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Cell No. : Cell Name
RCB1953 : NB-1  update : 2023/01/05
CommentHuman cell line derived from neuroblastoma. Dibuty-cAMP (1 mM) induces differentiation. TKG0486 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Miyake, S.
Year of deposit 2004
Original cell TKG0486
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 2 years
Tissue sympatho adrenal cell
Disease name neuroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University (TKG0471)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1440
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium (MEM : RPMI1640 = 1 : 1) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 8
User's Publication 9


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Reference
3264  Andrich MP, Shalaby-Rana E, Movassaghi N, Majd M.  The role of 131 iodine-metaiodobenzylguanidine scanning in the correlative imaging of patients with neuroblastoma.  Pediatrics  1996  97:246-250  PubMed ID: 8584386  
3265  Mastrangelo R, Tornesello A, Riccardi R, Lasorella A, Mastrangelo S, Mancini A, Rufini V, Troncone L  A new approach in the treatment of stage IV neuroblastoma using a combination of [131I]meta-iodobenzylguanidine (MIBG) and cisplatin.  Eur J Cancer  1995  31A:606-11  PubMed ID: 7576979   DOI: 10.1016/0959-8049(95)00048-n
3266  Kunimoto M  Possible involvement of the 440 kDa isoform of ankyrinB in neuritogenesis in human neuroblastoma NB-1 cells.  FEBS Lett  1995  357:217-20  PubMed ID: 7805894   DOI: 10.1016/0014-5793(94)01363-6
3263  Kunimoto M, Suzuki T  Selective down-regulation of 440 kDa ankyrinB associated with neurite retraction.  Neuroreport  1995  6:2545-8  PubMed ID: 8741759   DOI: 10.1097/00001756-199512150-00023
3261  Ohnuma N, Kasuga T, Nojiri I, Furuse T.  Radiosensitivity of human neuroblastoma cell line (NB-1)  Gann  1977  68:711-712  PubMed ID: 590688  
1648  Ishikawa S  Differentiation of human neuroblastoma cells in vitro--morphological changes induced by dibutyrl cyclic AMP.  Acta Pathol Jpn  1977  27:697-711  PubMed ID: 201151   DOI: 10.1111/j.1440-1827.1977.tb00186.x
3259  Miyake S, Shimo Y, Kitamura T.  Morphological differentiation in vitro of human continuous and functional neuroblastoma cell line, NB-I under treatment of (But)2cAMP (author's transl).  Noshinkei Geka  1975  3:407-414  PubMed ID: 174024  
3260  Imashuku S, Inui A, Nakamura T, Tanaka J, Miyake S.  Catecholamine metabolism in tissue culture cells of a neuroblastoma.  J Clin Endocrinol Metab  1973  36:931-936  PubMed ID: 4144593   DOI: 10.1210/jcem-36-5-931

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User's Publication
17079  Müller M, Rösch L, Najafi S, Gatzweiler C, Ridinger J, Gerloff XF, Jones DTW, Baßler J, Kreth S, Stainczyk S, Frese K, Meder B, Westermann F, Milde T, Peterziel H, Witt O, Oehme I.  Combining APR-246 and HDAC-Inhibitors: A Novel Targeted Treatment Option for Neuroblastoma  Cancers (Basel)  2021  13(17):4476  PubMed ID: 34503286   DOI: 10.3390/cancers13174476
10780  Shen J, Najafi S, Stäble S, Fabian J, Koeneke E, Kolbinger FR, Wrobel JK, Meder B, Distel M, Heimburg T, Sippl W, Jung M, Peterziel H, Kranz D, Boutros M, Westermann F, Witt O, Oehme I.  A kinome-wide RNAi screen identifies ALK as a target to sensitize neuroblastoma cells for HDAC8-inhibitor treatment.  Cell Death Differ.  2018  25(12):2053-2070  PubMed ID: 29515255   DOI: 10.1038/s41418-018-0080-0
9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427   DOI: 10.3892/ijo.2018.4251
10457  Watanabe Y, Ishizuka Y, Hirano T, Nagasaki-Maeoka E, Hoshi R, Yoshizawa S, Uekusa S, Kawashima H, Sugito K, Shinohara K, Fukuda N, Nagase H, Soma M, Koshinaga T, Fujiwara K.  ZAR1 knockdown promotes the differentiation of human neuroblastoma cells by suppression of MYCN expression.  Med. Oncol.  2017  34:158  PubMed ID: 28791558   DOI: 10.1007/s12032-017-0999-x
10480  Bingel C, Koeneke E, Ridinger J, Bittmann A, Sill M, Peterziel H, Wrobel JK, Rettig I, Milde T, Fernekorn U, Weise F, Schober A, Witt O, Oehme I.  Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance.  Cell Death Dis  2017  8:e3013  PubMed ID: 28837150   DOI: 10.1038/cddis.2017.398
7205  Indo HP, Tomiyoshi T, Suenaga S, Tomita K, Suzuki H, Masuda D, Terada M, Ishioka N, Gusev O, Cornette R, Okuda T, Mukai C, Majima HJ.  MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1.  J Clin Biochem Nutr  2015  57:98-104  PubMed ID: 26388666   DOI: 10.3164/jcbn.15-20
9499  Surasak Sangkhathat, Keigo Nara, Takeshi Kusafuka, Akihiro Yoneda, Masahiro Fukuzawa  Artificially Accumulated Beta-Catenin Inhibits Proliferation and Induces Neurite Extension of Neuroblastoma Cell Line NB-1 via Up-Regulation of trkA  Oncol Rep  2006  16(6):1197-203  PubMed ID: 17089037  
3795  Yoshio Ishii, Hiro-omi Tamura  Sulfoconjugation of Bisphenol A in a Human Neuroblastoma Cell Line, NB-1  Journal of Health Science  2003  49:319-323    DOI: 10.1248/jhs.49.319
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959   DOI: 10.1248/bpb.24.1258


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