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Cell No. : Cell Name
RCB1994 : UV♀2  update : 2022/03/11
CommentMouse cell line derived from UV-induced angioendothelioma-like tumor.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kuribayashi, Kagemasa
Originator Kuribayashi, Kagemasa
Year of deposit 2005
Another name F-2
Animal mouse < Mammals
Strain name (BALB/c×C57BL/6)(CB6)F1
Gender Female
Tissue vascular endothelial cell
Classification transformed
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U951
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 17


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Reference
2948  Toda K, Tsujioka K, Maruguchi Y, Ishii K, Miyachi Y, Kuribayashi K, Imamura S.  Establishment and characterization of a tumorigenic murine vascular endothelial cell line (F-2).  Cancer Res  1990  50(17):5526-30  PubMed ID: 2386957  

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User's Publication
20376  Nagano F, Mizuno T, Imai M, Takahashi K, Tsuboi N, Maruyama S, Mizuno M.  Expression of a Crry/p65 is reduced in acute lung injury induced by extracellular histones.  FEBS Open Bio  2022    PubMed ID: 34709768   DOI: 10.1002/2211-5463.13322
16642  Gulay KCM, Aoshima K, Maekawa N, Suzuki T, Konnai S, Kobayashi A, Kimura T.  Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages  Sci Rep  2022  12(1):2124  PubMed ID: 35136176   DOI: 10.1038/s41598-022-06203-w
16734  Sasaki S, Oba K, Kodera Y, Itakura M, Shichiri M.  ANGT_HUMAN[448-462], an Anorexigenic Peptide Identified Using Plasma Peptidomics  J Endocr Soc  2022  6(7):bvac082  PubMed ID: 35702602   DOI: 10.1210/jendso/bvac082
10776  Nagano F, Mizuno T, Mizumoto S, Yoshioka K, Takahashi K, Tsuboi N, Maruyama S, Yamada S, Nagamatsu T.  Chondroitin sulfate protects vascular endothelial cells from toxicities of extracellular histones.  Eur. J. Pharmacol.  2018  826:48-55  PubMed ID: 29501579   DOI: 10.1016/j.ejphar.2018.02.043
11073  Obata Y, Kita S, Koyama Y, Fukuda S, Takeda H, Takahashi M, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nakamura Y, Nishizawa H, Funahashi T, Ranscht B, Izumi Y, Bamba T, Fukusaki E, Hanayama R, Shimada S, Maeda N, Shimomura I.  Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.  JCI Insight  2018    PubMed ID: 29669945   DOI: 10.1172/jci.insight.99680
4369  Tsuji-Tamura K, Ogawa M.  Dual inhibition of mTORC1 and mTORC2 perturbs cytoskeletal organization and impairs endothelial cell elongation.  Biochem. Biophys. Res. Commun.  2018  497(1):326-331  PubMed ID: 29428724   DOI: 10.1016/j.bbrc.2018.02.080
9430  Akira Mochizuki, Chie Yahata, Hung Takai  Cytocompatibility of Magnesium and AZ31 Alloy With Three Types of Cell Lines Using a Direct in Vitro Method  J Mater Sci Mater Med  2016  27(9):145  PubMed ID: 27568216   DOI: 10.1007/s10856-016-5762-x
7254  Miura K, Nakaya H, Kobayashi Y.  Experimental assessment of effects of antiproliferative drugs of drug-eluting stents on endothelial cells.  Cardiovasc Revasc Med  2015  16:344-7  PubMed ID: 26253188   DOI: 10.1016/j.carrev.2015.07.002
