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Cell No. : Cell Name
RCB1943 : HuH-28  update : 2020/09/24
CommentHuman cell line derived from cholangiocellular carcinoma. TKG0438 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Kusaka, Y.
Year of deposit 2004
Original cell TKG0438
Animal human < Mammals
Genus Homo
Species sapiens
Tissue liver
Case history cholangiocellular carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG00438)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Reference information Reference 2
User's Publication 6

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5051  Kusaka Y1, Muraoka A, Tokiwa T, Sato J.  Establishment and characterization of a human cholangiocellular carcinoma cell line  Hum Cell  1988  1(1):92-4  PubMed ID: 2856443
5052  Kusaka Y1, Tokiwa T, Sato J.  Establishment and characterization of a cell line from a human cholangiocellular carcinoma.  Res Exp Med (Berl)  1988  188(5):367-75  PubMed ID: 2852388

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User's Publication
10740  Merdrignac A, Angenard G, Allain C, Petitjean K, Bergeat D, Bellaud P, Fautrel A, Turlin B, Clément B, Dooley S, Sulpice L, Boudjema K, Coulouarn C.  A novel transforming growth factor beta-induced long noncoding RNA promotes an inflammatory microenvironment in human intrahepatic cholangiocarcinoma.  Hepatol Commun  2018  2:254-269  PubMed ID: 29507901
10770  Mao Y, Huang X, Shuang Z, Lin G, Wang J, Duan F, Chen J, Li S.  PARP inhibitor olaparib sensitizes cholangiocarcinoma cells to radiation.  Cancer Med  2018  7:1285-1296  PubMed ID: 29479816
4487  Shuang Z, Mao Y, Lin G, Wang J, Huang X, Chen J, Duan F, Li S.  Alpha-L-Fucosidase Serves as a Prognostic Indicator for Intrahepatic Cholangiocarcinoma and Inhibits Its Invasion Capacity.  iomed Res Int  2018  2018:8182575  PubMed ID: 29682557
10055  Tan X, Huang Z, Li X.  Long Non-Coding RNA MALAT1 Interacts With miR-204 to Modulate Human Hilar Cholangiocarcinoma Proliferation, Migration, and Invasion by Targeting CXCR4.  J. Cell. Biochem.  2017  118:3643-3653  PubMed ID: 28059437
3722  Ohashi H, Adachi Y, Yamamoto H, Taniguchi H, Nosho K, Suzuki H, Arimura Y, Imai K, Carbone DP, Shinomura Y.  Insulin-like growth factor receptor expression is associated with aggressive phenotypes and has therapeutic activity in biliary tract cancers.  Cancer Sci  2012  103(2):252-61  PubMed ID: 22044563
7938  Sugiyama H, Onuki K, Ishige K, Baba N, Ueda T, Matsuda S, Takeuchi K, Onodera M, Nakanuma Y, Yamato M, Yamamoto M, Hyodo I, Shoda J.  Potent in vitro and in vivo antitumor activity of sorafenib against human intrahepatic cholangiocarcinoma cells.  J. Gastroenterol.  2011  46:779-89  PubMed ID: 21331764

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