細胞番号 : 細胞名
RCB1980 : CCRF-CEM
update : 2024/08/14
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細胞特性(Comment:英) | Human cell line derived from T cell leukemia. TKG0563 (Deposited from Tohoku Univ.). |
細胞特性(日) | ヒトTリンパ球性白血病由来細胞株。 TKG0563 (東北大学医用細胞資源センターからの寄託)。 |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | |
使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
|
|
寄託者 |
Obinata, Masuo
|
寄託日 |
2004
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元の細胞 |
TKG0563
|
動物種 |
_human
< Mammals
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属名 |
Homo
|
種名 |
sapiens
|
性別 |
Female
|
齢 |
4 years
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採取組織 |
T acute lymphoblastic leukemia
|
細胞分類 |
cancer
|
入手歴 |
Cell Resource Center for Biomedical Research, Tohoku University(TKG0563)
|
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細胞寿命 |
infinite
|
細胞形態 |
lymphocyte-like
|
Cellosaurus(Expasy) |
CVCL_0207
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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培地 |
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RPMI1640 + 10% FBS
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抗生物質 |
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Free
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継代方法 |
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dilution
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継代密度 |
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1 : 10 split
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継代・培地交換頻度 |
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Subculture : 2 times/week
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培養最適温度 |
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37
℃
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二酸化炭素濃度 |
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5
%
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凍結培地 |
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Medium + 10% DMSO
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凍結方法 |
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Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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ウィルス(HIV) |
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LT 400
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ウィルス(HTLV-1) |
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(-)
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アイソザイム検査 |
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LD, NP
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個体識別検査 |
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OK
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文献情報 |
Reference(英) |
8件
|
Reference(日) |
0件
|
利用者成果(英) |
6件
|
利用者成果(日) |
0件
|
Reference(英) |
5071
Drexler HG, Gaedicke G, Minowada J.
T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.
Blut
1987
54(2):79-87
PubMed ID: 3493047
DOI: 10.1007/bf00321034
|
5042
Haruta Y, Seon BK.
Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.
Proc Natl Acad Sci U S A
1986
83(20):7898-902
PubMed ID: 3464004
DOI: 10.1073/pnas.83.20.7898
|
164
Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D.
Correlation of surface markers of cells of human lymphatic leukemias with disease type
J Natl Cancer Inst
1977
59(3):845-50
PubMed ID: 302342
DOI: 10.1093/jnci/59.3.845
|
4745
Pauly JL, Minowada J, Han T, Moore GE.
Disparity of mixed lymphocyte reactivity to cultured cells of human T and B lymphoid lines.
J Natl Cancer Inst
1975
54(3):557-62.
PubMed ID: 123598
|
165
Srivastava BI, Minowada J, Moore GE.
High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin
J Natl Cancer Inst
1975
55(1):11-14
PubMed ID: 808634
DOI: 10.1093/jnci/55.1.11
|
5070
Adams RA, Foley GE, Uzman BG, Farber S, Lazarus S, Kleinman L.
Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.
Cancer Res
1967
27(4):772-83
PubMed ID: 4295047
|
5069
Uzman BG, Foley GE, Farber S, Lazarus H.
Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.
Cancer
1966
19(11):1725-42
PubMed ID: 5224274
DOI: 10.1002/1097-0142(196611)19:11<1725::aid-cncr2820191142>3.0.co;2-t
|
5068
McCarthy RE, Junius V, Farber S, Lazarus H, Foley GE.
Cytogenetic analysis of human lymphoblasts in continuous culture.
Exp Cell Res
1965
40(1):197-200
PubMed ID: 5320250
DOI: 10.1016/0014-4827(65)90316-2
|
利用者成果(英) |
13384
Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.
Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.
Oncol Lett
2021
PubMed ID: 33376545
DOI: 10.3892/ol.2020.12374
|
20013
Iriguchi S, Yasui Y, Kawai Y, Arima S, Kunitomo M, Sato T, Ueda T, Minagawa A, Mishima Y, Yanagawa N, Baba Y, Miyake Y, Nakayama K, Takiguchi M, Shinohara T, Nakatsura T, Yasukawa M, Kassai Y, Hayashi A, Kaneko S.
A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.
Nat Commun
2021
12(1):430
PubMed ID: 33462228
DOI: 10.1038/s41467-020-20658-3
|
20371
Ishihara K, Narita Y, Teramura Y, Fukazawa K.
Preparation of Magnetic Hydrogel Microparticles with Cationic Surfaces and Their Cell-Assembling Performance.
ACS Biomater Sci Eng
2021
PubMed ID: 34677934
DOI: 10.1021/acsbiomaterials.1c01150
|
14826
Nakashima M, Yamochi T, Watanabe M, Uchimaru K, Utsunomiya A, Higashihara M, Watanabe T, Horie R.
CD30 Characterizes Polylobated Lymphocytes and Disease Progression in HTLV-1-Infected Individuals
Clin Cancer Res
2018
24(21):5445-5457
PubMed ID: 30068708
DOI: 10.1158/1078-0432.CCR-18-0268
|
10640
Aoki S, Morita M, Hirao T, Yamaguchi M, Shiratori R, Kikuya M, Chibana H, Ito K.
Shift in energy metabolism caused by glucocorticoids enhances the effect of cytotoxic anti-cancer drugs against acute lymphoblastic leukemia cells.
Oncotarget
2017
8:94271-94285
PubMed ID: 29212227
DOI: 10.18632/oncotarget.21689
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13468
Kawaguchi M, Aoki S, Hirao T, Morita M, Ito K.
Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway
Biochem Biophys Res Commun
2016
474(1):188-192
PubMed ID: 27107693
DOI: 10.1016/j.bbrc.2016.04.098
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