Cell No. : Cell Name RCB1954 : T98G  update : 2023/02/09
Comment			Human cell line derived from glioblastoma multiforme (Caucasian). Hyperpentaploid chromosome count. TKG0471 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Year of deposit	2004
		Original cell	TKG0471
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	61 years
		Tissue	brain
		Disease name	glioblastoma multiforme tumor
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0471）
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_0556
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	41

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Reference

4618  Stein GH, Yanishevsky RM.  Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons.  Proc Natl Acad Sci U S A  1981  78(5):3025-9  PubMed ID: 6265932   DOI: 10.1073/pnas.78.5.3025

3267  Stein, G H  T98G: an anchorage-independent human tumor cell line that exhibits stationary phase G1 arrest in vitro.  J Cell Physiol  1979  99:43-54  PubMed ID: 222778   DOI: 10.1002/jcp.1040990107

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User's Publication

21090  Li Y, Matsumoto Y, Chen L, Sugawara Y, Oe E, Fujisawa N, Ebara M, Sakurai H.  Smart Nanofiber Mesh with Locally Sustained Drug Release Enabled Synergistic Combination Therapy for Glioblastoma.  Nanomaterials (Basel)  2023  13(3)  PubMed ID: 36770373   DOI: 10.3390/nano13030414

20564  Hayashima K, Katoh H.  Expression of gamma-glutamyltransferase 1 in glioblastoma cells confers resistance to cystine deprivation-induced ferroptosis.  J Biol Chem  2022  101703  PubMed ID: 35148992   DOI: 10.1016/j.jbc.2022.101703

20607  Shinohara Y, Ando H, Maekawa M, Arai M, Horibata Y, Satou M, Jojima T, Usui I, Aso Y, Sugimoto H.  Transcription of cytochrome P450 46A1 in NIH3T3 cells is negatively regulated by FBS.  Biochim Biophys Acta Mol Cell Biol Lipids  2022  1867(6):159136  PubMed ID: 35306146   DOI: 10.1016/j.bbalip.2022.159136

20874  Mitobe Y, Nakagawa-Saito Y, Togashi K, Suzuki S, Sugai A, Matsuda KI, Sonoda Y, Kitanaka C, Okada M.  CEP-1347 Targets MDM4 Protein Expression to Activate p53 and Inhibit the Growth of Glioma Cells.  Anticancer Res  2022  42(10):4727-4733  PubMed ID: 36192008   DOI: 10.21873/anticanres.15977

20884  Shindo A, Kusano M, Sakagami H, Amano S, Inomata M, Abe M, Okazawa M, Ooka T.  Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells.  In Vivo  2022  36(5):2116-2125  PubMed ID: 36099100   DOI: 10.21873/invivo.12937

13413  Hayashima K, Kimura I, Katoh H.  Role of ferritinophagy in cystine deprivation-induced cell death in glioblastoma cells.  Biochem Biophys Res Commun  2021    PubMed ID: 33421769   DOI: 10.1016/j.bbrc.2020.12.075

12121  Yamaguchi I, Yoshimura SH, Katoh H.  High cell density increases glioblastoma cell viability under glucose deprivation via degradation of the cystine/glutamate transporter xCT (SLC7A11).  J. Biol. Chem.  2020    PubMed ID: 32265299   DOI: 10.1074/jbc.RA119.012213

12999  Kinashi Y, Ikawa T, Takahashi S.  The combined effect of neutron irradiation and temozolomide on glioblastoma cell lines with different MGMT and P53 status.  Appl Radiat Isot  2020    PubMed ID: 32561044   DOI: 10.1016/j.apradiso.2020.109204

11419  Feng S, Cai X, Li Y, Jian X, Zhang L, Li B.  Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells.  J. Exp. Clin. Cancer Res.  2019    PubMed ID: 30728039   DOI: 10.1186/s13046-019-1070-x

4672  Ohka F, Shinjo K, Deguchi S, Matsui Y, Okuno Y, Katsushima K, Suzuki M, Kato A, Ogiso N, Yamamichi A, Aoki K, Suzuki H, Sato S, Arul Rayan N, Prabhakar S, Göke J, Shimamura T, Maruyama R, Takahashi S, Suzumura A, Kimura H, Wakabayashi T, Zong H, Natsume A, Kondo Y.  Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-wild-type Diffuse Astrocytic Gliomas.  Cancer Res  2019  79(19):4814-4827  PubMed ID: 31431463   DOI: 10.1158/0008-5472.CAN-19-1272

11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217   DOI: 10.1016/j.bbrep.2019.100632

6953  Imaeda A, Tomoike F, Hayakawa M, Nakamoto K, Kimura Y, Abe N, Abe H.  N6-methyl adenosine in siRNA evades immune response without reducing RNAi activity.  Nucleosides Nucleotides Nucleic Acids  2019  38(12):972-979  PubMed ID: 31298608   DOI: 10.1080/15257770.2019.1641205

