Cell No. : Cell Name
RCB1991 : KUSA-O
update : 2024/08/16
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| Comment | Mouse multipotent mesenchymal cells. Derived from bone marrow. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Umezawa, Akihiro
|
| Year of deposit |
2005
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/He
|
| Gender |
Female
|
| Tissue |
bone marrow
|
| Classification |
normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_5223
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
2 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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|
| Reference information |
Reference |
4
|
| User's Publication |
2
|
| Reference |
|
2514
Kawashima N, Shindo K, Sakamoto K, Kondo H, Umezawa A, Kasugai S, Perbal B, Suda H, Takagi M, Katsube K.
Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa
J Bone Miner Metab
2005
23(2):123-33
PubMed ID: 15750690
DOI: 10.1007/s00774-004-0550-y
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2512
Shindo K, Kawashima N, Sakamoto K, Yamaguchi A, Umezawa A, Takagi M, Katsube K, Suda H.
Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling
Exp Cell Res
2003
290(2):370-80
PubMed ID: 14567994
DOI: 10.1016/s0014-4827(03)00349-5
|
|
2528
Elizabeth H. Allan, Patricia W.M. Ho, Akihro Umezawa, Jun-Ichi Hata, Fusao Makishima, Matthew T. Gillespie, T. John Martin
Differentiation potential of a mouse bone marrow stromal cell line
Journal of Cellular Biochemistry
2003
90:158-169
PubMed ID: 12938165
DOI: 10.1002/jcb.10614
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2753
Umezawa A, Maruyama T, Segawa K, Shadduck RK, Waheed A, Hata J
Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.
J Cell Physiol
1992
151:197-205
PubMed ID: 1373147
DOI: 10.1002/jcp.1041510125
|
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