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Cell No. : Cell Name
RCB1949 : TE-5  update : 2021/05/20
CommentHuman cell line derived from poorly differntiated esophageal squamous cell carcinoma. Not transplantable to nude mouse. TKG0256 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions1) In publishing the research results to be obtained by use of this cell line, an acknowledgment to the Depositor, Dr. Tetsuro Nishihira is required. 2) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Nishihira, Tetsuro
Year of deposit 2004
Original cell TKG0256
Animal human < Mammals
Genus homo
Species sapiens
Tissue esophagus
Disease name squamous carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0256)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1764
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 44


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Reference
3174  Hay, Park, Gazdar, Ed.    Atlas of Human Tumor Cell Lines  1994  269-282   
3173  Nishihira T, Hashimoto Y, Katayama M, Mori S, Kuroki T  Molecular and cellular features of esophageal cancer cells.  J Cancer Res Clin Oncol  1993  119:441-9  PubMed ID: 8509434  

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User's Publication
20614  Tsuchihashi K, Hirata Y, Yamasaki J, Suina K, Tanoue K, Yae T, Masuda K, Baba E, Akashi K, Kitagawa Y, Saya H, Nagano O.  Presence of spontaneous epithelial-mesenchymal plasticity in esophageal cancer.  Biochem Biophys Rep  2022  30:101246  PubMed ID: 35330672   DOI: 10.1016/j.bbrep.2022.101246
20046  Mitsuda M, Shiozaki A, Kudou M, Shimizu H, Arita T, Kosuga T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Otsuji E.  Functional Analysis and Clinical Significance of Chloride Channel 2 Expression in Esophageal Squamous Cell Carcinoma.  Ann Surg Oncol  2021    PubMed ID: 33565032   DOI: 10.1245/s10434-021-09659-8
20071  Matsumoto Y, Shiozaki A, Kosuga T, Kudou M, Shimizu H, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Otsuji E.  Expression and Role of CFTR in Human Esophageal Squamous Cell Carcinoma.  Ann Surg Oncol  2021    PubMed ID: 33710504   DOI: 10.1245/s10434-021-09752-y
16116  Kobayashi T, Makino T, Yamashita K, Saito T, Tanaka K, Takahashi T, Kurokawa Y, Yamasaki M, Nakajima K, Morii E, Eguchi H, Doki Y.  APR-246 induces apoptosis and enhances chemo-sensitivity via activation of ROS and TAp73-Noxa signal in oesophageal squamous cell cancer with TP53 missense mutation  Br J Cancer  2021  125(11):1523-1532  PubMed ID: 34599296   DOI: 10.1038/s41416-021-01561-0
16863  Takaki W, Konishi H, Shoda K, Arita T, Kataoka S, Shibamoto J, Furuke H, Takabatake K, Shimizu H, Komatsu S, Shiozaki A, Fujiwara H, Masuda K, Otsuji E.  Significance of Circular FAT1 as a Prognostic Factor and Tumor Suppressor for Esophageal Squamous Cell Carcinoma  Ann Surg Oncol  2021  28(13):8508-8518  PubMed ID: 34185205   DOI: 10.1245/s10434-021-10089-9
12107  Matsubara D, Konishi H, Arita T, Shoda K, Fujita Y, Ogino S, Takao K, Nanishi K, Kosuga T, Komatsu S, Shiozaki A, Fujiwara H, Okamoto K, Otsuji E.  Involvement of Intracellular and Extracellular High-Mobility Group Box-1 in the Progression of Esophageal Squamous Cell Carcinoma.  Ann. Surg. Oncol.  2020    PubMed ID: 32221734   DOI: 10.1245/s10434-020-08363-3
13092  Feng Y, Yan S, Huang Y, Huang Q, Wang F, Lei Y.  High expression of RABL6 promotes cell proliferation and predicts poor prognosis in esophageal squamous cell carcinoma.  BMC Cancer  2020    PubMed ID: 32600359   DOI: 10.1186/s12885-020-07068-w
13600  Sano H, Futamura M, Gaowa S, Kamino H, Nakamura Y, Yamaguchi K, Tanaka Y, Yasufuku I, Nakakami A, Arakawa H, Yoshida K.  p53/Mieap-regulated mitochondrial quality control plays an important role as a tumor suppressor in gastric and esophageal cancers  Biochem Biophys Res Commun  2020  529(3):582-589.  PubMed ID: 32736677   DOI: 10.1016/j.bbrc.2020.05.168
