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Cell No. : Cell Name
RCB1952 : SCCH-26  update : 2022/05/30
CommentHuman neuroblastoma cell line. TKG0460 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kaneko, Y.
Year of deposit 2004
Original cell TKG0460
Animal human < Mammals
Genus Homo
Species sapiens
Tissue unknown
Disease name neroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0460)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1686
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or (0.05% Trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 2
User's Publication 0


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Reference
5056  Hemmi H1, Yamada K, Yoon UH, Kato M, Taniguchi F, Tsuchida Y, Shimatake H.  Coexpression of the myc gene family members in human neuroblastoma cell lines.  Biochem Mol Biol Int  1995  36(6):1135-41  PubMed ID: 8535284  
3275  Kawashima I, Tada N, Fujimori T, Tai T  Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro.  J Biochem  1990  108:109-15  PubMed ID: 2121721   DOI: 10.1093/oxfordjournals.jbchem.a123148

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