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Cell No. : Cell Name
RCB1980 : CCRF-CEM  update : 2024/06/12
CommentHuman cell line derived from T cell leukemia. TKG0563 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0563
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 4 years
Tissue T acute lymphoblastic leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0563)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0207
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 8
User's Publication 6

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5071  Drexler HG, Gaedicke G, Minowada J.  T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.  Blut  1987  54(2):79-87  PubMed ID: 3493047   DOI: 10.1007/bf00321034
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
164  Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D.  Correlation of surface markers of cells of human lymphatic leukemias with disease type  J Natl Cancer Inst  1977  59(3):845-50  PubMed ID: 302342   DOI: 10.1093/jnci/59.3.845
4745  Pauly JL, Minowada J, Han T, Moore GE.  Disparity of mixed lymphocyte reactivity to cultured cells of human T and B lymphoid lines.  J Natl Cancer Inst  1975  54(3):557-62.  PubMed ID: 123598  
165  Srivastava BI, Minowada J, Moore GE.  High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin  J Natl Cancer Inst  1975  55(1):11-14  PubMed ID: 808634   DOI: 10.1093/jnci/55.1.11
5070  Adams RA, Foley GE, Uzman BG, Farber S, Lazarus S, Kleinman L.  Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.  Cancer Res  1967  27(4):772-83  PubMed ID: 4295047  
5069  Uzman BG, Foley GE, Farber S, Lazarus H.  Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.  Cancer  1966  19(11):1725-42  PubMed ID: 5224274   DOI: 10.1002/1097-0142(196611)19:11<1725::aid-cncr2820191142>;2-t
5068  McCarthy RE, Junius V, Farber S, Lazarus H, Foley GE.  Cytogenetic analysis of human lymphoblasts in continuous culture.  Exp Cell Res  1965  40(1):197-200  PubMed ID: 5320250   DOI: 10.1016/0014-4827(65)90316-2

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User's Publication
13384  Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.  Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.  Oncol Lett  2021    PubMed ID: 33376545   DOI: 10.3892/ol.2020.12374
20013  Iriguchi S, Yasui Y, Kawai Y, Arima S, Kunitomo M, Sato T, Ueda T, Minagawa A, Mishima Y, Yanagawa N, Baba Y, Miyake Y, Nakayama K, Takiguchi M, Shinohara T, Nakatsura T, Yasukawa M, Kassai Y, Hayashi A, Kaneko S.  A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.  Nat Commun  2021  12(1):430  PubMed ID: 33462228   DOI: 10.1038/s41467-020-20658-3
20371  Ishihara K, Narita Y, Teramura Y, Fukazawa K.  Preparation of Magnetic Hydrogel Microparticles with Cationic Surfaces and Their Cell-Assembling Performance.  ACS Biomater Sci Eng  2021    PubMed ID: 34677934   DOI: 10.1021/acsbiomaterials.1c01150
14826  Nakashima M, Yamochi T, Watanabe M, Uchimaru K, Utsunomiya A, Higashihara M, Watanabe T, Horie R.  CD30 Characterizes Polylobated Lymphocytes and Disease Progression in HTLV-1-Infected Individuals  Clin Cancer Res  2018  24(21):5445-5457  PubMed ID: 30068708   DOI: 10.1158/1078-0432.CCR-18-0268
10640  Aoki S, Morita M, Hirao T, Yamaguchi M, Shiratori R, Kikuya M, Chibana H, Ito K.  Shift in energy metabolism caused by glucocorticoids enhances the effect of cytotoxic anti-cancer drugs against acute lymphoblastic leukemia cells.  Oncotarget  2017  8:94271-94285  PubMed ID: 29212227   DOI: 10.18632/oncotarget.21689
13468  Kawaguchi M, Aoki S, Hirao T, Morita M, Ito K.  Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway  Biochem Biophys Res Commun  2016  474(1):188-192  PubMed ID: 27107693   DOI: 10.1016/j.bbrc.2016.04.098

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