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Cell No. : Cell Name
RCB2016 : AMR19  update : 2021/05/20
CommentHybridoma producing a monoclonal antibody against ganglioside lactones.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Tokyo Metropolitan Institute of Medical Science
Department of Molecular Medicine
2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 Japan
Dr.KAWASHIMA Ikuo
Fax. +81-3-5316-3173
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawashima, Ikuo (Tai, Tadashi)
Originator Tai, Tadashi
Year of deposit 2005
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U831
hybridoma Fusion_cell_1 name PAI
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, C3H/HeN
Fusion method PEG, HAT selection
Antibody name AMR19
Subclass IgM
Immunogen GD3 lactone gangliosides
Antigen_character GD3 lactone
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS (heat inactivated)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Reference information Reference 1
User's Publication 0


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Reference
3510  Kawashima I, Kotani M, Ozawa H, Suzuki M, Tai T.  Generation of monoclonal antibodies specific for ganglioside lactones: evidence of the expression of lactone on human melanoma cells.  Int J Cancer  1994  58(2):263-8  PubMed ID: 8026889   DOI: 10.1002/ijc.2910580220

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