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Cell No. : Cell Name
RCB2079 : UCB-TERT-21  update : 2022/05/10
CommentHuman mesenchymal cell line derived from umbilical cord blood. Immortalized by hTERT.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal human < Mammals
Genus Homo
Species sapiens
Tissue umbilical cord blood
Classification transformed
Recombinant recombinant
Exogene hTERT, puromycin
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3232
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives PLUSOID-M
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2 x 10^(5) cells/100 mm dish
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 0

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3476  Terai M1, Uyama T, Sugiki T, Li XK, Umezawa A, Kiyono T.  Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway.  Mol Biol Cell  2005  16(3):1491-9  PubMed ID: 15647378   DOI: 10.1091/mbc.e04-07-0652

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