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Cell No. : Cell Name
RCB1943 : HuH-28  update : 2022/11/01
CommentHuman cell line derived from cholangiocellular carcinoma. TKG0438 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kusaka, Y.
Year of deposit 2004
Original cell TKG0438
Animal human < Mammals
Genus Homo
Species sapiens
Tissue liver
Disease name cholangiocellular carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG00438)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2955
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 2
User's Publication 21


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Reference
5051  Kusaka Y1, Muraoka A, Tokiwa T, Sato J.  Establishment and characterization of a human cholangiocellular carcinoma cell line  Hum Cell  1988  1(1):92-4  PubMed ID: 2856443  
5052  Kusaka Y1, Tokiwa T, Sato J.  Establishment and characterization of a cell line from a human cholangiocellular carcinoma.  Res Exp Med (Berl)  1988  188(5):367-75  PubMed ID: 2852388   DOI: 10.1007/bf01851205

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User's Publication
15892  Khorsandi SE, Dokal AD, Rajeeve V, Britton DJ, Illingworth MS, Heaton N, Cutillas PR.  Computational Analysis of Cholangiocarcinoma Phosphoproteomes Identifies Patient-Specific Drug Targets.  Cancer Res  2021  81(22):5765-5776  PubMed ID: 34551960   DOI: 10.1158/0008-5472.CAN-21-0955
12589  Miyata T, Yamashita YI, Yoshizumi T, Shiraishi M, Ohta M, Eguchi S, Aishima S, Fujioka H, Baba H.  CXCL12 expression in intrahepatic cholangiocarcinoma is associated with metastasis and poor prognosis  Cancer Sci   2019  110(10):3197-3203  PubMed ID: 31361379   DOI: 10.1111/cas.14151
14816  Goyal L, Shi L, Liu LY, Fece de la Cruz F, Lennerz JK, Raghavan S, Leschiner I, Elagina L, Siravegna G, Ng RWS, Vu P, Patra KC, Saha SK, Uppot RN, Arellano R, Reyes S, Sagara T, Otsuki S, Nadres B, Shahzade HA, Dey-Guha I, Fetter IJ, Baiev I, Van Seventer EE, Murphy JE, Ferrone CR, Tanabe KK, Deshpande V, Harding JJ, Yaeger R, Kelley RK, Bardelli A, Iafrate AJ, Hahn WC, Benes CH, Ting DT, Hirai H, Getz G, Juric D, Zhu AX, Corcoran RB, Bardeesy N.  TAS-120 Overcomes Resistance to ATP-Competitive FGFR Inhibitors in Patients with FGFR2 Fusion-Positive Intrahepatic Cholangiocarcinoma  Cancer Discov  2019  9(8):1064-1079  PubMed ID: 31109923   DOI: 10.1158/2159-8290.CD-19-0182
10740  Merdrignac A, Angenard G, Allain C, Petitjean K, Bergeat D, Bellaud P, Fautrel A, Turlin B, Clément B, Dooley S, Sulpice L, Boudjema K, Coulouarn C.  A novel transforming growth factor beta-induced long noncoding RNA promotes an inflammatory microenvironment in human intrahepatic cholangiocarcinoma.  Hepatol Commun  2018  2:254-269  PubMed ID: 29507901   DOI: 10.1002/hep4.1142
10770  Mao Y, Huang X, Shuang Z, Lin G, Wang J, Duan F, Chen J, Li S.  PARP inhibitor olaparib sensitizes cholangiocarcinoma cells to radiation.  Cancer Med  2018  7:1285-1296  PubMed ID: 29479816   DOI: 10.1002/cam4.1318
4487  Shuang Z, Mao Y, Lin G, Wang J, Huang X, Chen J, Duan F, Li S.  Alpha-L-Fucosidase Serves as a Prognostic Indicator for Intrahepatic Cholangiocarcinoma and Inhibits Its Invasion Capacity.  iomed Res Int  2018  2018:8182575  PubMed ID: 29682557   DOI: 10.1155/2018/8182575
10055  Tan X, Huang Z, Li X.  Long Non-Coding RNA MALAT1 Interacts With miR-204 to Modulate Human Hilar Cholangiocarcinoma Proliferation, Migration, and Invasion by Targeting CXCR4.  J. Cell. Biochem.  2017  118:3643-3653  PubMed ID: 28059437   DOI: 10.1002/jcb.25862
