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Cell No. : Cell Name
RCB1954 : T98G  update : 2024/08/14
CommentHuman cell line derived from glioblastoma multiforme (Caucasian). Hyperpentaploid chromosome count. TKG0471 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0471
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 61 years
Tissue brain
Disease name glioblastoma multiforme tumor
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0471)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0556
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 57


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Reference
4618  Stein GH, Yanishevsky RM.  Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons.  Proc Natl Acad Sci U S A  1981  78(5):3025-9  PubMed ID: 6265932   DOI: 10.1073/pnas.78.5.3025
3267  Stein, G H  T98G: an anchorage-independent human tumor cell line that exhibits stationary phase G1 arrest in vitro.  J Cell Physiol  1979  99:43-54  PubMed ID: 222778   DOI: 10.1002/jcp.1040990107

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User's Publication
19808  Yamaguchi I, Katoh H.  Merlin/NF2 regulates SLC7A11/xCT expression and cell viability under glucose deprivation at high cell density in glioblastoma cells  J Biochem  2024  175(3):313-322  PubMed ID: 38102738   DOI: 10.1093/jb/mvad105
19869  Morimoto T, Nakazawa T, Maeoka R, Matsuda R, Nakamura M, Nishimura F, Yamada S, Nakagawa I, Park YS, Tsujimura T.  Bulk RNA sequencing reveals the comprehensive genetic characteristics of human cord blood-derived natural killer cells  Regen Ther  2024  25:367-376  PubMed ID: 38405180   DOI: 10.1016/j.reth.2024.02.002
21603  Morimoto T, Nakazawa T, Matsuda R, Maeoka R, Nishimura F, Nakamura M, Yamada S, Park YS, Tsujimura T, Nakagawa I.  Antitumor Effects of Intravenous Natural Killer Cell Infusion in an Orthotopic Glioblastoma Xenograft Murine Model and Gene Expression Profile Analysis.  Int J Mol Sci  2024  25(4)  PubMed ID: 38397112   DOI: 10.3390/ijms25042435
21638  Koh I, Hagiwara M.  Modular tissue-in-a-CUBE platform to model blood-brain barrier (BBB) and brain interaction.  Commun Biol  2024  7(1):177  PubMed ID: 38418614   DOI: 10.1038/s42003-024-05857-8
21674  Kinoshita S, Ishii M, Ando J, Kimura T, Yamaguchi T, Harada S, Takahashi F, Nakashima K, Nakazawa Y, Yamazaki S, Ohshima K, Takahashi K, Nakauchi H, Ando M.  Rejuvenated iPSC-derived GD2-directed CART Cells Harbor Robust Cytotoxicity Against Small Cell Lung Cancer.  Cancer Res Commun  2024  4(3):723-737  PubMed ID: 38380966   DOI: 10.1158/2767-9764.CRC-23-0259
19592  Maeoka R, Nakazawa T, Matsuda R, Morimoto T, Shida Y, Yamada S, Nishimura F, Nakamura M, Nakagawa I, Park YS, Tsujimura T, Nakase H.  Therapeutic Anti-KIR Antibody of 1-7F9 Attenuates the Antitumor Effects of Expanded and Activated Human Primary Natural Killer Cells on In Vitro Glioblastoma-like Cells and Orthotopic Tumors Derived Therefrom  Int J Mol Sci  2023  24(18):14183  PubMed ID: 37762486   DOI: 10.3390/ijms241814183
19770  Mitobe Y, Suzuki S, Nakagawa-Saito Y, Togashi K, Sugai A, Sonoda Y, Kitanaka C, Okada M.  Antagonizing MDM2 Overexpression Induced by MDM4 Inhibitor CEP-1347 Effectively Reactivates Wild-Type p53 in Malignant Brain Tumor Cells  Cancers (Basel)  2023  15(17):4326  PubMed ID: 37686602   DOI: 10.3390/cancers15174326
