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Cell No. : Cell Name
RCB2015 : GMR8  update : 2024/01/17
CommentHybridoma producing a monoclonal antibody against N-glycolylneuraminic acid-containing gangliosides (GM3 gangliosides).
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Tokyo Metropolitan Institute of Medical Science
Department of Molecular Medicine
2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 Japan
Dr.KAWASHIMA Ikuo
Fax. +81-3-5316-3173
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawashima, Ikuo (Tai, Tadashi)
Originator Tai, Tdashi
Year of deposit 2005
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U837
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, C3H/HeN
Fusion_cell_2 name PAI
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name GMR8
Subclass IgM
Immunogen GM3(NeuGc) gangliosides
Antigen_character GM3(NeuGc)
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS (heat inactivated)
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3508  Ozawa H, Kawashima I, Tai T.  Generation of murine monoclonal antibodies specific for N-glycolylneuraminic acid-containing gangliosides.  Arch Biochem Biophys  1992  294(2):427-33  PubMed ID: 1567198   DOI: 10.1016/0003-9861(92)90707-4

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