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Cell No. : Cell Name
RCB1974 : SAS  update : 2020/09/24
CommentHuman cell line derived from tongue cancer. Squamous cell carcinoma. TKG0470 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Takahashi, K.
Year of deposit 2004
Original cell TKG0470
Animal human < Mammals
Genus Homo
Species sapiens
Tissue tongue
Case history squamous cell carcinoma of tongue
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0470)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 58

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5067  Yoshida H, Hasegawa S, Kuromi H, Inagaki N, Seino S, Takahashi K, Sato K.  A variant form of laminin is responsible for the neurite outgrowth-promoting activity in conditioned medium from a squamous carcinoma cell line.  Connect Tissue Res  1993  30(1):23-35.  PubMed ID: 8149742

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User's Publication
11965  Fujii S, Tajiri Y, Hasegawa K, Matsumoto S, Yoshimoto RU, Wada H, Kishida S, Kido MA, Yoshikawa H, Ozeki S, Kiyoshima T.  The TRPV4-AKT axis promotes oral squamous cell carcinoma cell proliferation via CaMKII activation.  Lab. Invest.  2020    PubMed ID: 31857698
12037  Nakagawa F, Higashi S, Ando E, Ohsumi T, Watanabe S, Takeuchi H.  Modification of TRPV4 activity by acetaminophen.  Heliyon  2020    PubMed ID: 32051870
12128  Hazawa M, Yoshino H, Nakagawa Y, Shimizume R, Nitta K, Sato Y, Sato M, Wong RW, Kashiwakura I.  Karyopherin-β1 Regulates Radioresistance and Radiation-Increased Programmed Death-Ligand 1 Expression in Human Head and Neck Squamous Cell Carcinoma Cell Lines.  Cancers (Basel)  2020    PubMed ID: 32276424
11354  Nakamura Y, Nakahata S, Kondo Y, Izumi A, Yamamoto K, Ichikawa T, Tamura T, Noumi K, Yamashita Y, Morishita K.  Overexpression of absent in melanoma 2 in oral squamous cell carcinoma contributes to tumor progression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30587341
11377  Inoue K, Hatano K, Hanamatsu Y, Saigo C, Kito Y, Bunai K, Shibata T, Takeuchi T.  Pathobiological role of cleft palate transmembrane protein 1 family proteins in oral squamous cell carcinoma.  J. Cancer Res. Clin. Oncol.  2019    PubMed ID: 30635792
11405  Uzawa K, Kasamatsu A, Saito T, Kita A, Sawai Y, Toeda Y, Koike K, Nakashima D, Endo Y, Shiiba M, Takiguchi Y, Tanzawa H.  Growth suppression of human oral cancer cells by candidate agents for cetuximab-side effects.  Exp. Cell Res.  2019    PubMed ID: 30690028
11497  Yasukochi A, Kawakubo-Yasukochi T, Morioka M, Hazekawa M, Nishinakagawa T, Ono K, Nakashima M, Nakamura S.  Regulation of collagen type XVII expression by miR203a-3p in oral squamous cell carcinoma cells.  J. Biochem.  2019    PubMed ID: 30918974
11616  Hano K, Hatano K, Saigo C, Kito Y, Shibata T, Takeuchi T.  An adiponectin paralog protein, CTRP6 decreased the proliferation and invasion activity of oral squamous cell carcinoma cells: possible interaction with laminin receptor pathway.  Mol. Biol. Rep.  2019    PubMed ID: 31254244
11759  Uzawa K, Amelio AL, Kasamatsu A, Saito T, Kita A, Fukamachi M, Sawai Y, Toeda Y, Eizuka K, Hayashi F, Kato-Kase I, Sunohara M, Iyoda M, Koike K, Nakashima D, Ogawara K, Endo-Sakamoto Y, Shiiba M, Takiguchi Y, Yamauchi M, Tanzawa H.  Resveratrol Targets Urokinase-Type Plasminogen Activator Receptor Expression to Overcome Cetuximab-Resistance in Oral Squamous Cell Carcinoma.  Sci Rep  2019    PubMed ID: 31434965
11775  Hazekawa M, Nishinakagawa T, Kawakubo-Yasukochi T, Nakashima M.  Evaluation of IC50 levels immediately after treatment with anticancer reagents using a real-time cell monitoring device.  Exp Ther Med  2019    PubMed ID: 31555392