10192  Hiroaki Yazama, Kazuyuki Kitatani, Kazunori Fujiwara, Misaki Kato, Mayumi Hashimoto-Nishimura, Katsuyuki Kawamoto, Kensaku Hasegawa, Hiroya Kitano, Alicja Bielawska, Jacek Bielawski, Toshiro Okazaki  Dietary Glucosylceramides Suppress Tumor Growth in a Mouse Xenograft Model of Head and Neck Squamous Cell Carcinoma by the Inhibition of Angiogenesis Through an Increase in Ceramide  Int J Clin Oncol  2015  20(3):438-46  PubMed ID: 25080062   DOI: 10.1007/s10147-014-0734-y
15131  Karasawa K, Asano K, Moriyama S, Ushiki M, Monya M, Iida M, Kuboki E, Yagita H, Uchida K, Nitta K, Tanaka M.  Vascular-resident CD169-positive monocytes and macrophages control neutrophil accumulation in the kidney with ischemia-reperfusion injury  J Am Soc Nephrol  2015  26(4):896-906  PubMed ID: 25266072   DOI: 10.1681/ASN.2014020195
15216  Matsuda K, Fujishima Y, Maeda N, Mori T, Hirata A, Sekimoto R, Tsushima Y, Masuda S, Yamaoka M, Inoue K, Nishizawa H, Kita S, Ranscht B, Funahashi T, Shimomura I.  Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice  Endocrinology  2015  156(3):934-46  PubMed ID: 25514086   DOI: 10.1210/en.2014-1618
15191  Hattori Y, Kim H, Tsuboi N, Yamamoto A, Akiyama S, Shi Y, Katsuno T, Kosugi T, Ueda M, Matsuo S, Maruyama S.  Therapeutic Potential of Stem Cells from Human Exfoliated Deciduous Teeth in Models of Acute Kidney Injury  PLoS One  2015  10(10):e0140121  PubMed ID: 26509261   DOI: 10.1371/journal.pone.0140121
12562  Enoki Y, Sato T, Tanaka S, Iwata T, Usui M, Takeda S, Kokabu S, Matsumoto M, Okubo M, Nakashima K, Yamato M, Okano T, Fukuda T, Chida D, Imai Y, Yasuda H, Nishihara T, Akita M, Oda H, Okazaki Y, Suda T, Yoda T.  Netrin-4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo  FEBS Lett  2014  588(14):2262-9  PubMed ID: 24846137   DOI: 10.1016/j.febslet.2014.05.009
15993  Shi Y, Tsuboi N, Furuhashi K, Du Q, Horinouchi A, Maeda K, Kosugi T, Matsuo S, Maruyama S.  Pristane-induced granulocyte recruitment promotes phenotypic conversion of macrophages and protects against diffuse pulmonary hemorrhage in Mac-1 deficiency  J Immunol  2014  193(10):5129-39  PubMed ID: 25281714   DOI: 10.4049/jimmunol.1401051
15661  Fiorcari S, Brown WS, McIntyre BW, Estrov Z, Maffei R, O'Brien S, Sivina M, Hoellenriegel J, Wierda WG, Keating MJ, Ding W, Kay NE, Lannutti BJ, Marasca R, Burger JA.  The PI3-kinase delta inhibitor idelalisib (GS-1101) targets integrin-mediated adhesion of chronic lymphocytic leukemia (CLL) cell to endothelial and marrow stromal cells  PLoS One  2013  8(12):e83830  PubMed ID: 24376763   DOI: 10.1371/journal.pone.0083830
16033  Yazaki Y, Oyane A, Araki H, Sogo Y, Ito A, Yamazaki A, Tsurushima H.  Fabrication of DNA-antibody-apatite composite layers for cell-targeted gene transfer  Sci Technol Adv Mater  2012  13(6):064204  PubMed ID: 27877531   DOI: 10.1088/1468-6996/13/6/064204
7681  Akashi M, Higashi T, Masuda S, Komori T, Furuse M.  A coronary artery disease-associated gene product, JCAD/KIAA1462, is a novel component of endothelial cell-cell junctions.  Biochem. Biophys. Res. Commun.  2011  413:224-9  PubMed ID: 21884682   DOI: 10.1016/j.bbrc.2011.08.073



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