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Cell No. : Cell Name
RCB2080 : UCB408E6E7TERT-33  update : 2022/05/10
CommentHuman mesenchymal cell line derived from umbilical cord blood. Immortalized by HPV E6, E7, and hTERT.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal human < Mammals
Genus Homo
Species sapiens
Tissue umbilical cord blood
Classification transformed
Recombinant recombinant
Exogene HPV16 E6, HPV6 E7, hTERT
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3231
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives PLUSOID-M
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2 x 10^(5) cells/100 mm dish
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 3


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Reference
3476  Terai M1, Uyama T, Sugiki T, Li XK, Umezawa A, Kiyono T.  Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway.  Mol Biol Cell  2005  16(3):1491-9  PubMed ID: 15647378   DOI: 10.1091/mbc.e04-07-0652

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User's Publication
9398  Toru Fukuda 1, Hiroki Ochi 1, Satoko Sunamura 1, Akina Haide  MicroRNA-145 Regulates Osteoblastic Differentiation by Targeting the Transcription Factor Cbfb  FEBS Lett  2015  589(21):3302-8  PubMed ID: 26450370   DOI: 10.1016/j.febslet.2015.09.024
15074  Matsuzawa M, Arai C, Nomura Y, Murata T, Yamakoshi Y, Oida S, Hanada N, Nakamura Y.  Periostin of human periodontal ligament fibroblasts promotes migration of human mesenchymal stem cell through the αvβ3 integrin/FAK/PI3K/Akt pathway  J Periodontal Res  2015  50(6):855-63  PubMed ID: 25900259   DOI: 10.1111/jre.12277
5992  Divya MS, Roshin GE, Divya TS, Rasheed VA, Santhoshkumar TR, Elizabeth KE, James J, Pillai RM.  Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation.  Stem Cell Res Ther  2012  3(6):57  PubMed ID: 23253356   DOI: 10.1186/scrt148



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