9696  Kazuya Hiroshima, Masashi Shiiba, Noritoshi Oka, Fumihiko Hayashi, Sho Ishida, Reo Fukushima, Kazuyuki Koike, Manabu Iyoda, Dai Nakashima, Hideki Tanzawa, Katsuhiro Uzawa  Tspan15 Plays a Crucial Role in Metastasis in Oral Squamous Cell Carcinoma  Exp Cell Res  2019  384(2):111622  PubMed ID: 31518558
9890  Noritoshi Oka, Atsushi Kasamatsu, Yosuke Endo-Sakamoto, Keitaro Eizuka, Sho Wagai, Nao Koide-Ishida, Isao Miyamoto, Manabu Iyoda, Hideki Tanzawa, Katsuhiro Uzawa  Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement  J Cancer  2019  10(16):3728-3734  PubMed ID: 31333790
11921  Okada R, Koshizuka K, Yamada Y, Moriya S, Kikkawa N, Kinoshita T, Hanazawa T, Seki N.  Regulation of Oncogenic Targets by miR-99a-3p (Passenger Strand of miR-99a-Duplex) in Head and Neck Squamous Cell Carcinoma.  Cells  2019    PubMed ID: 31795200
10549  Adachi Y, Yoshimura M, Nishida K, Usuki H, Shibata K, Hattori M, Kondo N, Yatabe Y, Iwata H, Kikumori T, Kodera Y, Nakanishi H.  Acute phase dynamics of circulating tumor cells after paclitaxel and doxorubicin chemotherapy in breast cancer mouse models.  Breast Cancer Res. Treat.  2018  167:439-450  PubMed ID: 29027049
10623  Bunai K, Okubo H, Hano K, Inoue K, Kito Y, Saigo C, Shibata T, Takeuchi T.  TMEM207 hinders the tumour suppressor function of WWOX in oral squamous cell carcinoma.  J. Cell. Mol. Med.  2018  22:1026-1033  PubMed ID: 29164763
10649  Koshizuka K, Hanazawa T, Kikkawa N, Katada K, Okato A, Arai T, Idichi T, Osako Y, Okamoto Y, Seki N.  Antitumor miR-150-5p and miR-150-3p inhibit cancer cell aggressiveness by targeting SPOCK1 in head and neck squamous cell carcinoma.  Auris Nasus Larynx  2018  45:854-865  PubMed ID: 29233721
10664  Ohnishi Y, Yasui H, Nozaki M, Nakajima M.  Molecularly-targeted therapy for the oral cancer stem cells.  Jpn Dent Sci Rev  2018  54:88-103  PubMed ID: 29755619
10682  Yamamoto JI, Kasamatsu A, Okubo Y, Nakashima D, Fushimi K, Minakawa Y, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Evaluation of tryptophan-aspartic acid repeat-containing protein 34 as a novel tumor-suppressor molecule in human oral cancer.  Biochem. Biophys. Res. Commun.  2018  495:2469-2474  PubMed ID: 29278705
10806  Okubo Y, Kasamatsu A, Yamatoji M, Fushimi K, Ishigami T, Shimizu T, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.  Exp. Cell Res.  2018  367:112-118  PubMed ID: 29614312
10820  Fukamachi M, Kasamatsu A, Endo-Sakamoto Y, Fushimi K, Kasama H, Iyoda M, Minakawa Y, Shiiba M, Tanzawa H, Uzawa K.  Multiple coagulation factor deficiency protein 2 as a crucial component in metastasis of human oral cancer.  Exp. Cell Res.  2018  368:119-125  PubMed ID: 29679592
11226  Fukushima R, Kasamatsu A, Nakashima D, Higo M, Fushimi K, Kasama H, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of Translocation Associated Membrane Protein 2 Leading to Cancer-Associated Matrix Metalloproteinase Activation as a Putative Metastatic Factor for Human Oral Cancer.  J Cancer  2018    PubMed ID: 30271493
11306  Kakuguchi W, Nomura T, Kitamura T, Otsuguro S, Matsushita K, Sakaitani M, Maenaka K, Tei K.  Suramin, screened from an approved drug library, inhibits HuR functions and attenuates malignant phenotype of oral cancer cells.  Cancer Med  2018    PubMed ID: 30449075
4236  Nanbu T, Umemura N, Ohkoshi E, Nanbu K, Sakagami H, Shimada J.  Combined SN-38 and gefitinib treatment promotes CD44 degradation in head and neck squamous cell carcinoma cells.  Oncol. Rep.  2018    PubMed ID: 29192320
4237  Yamanouchi R, Harada K, Ferdous T, Ueyama Y.  Low carbonyl reductase 1 expression is associated with poor prognosis in patients with oral squamous cell carcinoma.  Mol Clin Oncol  2018    PubMed ID: 29456845
4345  Hou B, Ishinaga H, Midorikawa K, Nakamura S, Hiraku Y, Oikawa S, Ma N, Takeuchi K, Murata M.  Let-7c inhibits migration and epithelial-mesenchymal transition in head and neck squamous cell carcinoma by targeting IGF1R and HMGA2.  Oncotarget  2018    PubMed ID: 29507664
