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Cell No. : Cell Name
RCB1955 : KMST-6  update : 2021/01/08
CommentHuman cell line derived from embryonic fibroblasts. Characteristics Embryonal fibroblast immortalized by 60-Co irradiation (400 Rad x 1 time and 200 rad x 12 times). Non-tumorigenic. TKG0482 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Namba, M.
Year of deposit 2004
Original cell TKG0482
Animal _human < Mammals
Genus Homo
Species sapiens
Age at sampling embryo
Tissue embryonal fibroblast
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0482)
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 25
User's Publication 3


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Reference
4633  Gao C, Miyazaki M, Kondo T, Tsuji T, Sakaguchi M, Namba M.  Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts.  Int J Oncol  2001  18(4):871-5  PubMed ID: 11251187
4646  Pu H, Sakaguchi M, Kondo T, Kondo A, Kawabata T, Namba M.  Effects of oxygen concentrations on human fibroblasts treated with Fe(3+)-NTA.  Int J Mol Med  2001  7(3):295-300  PubMed ID: 11179510
4645  Ohashi R, Miyazaki M, Fushimi K, Tsuji T, Inoue Y, Shimizu N, Namba M.  Enhanced activity of cyclin A-associated kinase in immortalized human fibroblasts.  Int J Cancer  1999  82(5):754-8  PubMed ID: 10417776
4644  Fushimi K, Torigoe K, Yamauchi H, Furusako S, Kurimoto M, Namba M.  Establishment of a human fibroblast cell line producing tumor necrosis factor alpha (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture.  In Vitro Cell Dev Biol Anim  1998  34(6):463-7  PubMed ID: 9661049
4621  Sugihara S, Mihara K, Marunouchi T, Inoue H, Namba M.  Telomere elongation observed in immortalized human fibroblasts by treatment with 60Co gamma rays or 4-nitroquinoline 1-oxide.  Hum Genet  1996  97(1):1-6  PubMed ID: 8557247
4643  Jahan I, Iijima M, Kondo T, Namba M.  Effects of okadaic acid on cell growth, anchorage-independent growth, and co-cultures of normal (KMS-6), immortalized (KMST-6), and neoplastically transformed (KMST-6T and KMST-6/RAS) human fibroblasts.  J Cancer Res Clin Oncol  1996  122(4):249-53  PubMed ID: 8601579
4629  Ogata T, Oshimura M, Namba M, Fujii M, Oishi M, Ayusawa D.  Genetic complementation of the immortal phenotype in group D cell lines by introduction of chromosome 7.  Jpn J Cancer Res  1995  86(1):35-40  PubMed ID: 7737907
4631  Kondo T, Mihara K, Inoue Y, Iijima M, Namba M.  Two-dimensional electrophoretic analysis of down-regulated proteins in human fibroblasts immortalized by treatment with either 4-nitroquinoline 1-oxide or 60Co gamma rays.  Electrophoresis  1995  16(6):1067-73  PubMed ID: 7498129
4642  Kawashima K1, Jahan I, Fushimi K, Mihara K, Namba M.  Human fibroblasts (KMST-6/RAS cell line) transformed with 60Co gamma-rays and c-Ha-ras oncogene produce a large amount of granulocyte colony-stimulating factor (G-CSF); production is enhanced by cAMP, theophylline, and butyrate.  Cell Struct Funct  1995  20(1):41-5  PubMed ID: 7540958
4640  Kawashima K, Mori M, Furusako S, Usuki H, Shimizu N, Namba M.  Granulocyte-colony stimulating factor (G-CSF) production of human fibroblasts (KMST-6/RAS line) transformed with 60Co gamma rays and c-Ha-ras oncogene.  In Vitro Cell Dev Biol Anim  1994  30A(4):199-201  PubMed ID: 7520808
4641  Kawashima K, Mori M, Furusako S, Usuki H, Namba M.  Human fibroblasts (KMST-6/RAS line) transformed with 60Co gamma-rays and c-Ha-ras oncogene constitutively produce a large amount of human granulocyte-colony stimulating factor (G-CSF).  Hum Cell  1994  7(2):88-94  PubMed ID: 7524638
