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Cell No. : Cell Name
RCB1970 : LK-2  update : 2024/10/08
CommentHuman lung squamous cell carcinoma cell line. TKG0494 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Yoshioka, S. & Yamane. M.
Year of deposit 2004
Original cell TKG0494
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 74 years
Tissue lung
Disease name squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0494)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1377
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 22


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Reference
3297  Yoshioka, S.  Studies on thiol protease inhibitor isolated from human lung cancer cell line  Hiroshima J Med Sci  1989  37:199-215   

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User's Publication
19673  Paik PK, Fan PD, Qeriqi B, Namakydoust A, Daly B, Ahn L, Kim R, Plodkowski A, Ni A, Chang J, Fanaroff R, Ladanyi M, de Stanchina E, Rudin CM.  Targeting NFE2L2/KEAP1 Mutations in Advanced NSCLC With the TORC1/2 Inhibitor TAK-228  J Thorac Oncol  2023  18(4):516-526  PubMed ID: 36240971   DOI: 10.1016/j.jtho.2022.09.225
19725  Kotani N, Araki T, Miyagawa-Yamaguchi A, Amimoto T, Nakano M, Honke K.  Proximity Labeling and Proteomics: Get to Know Neighbors  Methods Enzymol  2023  131-162  PubMed ID: 36682860   DOI: 10.1016/bs.mie.2022.07.031
17825  Akhela Umapathi, Harishkumar Madhyastha, P.N. Navya, Mandeep Singh, Radha Madhyastha, Hemant Kumar Daima  Surface chemistry driven selective anticancer potential of functional silver nanoparticles toward lung cancer cells  Colloids and Surfaces A: Physicochemical and Engineering Aspects  2022  652:129809    DOI: 10.1016/j.colsurfa.2022.129809
16830  Sumiya R, Terayama M, Hagiwara T, Nakata K, Sekihara K, Nagasaka S, Miyazaki H, Igari T, Yamada K, Kawamura YI.  Loss of GSTO2 contributes to cell growth and mitochondria function via the p38 signaling in lung squamous cell carcinoma  Cancer Sci  2022  113(1):195-204  PubMed ID: 34726807   DOI: 10.1111/cas.15189
13332  Hase H, Aoki M, Matsumoto K, Nakai S, Nagata T, Takeda A, Ueda K, Minami K, Kitae K, Jingushi K, Ueda Y, Yamamoto M, Furukawa T, Sato M, Tsujikawa K.  Cancer type‑SLCO1B3 promotes epithelial‑mesenchymal transition resulting in the tumour progression of non‑small cell lung cancer.  Oncol Rep  2021    PubMed ID: 33155667   DOI: 10.3892/or.2020.7839
20043  Yuan G, Flores NM, Hausmann S, Lofgren SM, Kharchenko V, Angulo-Ibanez M, Sengupta D, Lu X, Czaban I, Azhibek D, Vicent S, Fischle W, Jaremko M, Fang B, Wistuba II, Chua KF, Roth JA, Minna JD, Shao NY, Jaremko Ł, Mazur PK, Gozani O.  Elevated NSD3 histone methylation activity drives squamous cell lung cancer.  Nature  2021  590(7846):504-508  PubMed ID: 33536620   DOI: 10.1038/s41586-020-03170-y
20104  Abe K, Kanehira M, Ohkouchi S, Kumata S, Suzuki Y, Oishi H, Noda M, Sakurada A, Miyauchi E, Fujiwara T, Harigae H, Okada Y.  Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer.  Cancer Med  2021    PubMed ID: 33826244   DOI: 10.1002/cam4.3852
14789  Chang A, Liu L, Ashby JM, Wu D, Chen Y, O'Neill SS, Huang S, Wang J, Wang G, Cheng D, Tan X, Petty WJ, Pasche BC, Xiang R, Zhang W, Sun P.  Recruitment of KMT2C/MLL3 to DNA Damage Sites Mediates DNA Damage Responses and Regulates PARP Inhibitor Sensitivity in Cancer  Cancer Res  2021  81(12):3358-3373  PubMed ID: 33853832   DOI: 10.1158/0008-5472.CAN-21-0688
16801  Minegishi K, Dobashi Y, Tsubochi H, Hagiwara K, Ishibashi Y, Nomura S, Nakamura R, Ohmoto Y, Endo S.  TFF-1 Functions to Suppress Multiple Phenotypes Associated with Lung Cancer Progression  Onco Targets Ther  2021  14:4761-4777  PubMed ID: 34531663   DOI: 10.2147/OTT.S322697