10148  Takahara T, Kasamatsu A, Yamatoji M, Iyoda M, Kasama H, Saito T, Takeuchi S, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  SIPA1 promotes invasion and migration in human oral squamous cell carcinoma by ITGB1 and MMP7.  Exp. Cell Res.  2017  352:357-363  PubMed ID: 28237246
10155  Koide N, Kasamatsu A, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Evidence for Critical Role of Lymphocyte Cytosolic Protein 1 in Oral Cancer.  Sci Rep  2017  7:43379  PubMed ID: 28230172
10191  Takeuchi S, Kasamatsu A, Yamatoji M, Nakashima D, Endo-Sakamoto Y, Koide N, Takahara T, Shimizu T, Iyoda M, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  TEAD4-YAP interaction regulates tumoral growth by controlling cell-cycle arrest at the G1 phase.  Biochem. Biophys. Res. Commun.  2017  486:385-390  PubMed ID: 28315328
10395  Takada H, Ibaragi S, Eguchi T, Okui T, Obata K, Masui M, Morisawa A, Takabatake K, Kawai H, Yoshioka N, Hassan NMM, Shimo T, Hu GF, Nagatsuka H, Sasaki A.  Semaphorin 4D promotes bone invasion in head and neck squamous cell carcinoma.  Int. J. Oncol.  2017  51:625-632  PubMed ID: 28656278
10418  Kita A, Kasamatsu A, Nakashima D, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis.  J Cancer  2017  8:2033-2041  PubMed ID: 28819404
10929    Assessment of Cytotoxicity of Imatinib for Oral Squamous Cell Carcinoma by a Real-Time Cell Analysis System.  Electronic Journal of Biology  2017  13: 56-62 
4555  Furukawa Y, Takasu A, Yura Y.  Role of autophagy in oncolytic herpes simplex virus type 1-induced cell death in squamous cell carcinoma cells.  Cancer Gene Ther.  2017  24(9):393-400  PubMed ID: 28984290
4574  Yasui H, Ohnishi Y, Nakajima M, Nozaki M.  Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation.  Oncol Rep  2017  37(6):3674-3680  PubMed ID: 28440510
6024  Hamada M, Kameyama H, Iwai S, Yura Y.  Induction of autophagy by sphingosine kinase 1 inhibitor PF-543 in head and neck squamous cell carcinoma cells.  Cell Death Discov  2017  3:17047  PubMed ID: 29109864
6027  lNorihisa Ichimura et al  Notch3 is frequently downregulated in oral cancer  Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   2017  29:504-510 
6029  Ishida S et al  Novel mechanism of aberrant ZIP4 expression with zinc supplementation in oral tumorigenesis.  Biochem Biophys Res Commun  2017  483(1):339-345  PubMed ID: 28017725
9616  Kitajima D, Kasamatsu A, Nakashima D, Miyamoto I, Kimura Y, Saito T, Suzuki T, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Tie2 Regulates Tumor Metastasis of Oral Squamous Cell Carcinomas.  J Cancer  2016  7:600-7  PubMed ID: 27053959
9630  Kimura Y, Kasamatsu A, Nakashima D, Yamatoji M, Minakawa Y, Koike K, Fushimi K, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  ARNT2 Regulates Tumoral Growth in Oral Squamous Cell Carcinoma.  J Cancer  2016  7:702-10  PubMed ID: 27076852
9762  Tachibana H, Sho R, Takeda Y, Zhang X, Yoshida Y, Narimatsu H, Otani K, Ishikawa S, Fukao A, Asao H, Iino M.  Circulating miR-223 in Oral Cancer: Its Potential as a Novel Diagnostic Biomarker and Therapeutic Target.  PLoS ONE  2016  11:e0159693  PubMed ID: 27441818
9851  Masui A, Hamada M, Kameyama H, Wakabayashi K, Takasu A, Imai T, Iwai S, Yura Y.  Autophagy as a Survival Mechanism for Squamous Cell Carcinoma Cells in Endonuclease G-Mediated Apoptosis.  PLoS ONE  2016  11:e0162786  PubMed ID: 27658240
9900  Harada K, Ferdous T, Ueyama Y.  Gimeracil Exerts Radiosensitizing Effects on Oral Squamous Cell Carcinoma Cells In Vitro and In Vivo.  Anticancer Res.  2016  36:5923-5930  PubMed ID: 27793917
6036  Suzuki T, Kasamatsu A, Miyamoto I, Saito T, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of TMOD1 is associated with enhanced regional lymph node metastasis in human oral cancer.  Int J Oncol.  2016  48(2):607-12  PubMed ID: 26718916