11631  Konishi T, Shiozaki A, Kosuga T, Kudou M, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Marunaka Y, Otsuji E.  LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma.  Am. J. Pathol.  2019    PubMed ID: 31323188   DOI: 10.1016/j.ajpath.2019.06.006
10798  Shinno N, Kimura H, Sada R, Takiguchi S, Mori M, Fumoto K, Doki Y, Kikuchi A.  Activation of the Dickkopf1-CKAP4 pathway is associated with poor prognosis of esophageal cancer and anti-CKAP4 antibody may be a new therapeutic drug.  Oncogene  2018  37(26):3471-3484  PubMed ID: 29563607   DOI: 10.1038/s41388-018-0179-2
11092  Shin WS, Gim J, Won S, Lee ST.  Biphasic regulation of tumorigenesis by PTK7 expression level in esophageal squamous cell carcinoma.  Sci Rep  2018  8(1):8519  PubMed ID: 29867084   DOI: 10.1038/s41598-018-26957-6
4355  Furukawa H, Makino T, Yamasaki M, Tanaka K, Miyazaki Y, Takahashi T, Kurokawa Y, Nakajima K, Takiguchi S, Mori M, Doki Y.  PRIMA-1 induces p53-mediated apoptosis by upregulating Noxa in esophageal squamous cell carcinoma with TP53 missense mutation.  Cancer Sci.  2018  109(2):412-421  PubMed ID: 29168598   DOI: 10.1111/cas.13454
4356  Ishibashi O, Akagi I, Ogawa Y, Inui T.  MiR-141-3p is upregulated in esophageal squamous cell carcinoma and targets pleckstrin homology domain leucine-rich repeat protein phosphatase-2, a negative regulator of the PI3K/AKT pathway.  Biochem Biophys Res Commun.  2018  501(2):507-513  PubMed ID: 29738771   DOI: 10.1016/j.bbrc.2018.05.025
9448  Chihiro Kajiwara, Katsumi Fumoto, Hirokazu Kimura, Satoshi Nojima, Keita Asano, Kazuki Odagiri, Makoto Yamasaki, Hayato Hikita, Tetsuo Takehara, Yuichiro Doki, Eiichi Morii, Akira Kikuchii  p63-Dependent Dickkopf3 Expression Promotes Esophageal Cancer Cell Proliferation via CKAP4  Cancer Res  2018  78(21):6107-6120  PubMed ID: 30181180   DOI: 10.1158/0008-5472.CAN-18-1749
13126  Gaowa S, Futamura M, Tsuneki M, Kamino H, Tajima JY, Mori R, Arakawa H, Yoshida K.  Possible role of p53/Mieap-regulated mitochondrial quality control as a tumor suppressor in human breast cancer.  Cancer Sci  2018    PubMed ID: 30290054   DOI: 10.1111/cas.13824
14899  Shiozaki A, Hikami S, Ichikawa D, Kosuga T, Shimizu H, Kudou M, Yamazato Y, Kobayashi T, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Marunaka Y, Otsuji E.  Anion exchanger 2 suppresses cellular movement and has prognostic significance in esophageal squamous cell carcinoma  Oncotarget  2018  9(40):25993-26006  PubMed ID: 29899837   DOI: 10.18632/oncotarget.25417
10116  Akagi I, Ishibashi O.  RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium.  Genom Data  2017  12:4-6  PubMed ID: 28224081   DOI: 10.1016/j.gdata.2017.02.002
10141  Sugase T, Takahashi T, Serada S, Nakatsuka R, Fujimoto M, Ohkawara T, Hara H, Nishigaki T, Tanaka K, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Nakajima K, Takiguchi S, Kishimoto T, Mori M, Doki Y, Naka T.  Suppressor of cytokine signaling-1 gene therapy induces potent antitumor effect in patient-derived esophageal squamous cell carcinoma xenograft mice.  Int. J. Cancer  2017  140:2608-2621  PubMed ID: 28233302   DOI: 10.1002/ijc.30666
10323  Liu K, Song G, Zhang X, Li Q, Zhao Y, Zhou Y, Xiong R, Hu X, Tang Z, Feng G.  PTK7 is a novel oncogenic target for esophageal squamous cell carcinoma.  World J Surg Oncol  2017  15:105  PubMed ID: 28545451   DOI: 10.1186/s12957-017-1172-x
10353  Kawaguchi T, Komatsu S, Ichikawa D, Hirajima S, Nishimura Y, Konishi H, Shiozaki A, Fujiwara H, Okamoto K, Tsuda H, Otsuji E.  Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma.  Tumour Biol.  2017  39:1010428317700409  PubMed ID: 28618928   DOI: 10.1177/1010428317700409