19946  Nakazawa T, Maeoka R, Morimoto T, Matsuda R, Nakamura M, Nishimura F, Yamada S, Nakagawa I, Park YS, Ito T, Nakase H, Tsujimura T.  An efficient feeder-free and chemically-defined expansion strategy for highly purified natural killer cells derived from human cord blood  Regen Ther  2023  24:32-42  PubMed ID: 37303464   DOI: 10.1016/j.reth.2023.05.006
19953  Nakazawa T, Morimoto T, Maeoka R, Matsuda R, Nakamura M, Nishimura F, Ouji N, Yamada S, Nakagawa I, Park YS, Ito T, Nakase H, Tsujimura T.  CIS deletion by CRISPR/Cas9 enhances human primary natural killer cell functions against allogeneic glioblastoma  J Exp Clin Cancer Res  2023  42(1):205  PubMed ID: 37563692   DOI: 10.1186/s13046-023-02770-6
21090  Li Y, Matsumoto Y, Chen L, Sugawara Y, Oe E, Fujisawa N, Ebara M, Sakurai H.  Smart Nanofiber Mesh with Locally Sustained Drug Release Enabled Synergistic Combination Therapy for Glioblastoma.  Nanomaterials (Basel)  2023  13(3)  PubMed ID: 36770373   DOI: 10.3390/nano13030414
21373  Suzuki MM, Iijima K, Ogami K, Shinjo K, Murofushi Y, Xie J, Wang X, Kitano Y, Mamiya A, Kibe Y, Nishimura T, Ohka F, Saito R, Sato S, Kobayashi J, Yao R, Miyata K, Kataoka K, Suzuki HI, Kondo Y.  TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.  Nat Commun  2023  14(1):4521  PubMed ID: 37607907   DOI: 10.1038/s41467-023-40243-8
21380  Tagawa Y, Sakagami H, Tanuma SI, Amano S, Uota S, Bandow K, Tomomura M, Uesawa Y, Takao K, Sugita Y, Yamamoto N, Sakashita H, Nakakaji R, Koizumi T, Mitsudo K, Tohnai I.  Potentiation of Anticancer Activity of G2/M Blockers by Mild Hyperthermia.  Anticancer Res  2023  43(8):3429-3439  PubMed ID: 37500171   DOI: 10.21873/anticanres.16518
17698  Yuji Doi, Daigo Wakana, Satoshi Kitaoka, Hisashi Takeda, Eiji Tanaka, Tomoo Hosoe  Secalonic Acid and Benzoic Acid Analogues Exhibiting Cyto toxicity against Cancer Cells Isolated from <i>Claviceps yanagawaensis</i>  Advances in Microbiology  2022  12:649-670    DOI: 10.4236/aim.2022.1212045
20564  Hayashima K, Katoh H.  Expression of gamma-glutamyltransferase 1 in glioblastoma cells confers resistance to cystine deprivation-induced ferroptosis.  J Biol Chem  2022  101703  PubMed ID: 35148992   DOI: 10.1016/j.jbc.2022.101703
20607  Shinohara Y, Ando H, Maekawa M, Arai M, Horibata Y, Satou M, Jojima T, Usui I, Aso Y, Sugimoto H.  Transcription of cytochrome P450 46A1 in NIH3T3 cells is negatively regulated by FBS.  Biochim Biophys Acta Mol Cell Biol Lipids  2022  1867(6):159136  PubMed ID: 35306146   DOI: 10.1016/j.bbalip.2022.159136
20874  Mitobe Y, Nakagawa-Saito Y, Togashi K, Suzuki S, Sugai A, Matsuda KI, Sonoda Y, Kitanaka C, Okada M.  CEP-1347 Targets MDM4 Protein Expression to Activate p53 and Inhibit the Growth of Glioma Cells.  Anticancer Res  2022  42(10):4727-4733  PubMed ID: 36192008   DOI: 10.21873/anticanres.15977
20884  Shindo A, Kusano M, Sakagami H, Amano S, Inomata M, Abe M, Okazawa M, Ooka T.  Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells.  In Vivo  2022  36(5):2116-2125  PubMed ID: 36099100   DOI: 10.21873/invivo.12937
13413  Hayashima K, Kimura I, Katoh H.  Role of ferritinophagy in cystine deprivation-induced cell death in glioblastoma cells.  Biochem Biophys Res Commun  2021    PubMed ID: 33421769   DOI: 10.1016/j.bbrc.2020.12.075
12121  Yamaguchi I, Yoshimura SH, Katoh H.  High cell density increases glioblastoma cell viability under glucose deprivation via degradation of the cystine/glutamate transporter xCT (SLC7A11).  J. Biol. Chem.  2020    PubMed ID: 32265299   DOI: 10.1074/jbc.RA119.012213
