells and Scaffold.  Gene Therapy  2004  11:1475-1481    DOI: 10.2485/jhtb.14.255
2539  Tsuchiya K, Mori T, Chen G, Ushida T, Tateishi T, Matsuno T, Sakamoto M, Umezawa A.  Custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet  Cell Tissue Res  2004  316(2):141-53  PubMed ID: 14999559   DOI: 10.1007/s00441-004-0862-1
2512  Shindo K, Kawashima N, Sakamoto K, Yamaguchi A, Umezawa A, Takagi M, Katsube K, Suda H.  Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling  Exp Cell Res  2003  290(2):370-80  PubMed ID: 14567994   DOI: 10.1016/s0014-4827(03)00349-5
2522  Yoneda S, Itoh D, Kuroda S, Kondo H, Umezawa A, Ohya K, Ohyama T, Kasugai S.  The effects of enamel matrix derivative (EMD) on osteoblastic cells in culture and bone regeneration in a rat skull defect  J Periodontal Res  2003  38(3):333-42  PubMed ID: 12753373   DOI: 10.1034/j.1600-0765.2003.00667.x
2534  Ochi K, Chen G, Ushida T, Gojo S, Segawa K, Tai H, Ueno K, Ohkawa H, Mori T, Yamaguchi A, Toyama Y, Hata J, Umezawa A.  Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge  J Cell Physiol  2003  194(1):45-53  PubMed ID: 12447988   DOI: 10.1002/jcp.10185
2543  Gojo S, Umezawa A.  Plasticity of mesenchymal stem cells--regenerative medicine for diseased hearts  Hum Cell  2003  16(1):23-30  PubMed ID: 12971622   DOI: 10.1111/j.1749-0774.2003.tb00125.x
2526  Itoh D, Yoneda S, Kuroda S, Kondo H, Umezawa A, Ohya K, Ohyama T, Kasugai S.  Enhancement of osteogenesis on hydroxyapatite surface coated with synthetic peptide (EEEEEEEPRGDT) in vitro  J Biomed Mater Res  2002  62(2):292-8  PubMed ID: 12209950   DOI: 10.1002/jbm.10338
2754  Kohyama J, Abe H, Shimazaki T, Koizumi A, Nakashima K, Gojo S, Taga T, Okano H, Hata J, Umezawa A.  Brain from bone: efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent.  Differentiation  2001  68(4-5):235-44  PubMed ID: 11776476   DOI: 10.1046/j.1432-0436.2001.680411.x
2753  Umezawa A, Maruyama T, Segawa K, Shadduck RK, Waheed A, Hata J  Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.  J Cell Physiol  1992  151:197-205  PubMed ID: 1373147  

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User's Publication
20044  Goto R, Nishida E, Kobayashi S, Aino M, Ohno T, Iwamura Y, Kikuchi T, Hayashi JI, Yamamoto G, Asakura M, Mitani A.  Gelatin Methacryloyl-Riboflavin (GelMA-RF) Hydrogels for Bone Regeneration.  Int J Mol Sci  2021  22(4)  PubMed ID: 33561941   DOI: 10.3390/ijms22041635
20073  Phyo YS, Hashimoto K, Kawashima N, Kuramoto M, Okiji T.  Evaluation of the cytocompatibility of methacrylate resin-based root canal sealers with osteoblast-like cells.  Dent Mater J  2021    PubMed ID: 33716278   DOI: 10.4012/dmj.2020-335
13634  Kawashima N, Hashimoto K, Kuramoto M, Bakhit A, Wakabayashi Y, Okiji T.  A Novel Bioactive Endodontic Sealer Containing Surface-Reaction-Type Prereacted Glass-Ionomer Filler Induces Osteoblast Differentiation  Materials (Basel)  2020  13(20):4477  PubMed ID: 33050334   DOI: 10.3390/ma13204477
14981  Hashimoto A, Yamaguchi Y, Chiu LD, Morimoto C, Fujita K, Takedachi M, Kawata S, Murakami S, Tamiya E.  Time-lapse Raman imaging of osteoblast differentiation  Sci Rep  2015  5:12529  PubMed ID: 26211729   DOI: 10.1038/srep12529
15433  Kishi Y, Fujihara H, Kawaguchi K, Yamada H, Nakayama R, Yamamoto N, Fujihara Y, Hamada Y, Satomura K, Masutani M.  PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway  Int J Mol Sci   2015  16(10):24820-38  PubMed ID: 26492236   DOI: 10.3390/ijms161024820
17195  Shigeki Hontsu, Yoshiya Hashimoto, Yoshihiro Yoshikawa, Masanobu Kusunoki, Hiroaki Nishikawa, Akihiro Ametani  Fabrication of Hydroxyl Apatite Coating Titanium Web Scaffold Using Pulsed Laser Deposition Method  Journal of Hard Tissue Biology  2012  21:181-188    DOI: 10.2485/jhtb.21.181
8577  Silvia Smaldone, Luca Carta, Francesco Ramirez  Establishment of Fibrillin-Deficient Osteoprogenitor Cell Lines Identifies Molecular Abnormalities Associated With Extracellular Matrix Perturbation of Osteogenic Differentiation  Cell Tissue Res  2011  344(3):511-7  PubMed ID: 21538048   DOI: 10.1007/s00441-011-1167-9
8674  Hashimoto Y, Adachi S, Matsuno T, Omata K, Yoshitaka Y, Ozeki Y, Umezu Y, Satoh T, Nakamura M.  Effect of an injectable 3D scaffold for osteoblast differentiation depends on bead size.  Biomed Mater Eng  2009  19:391-400  PubMed ID: 20231791   DOI: 10.3233/BME-2009-0604



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