10365  Yamamoto M, Yamasaki M, Tsukao Y, Tanaka K, Miyazaki Y, Makino T, Takahashi T, Kurokawa Y, Nakajima K, Takiguchi S, Mori M, Doki Y.  Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma.  Oncol Lett  2017  14:1581-1587  PubMed ID: 28789382   DOI: 10.3892/ol.2017.6334
10449  Yoshioka M, Ohashi S, Ida T, Nakai Y, Kikuchi O, Amanuma Y, Matsubara J, Yamada A, Miyamoto S, Natsuizaka M, Nakagawa H, Chiba T, Seno H, Muto M.  Distinct effects of EGFR inhibitors on epithelial- and mesenchymal-like esophageal squamous cell carcinoma cells.  J. Exp. Clin. Cancer Res.  2017  36:101  PubMed ID: 28764725   DOI: 10.1186/s13046-017-0572-7
14228  Fujita Y, Masuda K, Hamada J, Shoda K, Naruto T, Hamada S, Miyakami Y, Kohmoto T, Watanabe M, Takahashi R, Tange S, Saito M, Kudo Y, Fujiwara H, Ichikawa D, Tangoku A, Otsuji E, Imoto I.  KH-type splicing regulatory protein is involved in esophageal squamous cell carcinoma progression  Oncotarget   2017  ;8(60):101130-101145.  PubMed ID: 29254151   DOI: 10.18632/oncotarget.20926
14243  Shiozaki A, Kudou M, Ichikawa D, Shimizu H, Arita T, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.  Expression and role of anion exchanger 1 in esophageal squamous cell carcinoma  Oncotarget  2017  8(11):17921-17935  PubMed ID: 28160546   DOI: 10.18632/oncotarget.14900
9599  Kikuchi O, Ohashi S, Horibe T, Kohno M, Nakai Y, Miyamoto S, Chiba T, Muto M, Kawakami K.  Novel EGFR-targeted strategy with hybrid peptide against oesophageal squamous cell carcinoma.  Sci Rep  2016  6:22452  PubMed ID: 26956916   DOI: 10.1038/srep22452
9724  Hara H, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Nakatsuka R, Harada E, Nishigaki T, Takahashi Y, Nojima S, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Miyata H, Nakajima K, Takiguchi S, Morii E, Mori M, Doki Y, Naka T.  Overexpression of glypican-1 implicates poor prognosis and their chemoresistance in oesophageal squamous cell carcinoma.  Br. J. Cancer  2016  115:66-75  PubMed ID: 27310703   DOI: 10.1038/bjc.2016.183
9433  Yunyan Wu, Qiang Liu, Xu Yan, Yukio Kato, Makiko Tanaka, Sadaki Inokuchi, Tadashi Yoshizawa, Satoko Morohashi, Hiroshi Kijima  Podoplanin-mediated TGF-β-induced Epithelial-Mesenchymal Transition and Its Correlation With bHLH Transcription Factor DEC in TE-11 Cells  Int J Oncol  2016  48(6):2310-20  PubMed ID: 27035755   DOI: 10.3892/ijo.2016.3445
12663  Song IH, Lee TS, Park YS, Lee JS, Lee BC, Moon BS, An GI, Lee HW, Kim KI, Lee YJ, Kang JH, Lim SM.  Immuno-PET Imaging and Radioimmunotherapy of 64Cu-/177Lu-Labeled Anti-EGFR Antibody in Esophageal Squamous Cell Carcinoma Model  J Nucl Med  2016  57(7):1105-11  PubMed ID: 26917708   DOI: 10.2967/jnumed.115.167155
12664  Shin WS, Hong Y, Lee HW, Lee ST.  Catalytically defective receptor protein tyrosine kinase PTK7 enhances invasive phenotype by inducing MMP-9 through activation of AP-1 and NF-κB in esophageal squamous cell carcinoma cells  Oncotarget  2016  7(45):73242-73256  PubMed ID: 27689325   DOI: 10.18632/oncotarget.12303
12969  Hamada J, Shoda K, Masuda K, Fujita Y, Naruto T, Kohmoto T, Miyakami Y, Watanabe M, Kudo Y, Fujiwara H, Ichikawa D, Otsuji E, Imoto I.  Tumor-promoting function and prognostic significance of the RNA-binding protein T-cell intracellular antigen-1 in esophageal squamous cell carcinoma  Oncotarget  2016  7(13):17111-28  PubMed ID: 26958940   DOI: 10.18632/oncotarget.7937
14119  Park M, Yoon HJ, Kang MC, Kwon J, Lee HW.  PTK7 regulates radioresistance through nuclear factor-kappa B in esophageal squamous cell carcinoma  Tumour Biol  2016  37(10):14217-14224  PubMed ID: 27557627   DOI: 10.1007/s13277-016-5288-3