12999  Kinashi Y, Ikawa T, Takahashi S.  The combined effect of neutron irradiation and temozolomide on glioblastoma cell lines with different MGMT and P53 status.  Appl Radiat Isot  2020    PubMed ID: 32561044   DOI: 10.1016/j.apradiso.2020.109204
11419  Feng S, Cai X, Li Y, Jian X, Zhang L, Li B.  Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells.  J. Exp. Clin. Cancer Res.  2019    PubMed ID: 30728039   DOI: 10.1186/s13046-019-1070-x
4672  Ohka F, Shinjo K, Deguchi S, Matsui Y, Okuno Y, Katsushima K, Suzuki M, Kato A, Ogiso N, Yamamichi A, Aoki K, Suzuki H, Sato S, Arul Rayan N, Prabhakar S, Göke J, Shimamura T, Maruyama R, Takahashi S, Suzumura A, Kimura H, Wakabayashi T, Zong H, Natsume A, Kondo Y.  Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-wild-type Diffuse Astrocytic Gliomas.  Cancer Res  2019  79(19):4814-4827  PubMed ID: 31431463   DOI: 10.1158/0008-5472.CAN-19-1272
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217   DOI: 10.1016/j.bbrep.2019.100632
6953  Imaeda A, Tomoike F, Hayakawa M, Nakamoto K, Kimura Y, Abe N, Abe H.  N6-methyl adenosine in siRNA evades immune response without reducing RNAi activity.  Nucleosides Nucleotides Nucleic Acids  2019  38(12):972-979  PubMed ID: 31298608   DOI: 10.1080/15257770.2019.1641205
12144  Ranjit M, Hirano M, Aoki K, Okuno Y, Ohka F, Yamamichi A, Kato A, Maeda S, Motomura K, Matsuo K, Enomoto A, Ino Y, Todo T, Takahashi M, Wakabayashi T, Kato T, Natsume A.  Aberrant Active cis-Regulatory Elements Associated with Downregulation of RET Finger Protein Overcome Chemoresistance in Glioblastoma  Cell Rep  2019  2274-2281.e5  PubMed ID: 30811978   DOI: 10.1016/j.celrep.2019.01.109
12593  Qiu W, Song S, Chen W, Zhang J, Yang H, Chen Y.  Hypoxia-induced EPHB2 promotes invasive potential of glioblastoma  Int J Clin Exp Pathol  2019  12(2):539-548  PubMed ID: 31933858  
14811  Fan L, Chen Z, Wu X, Cai X, Feng S, Lu J, Wang H, Liu N.  Ubiquitin-Specific Protease 3 Promotes Glioblastoma Cell Invasion and Epithelial-Mesenchymal Transition via Stabilizing Snail  Mol Cancer Res  2019  17(10):1975-1984  PubMed ID: 31266817   DOI: 10.1158/1541-7786.MCR-19-0197
4353  Peng F, Wang X, Shu M, Yang M, Wang L, Ouyang Z, Shen C, Hou X, Zhao B, Wang X, Zhang LW, Liu Y, Zhao S.  Raddeanin a Suppresses Glioblastoma Growth by Inducing ROS Generation and Subsequent JNK Activation to Promote Cell Apoptosis.  Cell. Physiol. Biochem.  2018  47(3):1108-1121  PubMed ID: 29843152   DOI: 10.1159/000490187
4375  Fukushima T, Kawaguchi M, Yamamoto K, Yamashita F, Izumi A, Kaieda T, Takezaki Y, Itoh H, Takeshima H, Kataoka H.  Aberrant methylation and silencing of the SPINT2 gene in high-grade gliomas.  Cancer Sci.  2018  109(9):2970-2979  PubMed ID: 29987920   DOI: 10.1111/cas.13732
4503  Ishiguro Y, Kobayashi M, Ideno M, Narumi K, Furugen A, Iseki K.  Valproate sensitizes human glioblastoma cells to 3-bromopyruvate-induced cytotoxicity.  Int J Pharm  2018  551(1-2):97-102  PubMed ID: 30138705   DOI: 10.1016/j.ijpharm.2018.08.039
14903  Zhao B, Shen C, Zheng Z, Wang X, Zhao W, Chen X, Peng F, Xue L, Shu M, Hou X, Wang K, Zhong C, Sun J, Wan J, Zhao S.  Peiminine Inhibits Glioblastoma in Vitro and in Vivo Through Cell Cycle Arrest and Autophagic Flux Blocking  Cell Physiol Biochem  2018  51(4):1566-1583  PubMed ID: 30497066   DOI: 10.1159/000495646
