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Cell No. : Cell Name
RCB1991 : KUSA-O  update : 2023/01/05
CommentMouse multipotent mesenchymal cells. Derived from bone marrow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal _mouse < Mammals
Strain name C3H/He
Gender Female
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5223
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2 x 10 5 cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 2

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2514  Kawashima N, Shindo K, Sakamoto K, Kondo H, Umezawa A, Kasugai S, Perbal B, Suda H, Takagi M, Katsube K.  Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa  J Bone Miner Metab  2005  23(2):123-33  PubMed ID: 15750690   DOI: 10.1007/s00774-004-0550-y
2512  Shindo K, Kawashima N, Sakamoto K, Yamaguchi A, Umezawa A, Takagi M, Katsube K, Suda H.  Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling  Exp Cell Res  2003  290(2):370-80  PubMed ID: 14567994   DOI: 10.1016/s0014-4827(03)00349-5
2528  Elizabeth H. Allan, Patricia W.M. Ho, Akihro Umezawa, Jun-Ichi Hata, Fusao Makishima, Matthew T. Gillespie, T. John Martin  Differentiation potential of a mouse bone marrow stromal cell line  Journal of Cellular Biochemistry  2003  90:158-169  PubMed ID: 12938165   DOI: 10.1002/jcb.10614
2753  Umezawa A, Maruyama T, Segawa K, Shadduck RK, Waheed A, Hata J  Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.  J Cell Physiol  1992  151:197-205  PubMed ID: 1373147   DOI: 10.1002/jcp.1041510125

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User's Publication
9271  Masato Tamura, Maki Uyama, Yuri Sugiyama, Mari Sato  Canonical Wnt Signaling Activates miR-34 Expression During Osteoblastic Differentiation  Mol Med Rep  2013  8(6):1807-11  PubMed ID: 24100761   DOI: 10.3892/mmr.2013.1713
15695  Chaleawlert-Umpon S, Mayen V, Manotham K, Pimpha N.  Preparation of iron oxide-entrapped chitosan nanoparticles for stem cell labeling  J Biomater Sci Polym Ed  2010  21(11):1515-32  PubMed ID: 20537238   DOI: 10.1163/092050609X12519805626031

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