4624  Ogata T, Ayusawa D, Namba M, Takahashi E, Oshimura M, Oishi M.  Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.  Mol Cell Biol  1993  13(10):6036-43  PubMed ID: 8105370
4628  Namba M, Iijima M, Kondo T, Jahan I, Mihara K.  Immortalization of normal human cells is a multistep process and a rate limiting step of neoplastic transformation of the cells.  Hum Cell  1993  6(4):253-9  PubMed ID: 8148305
4638  Endo A, Kano Y, Mihara K, Orita K, Namba M.  Alteration in the retinoblastoma gene associated with immortalization of human fibroblasts treated with 60Co gamma rays.  J Cancer Res Clin Oncol  1993  119(9):522-6  PubMed ID: 8100822
4639  Jahan I, Mihara K, Bai L, Namba M.  Neoplastic transformation and characterization of human fibroblasts by treatment with 60Co gamma rays and the human c-Ha-ras oncogene.  In Vitro Cell Dev Biol Anim  1993  29A(10):763-7  PubMed ID: 8118610
4627  Bai L, Naomoto Y, Miyazaki M, Orita K, Namba M.  Antiproliferative effects of suramin on human cancer cells in vitro and in vivo.  Acta Med Okayama  1992  46(6):457-63  PubMed ID: 1485540
4626  Bai LY, Miyazaki M, Namba M.  Effects of suramin on cell proliferation of various types of human malignant cells  Gan To Kagaku Ryoho  1991  18(3):387-91  PubMed ID: 1848419
4671  Hatamochi A, Ono M, Ueki H, Namba M.  Regulation of collagen gene expression by transformed human fibroblasts: decreased type I and type III collagen RNA transcription.  J Invest Dermatol  1991  96(4):473-7  PubMed ID: 1706744
4622  Namba M, Nishitani K, Fukushima F, Kimoto T.  Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.  Anticancer Res  1988  8(5A):947-58  PubMed ID: 3140712
4637  Namba M, Nishitani K, Fukushima F, Kimoto T, Yuasa Y.  Multi-step neoplastic transformation of normal human fibroblasts by Co-60 gamma rays and Ha-ras oncogenes.  Mutat Res  1988  199(2):415-23  PubMed ID: 3287150
4625  Namba M, Karai M, Kimoto T.  Comparison of major cytoskeletons among normal human fibroblasts, immortal human fibroblasts transformed by exposure to Co-60 gamma rays, and the latter cells made tumorigenic by treatment with Harvey murine sarcoma virus.  Exp Gerontol  1987  22(3):179-86  PubMed ID: 3305051
4636  Karai M, Namba M.  Interferon-beta can induce the production of plasminogen activator by cultured human cancer cells.  Experientia  1987  43(4):435-7  PubMed ID: 3569495
4635  Namba M, Nishitani K, Fukushima F, Kimoto T, Nose K.  Multistep process of neoplastic transformation of normal human fibroblasts by 60Co gamma rays and Harvey sarcoma viruses.  Int J Cancer  1986  37(3):419-23  PubMed ID: 3949423
1025  Namba M, Nishitani K, Hyodoh F, Fukushima F, Kimoto T  Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays.  Int J Cancer  1985  35:275-80  PubMed ID: 3972471
4634  Namba M.  Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with Co-60 gamma rays.  Carcinog Compr Surv  1985  9:217-31  PubMed ID: 4053076

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User's Publication
4488  Koyama S, Narita E, Shinohara N, Miyakoshi J.  Recovery kinetics of micronucleus formation by fractionated X-ray irradiation in various types of human cells.  J. Radiat. Res.  2018  59(5):547-554  PubMed ID: 29961812
5678  Koyama S, Narita E, Shinohara N, Miyakoshi J.  Effect of low-dose X-ray irradiation on micronucleus formation in human embryo, newborn and child cells.  Int J Radiat Biol  2016  92(12):790-795  PubMed ID: 27579525
1744  Gu.,Y., Miyazaki, M., Ohno, T. and Namba M.  Cytotoxicity Test of Ethanol Using Various Human Hepatoma and Other Cell Lines  Alternatives to Animal Testing and Experimentation(AATEX)  1993  2:1-5 



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