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Cell No. : Cell Name
RCB1981 : HD-Mar2  update : 2022/09/27
CommentA cell line derived from the pleural effusion of a patient with a recurrent T-lymphoblastic lymphoma after treatment of the originally diagnosed Hodgkin’s lymphoma. The characteristics is T cell lymphoma. TKG0198 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0198
Animal human < Mammals
Genus Homo
Species sapiens
Tissue pleural effusion
Disease name Hodgkin’s lymphoma, T cell lymphoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG00198)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2247
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
5072  Ben-Bassat H, Mitrani-Rosenbaum S, Gamliel H, Naparstek E, Leizerowitz R, Korkesh A, Sagi M, Voss R, Kohn G, Polliack A.  Establishment in continuous culture of a T-lymphoid cell line (HD-Mar) from a patient with Hodgkin's lymphoma.  Int J Cancer  1980  25(5):583-90  PubMed ID: 6154663

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