Cell No. : Cell Name
RCB1980 : CCRF-CEM
update : 2021/05/20
|
| Comment | Human cell line derived from T cell leukemia. TKG0563 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Obinata, Masuo
|
| Year of deposit |
2004
|
| Original cell |
TKG0563
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
4 years
|
| Tissue |
T acute lymphoblastic leukemia
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0563）
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_0207
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Medium and additives |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
LT 400
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
6
|
| User's Publication |
6
|
| Reference |
|
5071
Drexler HG, Gaedicke G, Minowada J.
T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.
Blut
1987
54(2):79-87
PubMed ID: 3493047
|
|
5042
Haruta Y, Seon BK.
Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.
Proc Natl Acad Sci U S A
1986
83(20):7898-902
PubMed ID: 3464004
|
|
4745
Pauly JL, Minowada J, Han T, Moore GE.
Disparity of mixed lymphocyte reactivity to cultured cells of human T and B lymphoid lines.
J Natl Cancer Inst
1975
54(3):557-62.
PubMed ID: 123598
|
|
5070
Adams RA, Foley GE, Uzman BG, Farber S, Lazarus S, Kleinman L.
Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.
Cancer Res
1967
27(4):772-83
PubMed ID: 4295047
|
|
5069
Uzman BG, Foley GE, Farber S, Lazarus H.
Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.
Cancer
1966
19(11):1725-42
PubMed ID: 5224274
|
|
5068
McCarthy RE, Junius V, Farber S, Lazarus H, Foley GE.
Cytogenetic analysis of human lymphoblasts in continuous culture.
Exp Cell Res
1965
40(1):197-200
PubMed ID: 5320250
|
| User's Publication |
|
13384
Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.
Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.
Oncol Lett
2021
PubMed ID: 33376545
|
|
20013
Iriguchi S, Yasui Y, Kawai Y, Arima S, Kunitomo M, Sato T, Ueda T, Minagawa A, Mishima Y, Yanagawa N, Baba Y, Miyake Y, Nakayama K, Takiguchi M, Shinohara T, Nakatsura T, Yasukawa M, Kassai Y, Hayashi A, Kaneko S.
A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.
Nat Commun
2021
12(1):430
PubMed ID: 33462228
|
|
20371
Ishihara K, Narita Y, Teramura Y, Fukazawa K.
Preparation of Magnetic Hydrogel Microparticles with Cationic Surfaces and Their Cell-Assembling Performance.
ACS Biomater Sci Eng
2021
PubMed ID: 34677934
|
|
14826
Nakashima M, Yamochi T, Watanabe M, Uchimaru K, Utsunomiya A, Higashihara M, Watanabe T, Horie R.
CD30 Characterizes Polylobated Lymphocytes and Disease Progression in HTLV-1-Infected Individuals
Clin Cancer Res
2018
24(21):5445-5457
PubMed ID: 30068708
|
|
10640
Aoki S, Morita M, Hirao T, Yamaguchi M, Shiratori R, Kikuya M, Chibana H, Ito K.
Shift in energy metabolism caused by glucocorticoids enhances the effect of cytotoxic anti-cancer drugs against acute lymphoblastic leukemia cells.
Oncotarget
2017
8:94271-94285
PubMed ID: 29212227
|
|
13468
Kawaguchi M, Aoki S, Hirao T, Morita M, Ito K.
Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway
Biochem Biophys Res Commun
2016
474(1):188-192
PubMed ID: 27107693
|
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