12144  Ranjit M, Hirano M, Aoki K, Okuno Y, Ohka F, Yamamichi A, Kato A, Maeda S, Motomura K, Matsuo K, Enomoto A, Ino Y, Todo T, Takahashi M, Wakabayashi T, Kato T, Natsume A.  Aberrant Active cis-Regulatory Elements Associated with Downregulation of RET Finger Protein Overcome Chemoresistance in Glioblastoma  Cell Rep  2019  2274-2281.e5  PubMed ID: 30811978   DOI: 10.1016/j.celrep.2019.01.109

12593  Qiu W, Song S, Chen W, Zhang J, Yang H, Chen Y.  Hypoxia-induced EPHB2 promotes invasive potential of glioblastoma  Int J Clin Exp Pathol  2019  12(2):539-548  PubMed ID: 31933858

14811  Fan L, Chen Z, Wu X, Cai X, Feng S, Lu J, Wang H, Liu N.  Ubiquitin-Specific Protease 3 Promotes Glioblastoma Cell Invasion and Epithelial-Mesenchymal Transition via Stabilizing Snail  Mol Cancer Res  2019  17(10):1975-1984  PubMed ID: 31266817   DOI: 10.1158/1541-7786.MCR-19-0197

4353  Peng F, Wang X, Shu M, Yang M, Wang L, Ouyang Z, Shen C, Hou X, Zhao B, Wang X, Zhang LW, Liu Y, Zhao S.  Raddeanin a Suppresses Glioblastoma Growth by Inducing ROS Generation and Subsequent JNK Activation to Promote Cell Apoptosis.  Cell. Physiol. Biochem.  2018  47(3):1108-1121  PubMed ID: 29843152   DOI: 10.1159/000490187

4375  Fukushima T, Kawaguchi M, Yamamoto K, Yamashita F, Izumi A, Kaieda T, Takezaki Y, Itoh H, Takeshima H, Kataoka H.  Aberrant methylation and silencing of the SPINT2 gene in high-grade gliomas.  Cancer Sci.  2018  109(9):2970-2979  PubMed ID: 29987920   DOI: 10.1111/cas.13732

4503  Ishiguro Y, Kobayashi M, Ideno M, Narumi K, Furugen A, Iseki K.  Valproate sensitizes human glioblastoma cells to 3-bromopyruvate-induced cytotoxicity.  Int J Pharm  2018  551(1-2):97-102  PubMed ID: 30138705   DOI: 10.1016/j.ijpharm.2018.08.039

14903  Zhao B, Shen C, Zheng Z, Wang X, Zhao W, Chen X, Peng F, Xue L, Shu M, Hou X, Wang K, Zhong C, Sun J, Wan J, Zhao S.  Peiminine Inhibits Glioblastoma in Vitro and in Vivo Through Cell Cycle Arrest and Autophagic Flux Blocking  Cell Physiol Biochem  2018  51(4):1566-1583  PubMed ID: 30497066   DOI: 10.1159/000495646

10052  Shinoda Y, Takahashi T, Akimoto J, Ichikawa M, Yamazaki H, Narumi A, Yano S, Fujiwara Y.  Comparative photodynamic therapy cytotoxicity of mannose-conjugated chlorin and talaporfin sodium in cultured human and rat cells.  J Toxicol Sci  2017  42:111-119  PubMed ID: 28070104   DOI: 10.2131/jts.42.111

10523  Asano M, Tanaka S, Sakaguchi M, Matsumura H, Yamaguchi T, Fujita Y, Tabuse K.  Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis.  Sci Rep  2017  7:11406  PubMed ID: 28900243   DOI: 10.1038/s41598-017-11784-y

10559  Goji T, Takahara K, Negishi M, Katoh H.  Cystine uptake through the cystine/glutamate antiporter xCT triggers glioblastoma cell death under glucose deprivation.  J. Biol. Chem.  2017  292:19721-19732  PubMed ID: 29038291   DOI: 10.1074/jbc.M117.814392

5680  Ginting TE, Suryatenggara J, Christian S, Mathew G.  Proinflammatory response induced by Newcastle disease virus in tumor and normal cells.  Oncolytic Virother  2017  6:21-30  PubMed ID: 28293547   DOI: 10.2147/OV.S123292

14208  Miyata K, Takemoto A, Okumura S, Nishio M, Fujita N.  Podoplanin enhances lung cancer cell growth in vivo by inducing platelet aggregation  Sci Rep  2017  7(1):4059  PubMed ID: 28642617   DOI: 10.1038/s41598-017-04324-1

7299  Miki Y, Akimoto J, Moritake K, Hironaka C, Fujiwara Y.  Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells.  Lasers Med Sci  2015  30:1739-45  PubMed ID: 26109138   DOI: 10.1007/s10103-015-1783-9

7547  Miki Y., Akimoto J., Sato A., Fujiwara Y.  Evaluation of laser irradiance on photodynamic therapy using talaporfin sodium-induced glioblastoma T98G cell death.  Fundamental Toxicological Sciences  2015  2:111-116    DOI: 10.2131/fts.2.111

7557  Seki K, Kinashi Y, Takahashi S.  Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.  Anticancer Res.  2015  35:169-74  PubMed ID: 25550548

