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Cell No. : Cell Name
RCB1972 : PK-1  update : 2021/11/18
CommentHuman pancreatic carcinoma cell line. RCB2138 was derived from the same patient. TKG0239 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Kobari, M.
Year of deposit 2004
Original cell TKG0239
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue pancreas
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0239)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4717
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 28

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3302  Shibata K, Mori M, Tanaka S, Kitano S, Akiyoshi T  Identification and cloning of human G-protein gamma 7, down-regulated in pancreatic cancer.  Biochem Biophys Res Commun  1998  246:205-9  PubMed ID: 9600093
3301  Yamada T, Okajima F, Ohwada S, Kondo Y  Growth inhibition of human pancreatic cancer cells by sphingosylphosphorylcholine and influence of culture conditions.  Cell Mol Life Sci  1997  53:435-41  PubMed ID: 9176562
5062  Kimura Y, Kobari M, Yusa T, Sunamura M, Kimura M, Shimamura H, Matsuno S.  Establishment of an experimental liver metastasis model by intraportal injection of a newly derived human pancreatic cancer cell line (KLM-1).  Int J Pancreatol  1996  20(1):43-50  PubMed ID: 8872523
5253  Kobari M, Hisano H, Matsuno S, Sato T, Kan M, Tachibana T.  Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.  Tohoku J Exp Med  1986  150(3):231-48  PubMed ID: 3547771
3299  Kobari M, Matsuno S, Sato T, Kan M, Tachibana T  Establishment of a human pancreatic cancer cell line and detection of pancreatic cancer associated antigen.  Tohoku J Exp Med  1984  143:33-46  PubMed ID: 6205469

