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Cell No. : Cell Name
RCB1998 : UV·B6-2.1A  update : 2021/05/20
CommentMouse cell line derived from UV-induced skin tumor.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kuribayashi, Kagemasa
Originator Kuribayashi, Kagemasa
Year of deposit 2005
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Tissue skin
Classification transformed
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4E34
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
2946  Kuribayashi K, Tanaka C, Matsubayashi Y, Masuda T, Udono H, Abe M, Nakayama E, Shiku H  Anti-idiotypic antibodies against UV-induced tumor-specific CTL clones. Preparation in syngeneic combination.  J Immunol  1988  141:4074-80  PubMed ID: 2460558  

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