15122  Liu H, Li C, Shen C, Yin F, Wang K, Liu Y, Zheng B, Zhang W, Hou X, Chen X, Wu J, Wang X, Zhong C, Zhang J, Shi H, Ai J, Zhao S.  MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3  J Neurooncol  2015  122(3):431-9  PubMed ID: 25720694   DOI: 10.1007/s11060-015-1736-y

15156  Hirohara S, Oka C, Totani M, Obata M, Yuasa J, Ito H, Tamura M, Matsui H, Kakiuchi K, Kawai T, Kawaichi M, Tanihara M.  Synthesis, Photophysical Properties, and Biological Evaluation of trans-Bisthioglycosylated Tetrakis(fluorophenyl)chlorin for Photodynamic Therapy  J Med Chem  2015  58(21):8658-70  PubMed ID: 26457363   DOI: 10.1021/acs.jmedchem.5b01262

15102  Sasaki S, Futagi Y, Kobayashi M, Ogura J, Iseki K.  Functional characterization of 5-oxoproline transport via SLC16A1/MCT1  J Biol Chem  2015  290(4):2303-11  PubMed ID: 25371203   DOI: 10.1074/jbc.M114.581892

17085  Kinashi Y, Okumura K, Kubota Y, Kitajima E, Okayasu R, Ono K, Takahashi S.  Dose-rate effect was observed in T98G glioma cells following BNCT  Appl Radiat Isot  2014  88:81-5  PubMed ID: 24360864   DOI: 10.1016/j.apradiso.2013.11.117

13710  Fukushima T, Kawaguchi M, Yorita K, Tanaka H, Takeshima H, Umezawa K, Kataoka H.  Antitumor effect of dehydroxymethylepoxyquinomicin, a small molecule inhibitor of nuclear factor-κB, on glioblastoma  Neuro Oncol  2012  14(1):19-28  PubMed ID: 21968049   DOI: 10.1093/neuonc/nor168

15728  Matsuda K, Sato A, Okada M, Shibuya K, Seino S, Suzuki K, Watanabe E, Narita Y, Shibui S, Kayama T, Kitanaka C.  Targeting JNK for therapeutic depletion of stem-like glioblastoma cells  Sci Rep  2012  2:516  PubMed ID: 22816039   DOI: 10.1038/srep00516

6312  Ohsaka, Y. & Nishino, H.  Normal Japanese individuals harbor polymorphisms in the p14 ARF /INK4 locus promoters and/or other gene introns. — Variation in nucleotide sequences in each individual  Genes Genom  2011  33: 621    DOI: 10.1007/s13258-011-0085-2

7797  Ohsaka Y, Nishino H.  Polymorphisms in promoter sequences of MDM2, p53, and p16 genes in normal Japanese individuals.  Genet. Mol. Biol.  2010  33:615-26  PubMed ID: 21637567   DOI: 10.1590/s1415-47572010000400004

7905  Tomiyama A, Tachibana K, Suzuki K, Seino S, Sunayama J, Matsuda KI, Sato A, Matsumoto Y, Nomiya T, Nemoto K, Yamashita H, Kayama T, Ando K, Kitanaka C.  MEK-ERK-dependent multiple caspase activation by mitochondrial proapoptotic Bcl-2 family proteins is essential for heavy ion irradiation-induced glioma cell death.  Cell Death Dis  2010  1:e60  PubMed ID: 21364665   DOI: 10.1038/cddis.2010.37

8109  Ohsaka Y, Yogosawa S, Nakanishi R, Sakai T, Nishino H.  Polymorphisms in promoter sequences of the p15 ( INK4B ) and PTEN genes of normal Japanese individuals.  Biochem. Genet.  2010  48:970-86  PubMed ID: 20862607   DOI: 10.1007/s10528-010-9379-3

16551  Song X, Chen Z, Wu C, Zhao S.  Abrogating HSP response augments cell death induced by As2O3 in glioma cell lines  Can J Neurol Sci  2010  37(4):504-11  PubMed ID: 20724260   DOI: 10.1017/s0317167100010544

3698  Fukushima T, Tezuka T, Shimomura T, Nakano S, Kataoka H.  Silencing of insulin-like growth factor-binding protein-2 in human glioblastoma cells reduces both invasiveness and expression of progression-associated gene CD24.  J Biol Chem  2007  282(25):18634-44  PubMed ID: 17475624   DOI: 10.1074/jbc.M609567200

14417  Sano M, Genkai N, Yajima N, Tsuchiya N, Homma J, Tanaka R, Miki T, Yamanaka R.  Expression level of ECT2 proto-oncogene correlates with prognosis in glioma patients  Oncol Rep  2006  16(5):1093-8  PubMed ID: 17016598

14418  Homma J, Yamanaka R, Yajima N, Tsuchiya N, Genkai N, Sano M, Tanaka R.  Increased expression of CCAAT/enhancer binding protein beta correlates with prognosis in glioma patients  Oncol Rep  2006  15(3):595-601  PubMed ID: 16465418