10052  Shinoda Y, Takahashi T, Akimoto J, Ichikawa M, Yamazaki H, Narumi A, Yano S, Fujiwara Y.  Comparative photodynamic therapy cytotoxicity of mannose-conjugated chlorin and talaporfin sodium in cultured human and rat cells.  J Toxicol Sci  2017  42:111-119  PubMed ID: 28070104   DOI: 10.2131/jts.42.111
10523  Asano M, Tanaka S, Sakaguchi M, Matsumura H, Yamaguchi T, Fujita Y, Tabuse K.  Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis.  Sci Rep  2017  7:11406  PubMed ID: 28900243   DOI: 10.1038/s41598-017-11784-y
10559  Goji T, Takahara K, Negishi M, Katoh H.  Cystine uptake through the cystine/glutamate antiporter xCT triggers glioblastoma cell death under glucose deprivation.  J. Biol. Chem.  2017  292:19721-19732  PubMed ID: 29038291   DOI: 10.1074/jbc.M117.814392
5680  Ginting TE, Suryatenggara J, Christian S, Mathew G.  Proinflammatory response induced by Newcastle disease virus in tumor and normal cells.  Oncolytic Virother  2017  6:21-30  PubMed ID: 28293547   DOI: 10.2147/OV.S123292
14208  Miyata K, Takemoto A, Okumura S, Nishio M, Fujita N.  Podoplanin enhances lung cancer cell growth in vivo by inducing platelet aggregation  Sci Rep  2017  7(1):4059  PubMed ID: 28642617   DOI: 10.1038/s41598-017-04324-1
18189  Peng C, Chen Z, Wang S, Wang HW, Qiu W, Zhao L, Xu R, Luo H, Chen Y, Chen D, You Y, Liu N, Wang H.  The Error-Prone DNA Polymerase κ Promotes Temozolomide Resistance in Glioblastoma through Rad17-Dependent Activation of ATR-Chk1 Signaling  Cancer Res  2016  76(8):2340-53  PubMed ID: 26960975   DOI: 10.1158/0008-5472.CAN-15-1884
7299  Miki Y, Akimoto J, Moritake K, Hironaka C, Fujiwara Y.  Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells.  Lasers Med Sci  2015  30:1739-45  PubMed ID: 26109138   DOI: 10.1007/s10103-015-1783-9
7547  Miki Y., Akimoto J., Sato A., Fujiwara Y.  Evaluation of laser irradiance on photodynamic therapy using talaporfin sodium-induced glioblastoma T98G cell death.  Fundamental Toxicological Sciences  2015  2:111-116    DOI: 10.2131/fts.2.111
7557  Seki K, Kinashi Y, Takahashi S.  Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.  Anticancer Res.  2015  35:169-74  PubMed ID: 25550548  
15122  Liu H, Li C, Shen C, Yin F, Wang K, Liu Y, Zheng B, Zhang W, Hou X, Chen X, Wu J, Wang X, Zhong C, Zhang J, Shi H, Ai J, Zhao S.  MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3  J Neurooncol  2015  122(3):431-9  PubMed ID: 25720694   DOI: 10.1007/s11060-015-1736-y
15156  Hirohara S, Oka C, Totani M, Obata M, Yuasa J, Ito H, Tamura M, Matsui H, Kakiuchi K, Kawai T, Kawaichi M, Tanihara M.  Synthesis, Photophysical Properties, and Biological Evaluation of trans-Bisthioglycosylated Tetrakis(fluorophenyl)chlorin for Photodynamic Therapy  J Med Chem  2015  58(21):8658-70  PubMed ID: 26457363   DOI: 10.1021/acs.jmedchem.5b01262
15102  Sasaki S, Futagi Y, Kobayashi M, Ogura J, Iseki K.  Functional characterization of 5-oxoproline transport via SLC16A1/MCT1  J Biol Chem  2015  290(4):2303-11  PubMed ID: 25371203   DOI: 10.1074/jbc.M114.581892
17085  Kinashi Y, Okumura K, Kubota Y, Kitajima E, Okayasu R, Ono K, Takahashi S.  Dose-rate effect was observed in T98G glioma cells following BNCT  Appl Radiat Isot  2014  88:81-5  PubMed ID: 24360864   DOI: 10.1016/j.apradiso.2013.11.117