7202  Kikuchi O, Ohashi S, Nakai Y, Nakagawa S, Matsuoka K, Kobunai T, Takechi T, Amanuma Y, Yoshioka M, Ida T, Yamamoto Y, Okuno Y, Miyamoto S, Nakagawa H, Matsubara K, Chiba T, Muto M.  Novel 5-fluorouracil-resistant human esophageal squamous cell carcinoma cells with dihydropyrimidine dehydrogenase overexpression.  Am J Cancer Res  2015  5:2431-40  PubMed ID: 26396918  
7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729   DOI: 10.1186/s12885-015-1065-8
7493  Wakita A, Motoyama S, Sato Y, Koyota S, Usami S, Yoshino K, Sasaki T, Imai K, Saito H, Minamiya Y.  REG Iα activates c-Jun through MAPK pathways to enhance the radiosensitivity of squamous esophageal cancer cells.  Tumour Biol.  2015  36:5249-54  PubMed ID: 25656613   DOI: 10.1007/s13277-015-3183-y
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144   DOI: 10.1073/pnas.1510077112
15822  Ohashi S, Kikuchi O, Tsurumaki M, Nakai Y, Kasai H, Horimatsu T, Miyamoto S, Shimizu A, Chiba T, Muto M.  Preclinical validation of talaporfin sodium-mediated photodynamic therapy for esophageal squamous cell carcinoma  PLoS One  2014  9(8):e103126  PubMed ID: 25090101   DOI: 10.1371/journal.pone.0103126
15825  Inhibition of mitogen-activated protein kinase pathway can induce upregulation of human leukocyte antigen class I without PD-L1-upregulation in contrast to interferon-γ treatment  Inhibition of mitogen-activated protein kinase pathway can induce upregulation of human leukocyte antigen class I without PD-L1-upregulation in contrast to interferon-γ treatment  Cancer Sci  2014  105(10):1236-44  PubMed ID: 25154680   DOI: 10.1111/cas.12503
16011  Zhang S, Tang W, Weng S, Liu X, Rao B, Gu J, Chen S, Wang Q, Shen X, Xue R, Dong L.  Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma  Oncotarget  2014  5(16):7183-97  PubMed ID: 25216531   DOI: 10.18632/oncotarget.2293
16019  Yoshino K, Motoyama S, Koyota S, Shibuya K, Sato Y, Sasaki T, Wakita A, Saito H, Minamiya Y, Sugiyama T, Ogawa J.  Identification of insulin-like growth factor 2 mRNA-binding protein 3 as a radioresistance factor in squamous esophageal cancer cells  Dis Esophagus  2014  27(5):479-84  PubMed ID: 22989274   DOI: 10.1111/j.1442-2050.2012.01415.x
17149  Yeo SY, Ha SY, Yu EJ, Lee KW, Kim JH, Kim SH.  ZNF282 (Zinc finger protein 282), a novel E2F1 co-activator, pr  Oncotarget  2014  5(23):12260-72  PubMed ID: 25373738   DOI: 10.18632/oncotarget.2630
12745  Matsushima K, Isomoto H, Yamaguchi N, Inoue N, Machida H, Nakayama T, Hayashi T, Kunizaki M, Hidaka S, Nagayasu T, Nakashima M, Ujifuku K, Mitsutake N, Ohtsuru A, Yamashita S, Korpal M, Kang Y, Gregory PA, Goodall GJ, Kohno S, Nakao K.  MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells  J Transl Med  2011  9:30  PubMed ID: 21426561   DOI: 10.1186/1479-5876-9-30
8377  Usami S, Motoyama S, Koyota S, Wang J, Hayashi-Shibuya K, Maruyama K, Takahashi N, Saito H, Minamiya Y, Takasawa S, Ogawa J, Sugiyama T.  Regenerating gene I regulates interleukin-6 production in squamous esophageal cancer cells.  Biochem. Biophys. Res. Commun.  2010  392:4-8  PubMed ID: 20056108   DOI: 10.1016/j.bbrc.2009.12.129
8430  Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M.  Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma.  Int. J. Cancer  2010  127:543-54  PubMed ID: 19960436   DOI: 10.1002/ijc.25082
12755  Inoue N, Isomoto H, Matsushima K, Hayashi T, Kunizaki M, Hidaka S, Machida H, Mitsutake N, Nanashima A, Takeshima F, Nakayama T, Ohtsuru A, Nakashima M, Nagayasu T, Yamashita S, Nakao K, Kohno S.  Down-regulation of microRNA 10a expression in esophageal squamous cell carcinoma cells  Oncol Lett  2010  1(3):527-531  PubMed ID: 22966337   DOI: 10.3892/ol_00000093



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