13573  Hisako Kaneda et al  Bimolecule detection for Extracellular Vesicle Screening  bioRxiv  2020  September 27    DOI: 10.1101/2020.07.23.217018
13773  Saigusa D, Motoike IN, Saito S, Zorzi M, Aoki Y, Kitamura H, Suzuki M, Katsuoka F, Ishii H, Kinoshita K, Motohashi H, Yamamoto M.  Impacts of NRF2 activation in non-small-cell lung cancer cell lines on extracellular metabolites  Cancer Sci  2020  111(2):667-678  PubMed ID: 31828882   DOI: 10.1111/cas.14278
11422  Hori R, Yamaguchi K, Sato H, Watanabe M, Tsutsumi K, Iwamoto S, Abe M, Onodera H, Nakamura S, Nakai R.  The discovery and characterization of K-563, a novel inhibitor of the Keap1/Nrf2 pathway produced by Streptomyces sp.  Cancer Med  2019    PubMed ID: 30735010   DOI: 10.1002/cam4.1949
12335  Mochizuki M, Nakamura M, Sibuya R, Okazaki T, Abe J, Nakagawa T, Takahashi S, Yamazaki T, Imai T, Takano A, Ito H, Yokose T, Miyagi Y, Daigo Y, Sato I, Satoh K, Sugamura K, Yamaguchi K, Tamai K.  CD271 is a negative prognostic factor and essential for cell proliferation in lung squamous cell carcinoma  Lab Invest  2019  99(9):1349-1362  PubMed ID: 31019292   DOI: 10.1038/s41374-019-0246-5
14238  Ozono K, Ohishi Y, Onishi H, Nakamura K, Motoshita J, Kato M, Nakanishi R, Nakamura M, Oda Y.  Brain-derived neurotrophic factor/tropomyosin-related kinase B signaling pathway contributes to the aggressive behavior of lung squamous cell carcinoma  Lab Invest  2017  97(11):1332-1342  PubMed ID: 28604655   DOI: 10.1038/labinvest.2017.45
9887  Ye X, Zhou Q, Matsumoto Y, Moriyama M, Kageyama S, Komatsu M, Satoh S, Tsuchida M, Saijo Y.  Inhibition of Glutaminolysis Inhibits Cell Growth via Down-regulating Mtorc1 Signaling in Lung Squamous Cell Carcinoma.  Anticancer Res.  2016  36:6021-6029  PubMed ID: 27793929   DOI: 10.21873/anticanres.11191
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144   DOI: 10.1073/pnas.1510077112
15909  Fang WT, Fan CC, Li SM, Jang TH, Lin HP, Shih NY, Chen CH, Wang TY, Huang SF, Lee AY, Liu YL, Tsai FY, Huang CT, Yang SJ, Yen LJ, Chuu CP, Chen CY, Hsiung CA, Chang JY, Wang LH, Chang IS, Jiang SS.  Downregulation of a putative tumor suppressor BMP4 by SOX2 promotes growth of lung squamous cell carcinoma  Int J Cancer  2014  135(4):809-19  PubMed ID: 24477565   DOI: 10.1002/ijc.28734
19029  Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest AR, Hayashizaki Y.  LRRN4 and UPK3B are markers of primary mesothelial cells  PLoS One  2011  6(10):e25391  PubMed ID: 21984916   DOI: 10.1371/journal.pone.0025391
16553  Hayashi S, Kumai T, Matsuda Y, Aoki N, Sato K, Kimura S, Kitada M, Tateno M, Celis E, Kobayashi H.  Six-transmembrane epithelial antigen of the prostate and enhancer of zeste homolog 2 as immunotherapeutic targets for lung cancer  J Transl Med  2011  9:191  PubMed ID: 22053850   DOI: 10.1186/1479-5876-9-191
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
18311  Mitsuoka K, Miyoshi S, Kato Y, Murakami Y, Utsumi R, Kubo Y, Noda A, Nakamura Y, Nishimura S, Tsuji A.  Cancer detection using a PET tracer, 11C-glycylsarcosine, targeted to H+/peptide transporter  J Nucl Med  2008  49(4):615-22  PubMed ID: 18344442   DOI: 10.2967/jnumed.107.048231
16648  Konishi J, Yamazaki K, Yokouchi H, Shinagawa N, Iwabuchi K, Nishimura M.  The characteristics of human NKT cells in lung cancer--CD1d independent cytotoxicity against lung cancer cells by NKT cells and decreased human NKT cell response in lung cancer patients  Hum Immunol  2004  65(11):1377-88  PubMed ID: 15556688   DOI: 10.1016/j.humimm.2004.09.003



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