19076  Zhao SG, Chen XF, Wang LG, Yang G, Han DY, Teng L, Yang MC, Wang DY, Shi C, Liu YH, Zheng BJ, Shi CB, Gao X, Rainov NG.  Increased expression of ABCB6 enhances protoporphyrin IX accumulation and photodynamic effect in human glioma  Ann Surg Oncol  2013  20(13):4379-88  PubMed ID: 22688660   DOI: 10.1245/s10434-011-2201-6
13710  Fukushima T, Kawaguchi M, Yorita K, Tanaka H, Takeshima H, Umezawa K, Kataoka H.  Antitumor effect of dehydroxymethylepoxyquinomicin, a small molecule inhibitor of nuclear factor-κB, on glioblastoma  Neuro Oncol  2012  14(1):19-28  PubMed ID: 21968049   DOI: 10.1093/neuonc/nor168
15728  Matsuda K, Sato A, Okada M, Shibuya K, Seino S, Suzuki K, Watanabe E, Narita Y, Shibui S, Kayama T, Kitanaka C.  Targeting JNK for therapeutic depletion of stem-like glioblastoma cells  Sci Rep  2012  2:516  PubMed ID: 22816039   DOI: 10.1038/srep00516
6312  Ohsaka, Y. & Nishino, H.  Normal Japanese individuals harbor polymorphisms in the p14 ARF /INK4 locus promoters and/or other gene introns. — Variation in nucleotide sequences in each individual  Genes Genom  2011  33: 621    DOI: 10.1007/s13258-011-0085-2
7797  Ohsaka Y, Nishino H.  Polymorphisms in promoter sequences of MDM2, p53, and p16 genes in normal Japanese individuals.  Genet. Mol. Biol.  2010  33:615-26  PubMed ID: 21637567   DOI: 10.1590/s1415-47572010000400004
7905  Tomiyama A, Tachibana K, Suzuki K, Seino S, Sunayama J, Matsuda KI, Sato A, Matsumoto Y, Nomiya T, Nemoto K, Yamashita H, Kayama T, Ando K, Kitanaka C.  MEK-ERK-dependent multiple caspase activation by mitochondrial proapoptotic Bcl-2 family proteins is essential for heavy ion irradiation-induced glioma cell death.  Cell Death Dis  2010  1:e60  PubMed ID: 21364665   DOI: 10.1038/cddis.2010.37
8109  Ohsaka Y, Yogosawa S, Nakanishi R, Sakai T, Nishino H.  Polymorphisms in promoter sequences of the p15 ( INK4B ) and PTEN genes of normal Japanese individuals.  Biochem. Genet.  2010  48:970-86  PubMed ID: 20862607   DOI: 10.1007/s10528-010-9379-3
16551  Song X, Chen Z, Wu C, Zhao S.  Abrogating HSP response augments cell death induced by As2O3 in glioma cell lines  Can J Neurol Sci  2010  37(4):504-11  PubMed ID: 20724260   DOI: 10.1017/s0317167100010544
18304  Zhao S, Zhang J, Zhang X, Dong X, Sun X.  Arsenic trioxide induces different gene expression profiles of genes related to growth and apoptosis in glioma cells dependent on the p53 status  Mol Biol Rep  2008  35(3):421-9  PubMed ID: 17530438   DOI: 10.1007/s11033-007-9102-6
3698  Fukushima T, Tezuka T, Shimomura T, Nakano S, Kataoka H.  Silencing of insulin-like growth factor-binding protein-2 in human glioblastoma cells reduces both invasiveness and expression of progression-associated gene CD24.  J Biol Chem  2007  282(25):18634-44  PubMed ID: 17475624   DOI: 10.1074/jbc.M609567200
18714  Genkai N, Homma J, Sano M, Tanaka R, Yamanaka R.  Increased expression of pituitary tumor-transforming gene (PTTG)-1 is correlated with poor prognosis in glioma patients  Oncol Rep  2006  15(6):1569-74  PubMed ID: 16685397  
14417  Sano M, Genkai N, Yajima N, Tsuchiya N, Homma J, Tanaka R, Miki T, Yamanaka R.  Expression level of ECT2 proto-oncogene correlates with prognosis in glioma patients  Oncol Rep  2006  16(5):1093-8  PubMed ID: 17016598  
14418  Homma J, Yamanaka R, Yajima N, Tsuchiya N, Genkai N, Sano M, Tanaka R.  Increased expression of CCAAT/enhancer binding protein beta correlates with prognosis in glioma patients  Oncol Rep  2006  15(3):595-601  PubMed ID: 16465418  



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