6042  Unozawa M, Kasamatsu A, Higo M, Fukumoto C, Koyama T, Sakazume T, Nakashima D, Ogawara K, Yokoe H, Shiiba M, Tanzawa H, Uzawa  Cavin-2 in oral cancer: A potential predictor for tumor progression.  Mol Carcinog  2016  55(6):1037-47  PubMed ID: 26086332
7175  Ohnishi Y, Yasui H, Kakudo K, Nozaki M.  Cetuximab-resistant oral squamous cell carcinoma cells become sensitive in anchorage-independent culture conditions through the activation of the EGFR/AKT pathway.  Int. J. Oncol.  2015  47:2165-72  PubMed ID: 26497980
7532  IIKAWA N, YAMAMOTO Y, KAWASAKI Y, YOSHIOKA T, NISHIJIMA A, ENOMOTO K  Intra-Golgi Connexin26 behaves in a pro-oncogenic manner in head and neck cancer cells.  Akita J Med  2015  42:87-94 
6044  Kasama H et al   Adenosine A2b receptor promotes progression of human oral cancer.  BMC Cancer  2015  15:563  PubMed ID: 26228921
6052  Saito T et al  Semaphorin7A Promotion of Tumoral Growth and Metastasis in Human Oral Cancer by Regulation of G1 Cell Cycle and Matrix Metalloproteases: Possible Contribution to Tumoral Angiogenesis.  PLoS One  2015  10(9):e0137923  PubMed ID: 26378920
6053  Miyamoto I et al  Kinesin family member 14 in human oral cancer: A potential biomarker for tumoral growth.  Biochem Biophys Rep  2015  3:26-31  PubMed ID: 29124166
6003  Aoyama 1, Ota Y, Kajiwara K, Hirayama N, Kimura M.  Frequent mutations in NOTCH1 ligand-binding regions in Japanese oral squamous cell carcinoma.  Biochem Biophys Res Commun  2014  452(4):980-5  PubMed ID: 25234595
6023  Watanabe M, Ohnishi Y, Inoue H, Wato M, Tanaka A, Kakudo K, Nozaki M.  NANOG expression correlates with differentiation, metastasis and resistance to preoperative adjuvant therapy in oral squamous cell carcinoma.  Oncol Lett  2014  7(1):35-40  PubMed ID: 24348816
6006  Ohnishi Y, Watanabe M, Yasui H, Kakudo K.  Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines.  Oncol Lett  2014  7(5):1439-1442  PubMed ID: 24765152
5057  Tsujikawa T, Yaguchi T, Ohmura G, Ohta S, Kobayashi A, Kawamura N, Fujita T, Nakano H, Shimada T, Takahashi T, Nakao R, Yanagisawa A, Hisa Y, Kawakami Y.  Autocrine and paracrine loops between cancer cells and macrophages promote lymph node metastasis via CCR4/CCL22 in head and neck squamous cell carcinoma.  Int J Cancer.  2013  132(12):2755-66  PubMed ID: 23180648
6077  Mikami T, Hada T, Chosa N, Ishisaki A, Mizuki H, Takeda Y. Author information  Expression of Wilms' tumor 1 (WT1) in oral squamous cell carcinoma.  J Oral Pathol Med  2013  42(2):133-9  PubMed ID: 22672247
6109  Inoue H, Ohnishi Y, Nakajima M, Kakudo K, Nozaki M.  A novel function of EpCAM in oral squamous cell carcinoma cells under anchorage-independent conditions.  Int J Oncol  2011  39(6):1401-5  PubMed ID: 21785818
6111  Hiroshi Inoue, Yuichi Ohnishi, Masahiro Watanabe, Tomoko Fujii, Yuki Minamino, Yoshihide Ugaki, Suguru Dateoka, Mitsuchika Sugitatsu, Masayuki Tsunokuma, Hirohito Kubo, Masahiro Nakajima, Kenji Kakudo  Effect of EpCAM localization on proliferation of an oral squamous cell carcinoma cell line  Journal of Osaka Dental UniversityJournal of Osaka Dental University  2011  45:259-264 
6112  lHiroshi Inoue et al  Effects of a gamma secretase inhibitor on the proliferation and invasiveness of oral squamous cell carcinoma cell lines  Asian Journal of Oral and Maxillofacial Surgery   2011  23:1-6 
6118  Suguru Dateoka, Yuichi Ohnishi, Kenji Kakudo  Synergistic anti-tumor effect of CDDP with CRM197, a specific inhibitor of HB-EGF, in oral cancer  Journal of Osaka Dental University  2011  45:31-36 
6122  Nomi N, Kodama S, Suzuki M.  Toll-like receptor 3 signaling induces apoptosis in human head and neck cancer via survivin associated pathway.  Oncol Rep  2010  24(1):225-31  PubMed ID: 20514466

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