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Cell No. : Cell Name
RCB1965 : EBC-1  update : 2020/09/24
CommentHuman lung squamous cell carcinoma cell line. TKG0481 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0481
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 69 years
Tissue lung
Case history Squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0481)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 5
User's Publication 6

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3289  Yamashita J, Tashiro K, Yoneda S, Kawahara K, Shirakusa T  Local increase in polymorphonuclear leukocyte elastase is associated with tumor invasiveness in non-small cell lung cancer.  Chest  1996  109:1328-34  PubMed ID: 8625686
3287  Takigawa N, Ohnoshi T, Ueoka H, Kiura K, Moritaka T, Tabata M, Segawa Y, Shibayama T, Gemba K, Matsumura T, et al.  [In vitro comparison of podophyllotoxin analogues; etoposide, teniposide and NK 611 using human lung cancer cell lines]  Gan To Kagaku Ryoho  1993  20:473-7  PubMed ID: 8383949
3288  Takigawa N, Ohnoshi T, Ueoka H, Horiguchi T, Kiura K, Tabata M, Segawa Y, Shibayama T, Genba K, Matsumura T, et al.  [Assessment of antitumor activity rhizoxin for human lung cancer cell lines: a potent new drug for drug-resistant lung cancer]  Gan To Kagaku Ryoho  1993  20:1221-6  PubMed ID: 8392827
3285  Kitamura M, Shirasawa T, Mitarai T, Muramatsu T, Maruyama N  A retinoid responsive cytokine gene, MK, is preferentially expressed in the proximal tubules of the kidney and human tumor cell lines.  Am J Pathol  1993  142:425-31  PubMed ID: 8434639
5059  Hiraki S, Miyai M, Seto T, Tamura T, Watanabe Y, Ozawa S, Ikeda H, Nakata Y, Ohnoshi T, Kimura I  Establishment of human continuous cell lines from squamous cell, adeno- and small cell carcinoma of the lung and the results of hetero-transplantation  Haigan  1982  22(1):53-8  

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User's Publication
12073  Haruna M, Hirata M, Iwahori K, Kanazawa T, Yamamoto Y, Goto K, Kawashima A, Morimoto-Okazawa A, Funaki S, Shintani Y, Kumanogoh A, Wada H.  Docetaxel Upregulates HMGB1 Levels in Non-small Cell Lung Cancer.  Biol. Pharm. Bull.  2020    PubMed ID: 32115500
4183  Ohara K, Ohkuri T, Kumai T, Nagato T, Nozaki Y, Ishibashi K, Kosaka A, Nagata M, Harabuchi S, Ohara M, Oikawa K, Aoki N, Harabuchi Y, Celis E, Kobayashi H.  Targeting phosphorylated p53 to elicit tumor-reactive T helper responses against head and neck squamous cell carcinoma.  Oncoimmunology  2018    PubMed ID: 30510853
4475  Iijima M, Gombodorj N, Tachibana Y, Tachibana K, Yokobori T, Honma K, Nakano T, Asao T, Kuwahara R, Aoyama K, Yasuda H, Kelly M, Kuwano H, Yamanouchi D.  Development of single nanometer-sized ultrafine oxygen bubbles to overcome the hypoxia-induced resistance to radiation therapy via the suppression of hypoxia-inducible factor‑1α.  Int. J. Oncol.  2018  52(3):679-686  PubMed ID: 29393397
9887  Ye X, Zhou Q, Matsumoto Y, Moriyama M, Kageyama S, Komatsu M, Satoh S, Tsuchida M, Saijo Y.  Inhibition of Glutaminolysis Inhibits Cell Growth via Down-regulating Mtorc1 Signaling in Lung Squamous Cell Carcinoma.  Anticancer Res.  2016  36:6021-6029  PubMed ID: 27793929
5843  Bladt F, Faden B, Friese-Hamim M, Knuehl C, Wilm C, Fittschen C, Grädler U, Meyring M, Dorsch D, Jaehrling F, Pehl U, Stieber F, Schadt O, Blaukat A.  EMD 1214063 and EMD 1204831 constitute a new class of potent and highly selective c-Met inhibitors.  Clin Cancer Res  2013  19(11):2941-51  PubMed ID: 23553846
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309

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