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User's Publication
20431  Shiozaki A, Konishi T, Kosuga T, Kudou M, Kurashima K, Inoue H, Shoda K, Arita T, Konishi H, Morimura R, Komatsu S, Ikoma H, Toma A, Kubota T, Fujiwara H, Okamoto K, Otsuji E.  Roles of voltage‑gated potassium channels in the maintenance of pancreatic cancer stem cells.  Int J Oncol  2021  59(4)  PubMed ID: 34414448
11820  Okazaki J, Tanahashi T, Sato Y, Miyoshi J, Nakagawa T, Kimura T, Miyamoto H, Fujino Y, Nakamura F, Takehara M, Ma B, Bando M, Kitamura S, Okamoto K, Muguruma N, Sogabe M, Takayama T.  MicroRNA-296-5p Promotes Cell Invasion and Drug Resistance by Targeting Bcl2-Related Ovarian Killer, Leading to a Poor Prognosis in Pancreatic Cancer.  Digestion  2020  101(6):794-806  PubMed ID: 31563901
13542  Takehara M, Sato Y, Kimura T, Noda K, Miyamoto H, Fujino Y, Miyoshi J, Nakamura F, Wada H, Bando Y, Ikemoto T, Shimada M, Muguruma N, Takayama T.  Cancer-associated adipocytes promote pancreatic cancer progression through SAA1 expression  Cancer Sci  2020  111(8):2883-2894  PubMed ID: 32535957
14280  Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D, Campbell SL, Petricoin EF 3rd, Simanshu DK, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.  Atypical KRAS G12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer  Cancer Discov  2020  10(1):104-123  PubMed ID: 31649109
14803  Kushibiki T, Nakamura T, Tsuda M, Tsuchikawa T, Hontani K, Inoko K, Takahashi M, Asano T, Okamura K, Murakami S, Kurashima Y, Ebihara Y, Noji T, Nakanishi Y, Tanaka K, Maishi N, Sasaki K, Park WR, Shichinohe T, Hida K, Tanaka S, Hirano S.  Role of Dimerized C16orf74 in Aggressive Pancreatic Cancer: A Novel Therapeutic Target  Mol Cancer Ther  2020  19(1):187-198  PubMed ID: 31597713
11811  Mohammad GH, Vassileva V, Acedo P, Olde Damink SWM, Malago M, Dhar DK, Pereira SP.  Targeting Pyruvate Kinase M2 and Lactate Dehydrogenase A Is an Effective Combination Strategy for the Treatment of Pancreatic Cancer.  Cancers (Basel)  2019  11(9):1372  PubMed ID: 31527446
9183  Maria New, Tim Van Acker Jun-Ichi Sakamaki, Ming Jiang, Rebecca E Saunders, Jaclyn Long, Victoria M-Y Wang, Axel Behrens, Joana Cerveira, Padhmanand Sudhakar, Tamas Korcsmaros, Harold B J Jefferies, Kevin M Ryan, Michael Howell, Sharon A Tooze  MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma  Cancer Res  2019  79 (8), 1884-1898  PubMed ID: 30765601
10602  Okura R, Fujihara S, Iwama H, Morishita A, Chiyo T, Watanabe M, Hirose K, Kobayashi K, Fujimori T, Kato K, Kamada H, Kobara H, Mori H, Niki T, Hirashima M, Okano K, Suzuki Y, Masaki T.  MicroRNA profiles during galectin-9-induced apoptosis of pancreatic cancer cells.  Oncol Lett  2018  15:407-414  PubMed ID: 29387226
10466  Machihara K, Tanaka H, Hayashi Y, Murakami I, Namba T.  Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78.  Biochem. Biophys. Res. Commun.  2017  492:33-40  PubMed ID: 28811106
13746  Moriya C, Taniguchi H, Miyata K, Nishiyama N, Kataoka K, Imai K.  Inhibition of PRDM14 expression in pancreatic cancer suppresses cancer stem-like properties and liver metastasis in mice  Carcinogenesis  2017  38(6):638-648  PubMed ID: 28498896
14741  Sakamaki JI, Wilkinson S, Hahn M, Tasdemir N, O'Prey J, Clark W, Hedley A, Nixon C, Long JS, New M, Van Acker T, Tooze SA, Lowe SW, Dikic I, Ryan KM.  Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function  Mol Cell   2017  66(4):517-532  PubMed ID: 28525743
9592  Hijiya N, Tsukamoto Y, Nakada C, Tung Nguyen L, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.  Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma.  Cancer Res.  2016  76:2612-25  PubMed ID: 26941286
9735  Ellebaek S, Brix S, Grandal M, Lantto J, Horak ID, Kragh M, Poulsen TT.  Pan-HER-An antibody mixture targeting EGFR, HER2 and HER3 abrogates preformed and ligand-induced EGFR homo- and heterodimers.  Int. J. Cancer  2016  139:2095-105  PubMed ID: 27342948
12659  Mohammad GH, Olde Damink SW, Malago M, Dhar DK, Pereira SP.  Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome  PLoS One  2016  11(3):e0151635  PubMed ID: 26989901
7168  Miyamae M, Komatsu S, Ichikawa D, Kawaguchi T, Hirajima S, Okajima W, Ohashi T, Imamura T, Konishi H, Shiozaki A, Morimura R, Ikoma H, Ochiai T, Okamoto K, Taniguchi H, Otsuji E.  Plasma microRNA profiles: identification of miR-744 as a novel diagnostic and prognostic biomarker in pancreatic cancer.  Br. J. Cancer  2015  113:1467-76  PubMed ID: 26505678
7300  Namba T, Kodama R, Moritomo S, Hoshino T, Mizushima T.  Zidovudine, an anti-viral drug, resensitizes gemcitabine-resistant pancreatic cancer cells to gemcitabine by inhibition of the Akt-GSK3β-Snail pathway.  Cell Death Dis  2015  6:e1795  PubMed ID: 26111057
7362  Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S.  Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.  Cancer Res.  2015  75:2629-40  PubMed ID: 25952647
5019  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  SU11274 suppresses proliferation and motility of pancreatic cancer cells.  Oncol Lett  2015  10(3):1468-1472  PubMed ID: 26622692
15259  Namba T, Kodama R.  Avarol induces apoptosis in pancreatic ductal adenocarcinoma cells by activating PERK-eIF2α-CHOP signaling  Mar Drugs  2015  13(4):2376-89  PubMed ID: 25894488
5018  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Frizzled-2: A potential novel target for molecular pancreatic cancer therapy.  Oncol Lett  2014  7(1):74-78  PubMed ID: 24348824
5024  Kawaguchi T, Komatsu S, Ichikawa D, Morimura R, Tsujiura M, Konishi H, Takeshita H, Nagata H, Arita T, Hirajima S, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.  Br J Cancer  2013  108(2):361-9  PubMed ID: 23329235
3714  Kato N, Yamamoto H, Adachi Y, Ohashi H, Taniguchi H, Suzuki H, Nakazawa M, Kaneto H, Sasaki S, Imai K, Shinomura Y.  Cancer detection by ubiquitin carboxyl-terminal esterase L1 methylation in pancreatobiliary fluids.  World J Gastroenterol  2013  19(11):1718-27  PubMed ID: 23555160
5020  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  nsulin-like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media.  J Cell Biochem  2012  113(8):2714-20  PubMed ID: 22441995
12860  Trono JD, Mizuno K, Yusa N, Matsukawa T, Yokoyama K, Uesaka M.  Size, concentration and incubation time dependence of gold nanoparticle uptake into pancreas cancer cells and its future application to X-Ray Drug Delivery System  J Radiat Res  2011  52(1):103-9.  PubMed ID: 21187668
8294  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer.  World J. Gastroenterol.  2010  16:1854-8  PubMed ID: 20397262
6434  K. MIZUNO, M. UESAKA, S. MATSUYAMA, Y. ITO, K. ISHII and H. YAMAZAKI  Evaluation of the uptake of CDDP-containing polymeric micelles in single pancreatic cancer cells  International Journal of PIXE  2010  20:37-43 
8610  Suzuki J, Murakami Y, Samejima K, Ohtani KK, Oka T.  Antizyme is necessary for conversion of pancreatic tumor cells into glucagon-producing differentiated cells.  Endocr. Relat. Cancer  2009  16:649-59  PubMed ID: 19349429
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309

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