10501  Kushibiki T, Noji T, Ebihara Y, Hontani K, Ono M, Kuwabara S, Nakamura T, Tsuchikawa T, Okamura K, Ishizuka M, Hirano S.  5-Aminolevulinic-acid-mediated Photodynamic Diagnosis Enhances the Detection of Peritoneal Metastases in Biliary Tract Cancer in Mice.  In Vivo  2017  31:905-908  PubMed ID: 28882957   DOI: 10.21873/invivo.11145
14252  Maki T, Ikeda H, Kuroda A, Kyogoku N, Yamamura Y, Tabata Y, Abiko T, Tsuchikawa T, Hida Y, Shichinohe T, Tanaka E, Kaga K, Hatanaka K, Matsuno Y, Imai N, Hirano S.  Differential detection of cytoplasmic Wilms tumor 1 expression by immunohistochemistry, western blotting and mRNA quantification  Int J Oncol  2017  50(1):129-140.  PubMed ID: 27922671   DOI: 10.3892/ijo.2016.3786
14271  Muguruma N, Okamoto K, Nakagawa T, Sannomiya K, Fujimoto S, Mitsui Y, Kimura T, Miyamoto H, Higashijima J, Shimada M, Horino Y, Matsumoto S, Hanaoka K, Nagano T, Shibutani M, Takayama T.  Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1  Sci Rep  2017  7(1):6536  PubMed ID: 28747791   DOI: 10.1038/s41598-017-06857-x
14528  Yagai T, Matsui S, Harada K, Inagaki FF, Saijou E, Miura Y, Nakanuma Y, Miyajima A, Tanaka M.  Expression and localization of sterile alpha motif domain containing 5 is associated with cell type and malignancy of biliary tree  PLoS One  2017  12(4):e0175355  PubMed ID: 28388653   DOI: 10.1371/journal.pone.0175355
12673  Cavalloni G, Peraldo-Neia C, Varamo C, Casorzo L, Dell'Aglio C, Bernabei P, Chiorino G, Aglietta M, Leone F.  Establishment and characterization of a human intrahepatic cholangiocarcinoma cell line derived from an Italian patient  Tumour Biol   2016  37(3):4041-52  PubMed ID: 26486326   DOI: 10.1007/s13277-015-4215-3
12878  Yang SH, Lin HY, Changou CA, Chen CH, Liu YR, Wang J, Jiang X, Luh F, Yen Y.  Integrin β3 and LKB1 are independently involved in the inhibition of proliferation by lovastatin in human intrahepatic cholangiocarcinoma  Oncotarget  2016  7(1):362-73  PubMed ID: 26517522   DOI: 10.18632/oncotarget.6238
14116  Huyen NT, Prachayasittikul V, Chan-On W.  Anoikis-resistant cholangiocarcinoma cells display aggressive characteristics and increase STAT3 activation  J Hepatobiliary Pancreat Sci  2016  23(7):397-405  PubMed ID: 27107220   DOI: 10.1002/jhbp.354
9170  Sheng-Huei Yang 1, Hung-Yun Lin, Vincent H S Chang, Chien-Chung Chen, Yun-Ru Liu, Jinghan Wang, Keqiang Zhang, Xiaoqing Jiang, Yun Yen  Lovastatin Overcomes Gefitinib Resistance Through TNF-α Signaling in Human Cholangiocarcinomas With Different LKB1 Statuses in Vitro and in Vivo  Oncotarget  2015  6 (27), 23857-73  PubMed ID: 26160843   DOI: 10.18632/oncotarget.4408
14311  Chan-On W, Huyen NT, Songtawee N, Suwanjang W, Prachayasittikul S, Prachayasittikul V.  Quinoline-based clioquinol and nitroxoline exhibit anticancer activity inducing FoxM1 inhibition in cholangiocarcinoma cells  Drug Des Devel Ther  2015  9:2033-47  PubMed ID: 25897210   DOI: 10.2147/DDDT.S79313
15203  Wang J, Zhang K, Wang J, Wu X, Liu X, Li B, Zhu Y, Yu Y, Cheng Q, Hu Z, Guo C, Hu S, Mu B, Tsai CH, Li J, Smith L, Yang L, Liu Q, Chu P, Chang V, Zhang B, Wu M, Jiang X, Yen Y.  Underexpression of LKB1 tumor suppressor is associated with enhanced Wnt signaling and malignant characteristics of human intrahepatic cholangiocarcinoma  Oncotarget  2015  6(22):18905-20  PubMed ID: 26056085   DOI: 10.18632/oncotarget.4305
14930  Takahashi N, Aoyama F, Hiyoshi M, Kataoka H, Sawaguchi A.  Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater  Int J Oncol  2014  44(4):1139-45  PubMed ID: 24481592   DOI: 10.3892/ijo.2014.2282
3722  Ohashi H, Adachi Y, Yamamoto H, Taniguchi H, Nosho K, Suzuki H, Arimura Y, Imai K, Carbone DP, Shinomura Y.  Insulin-like growth factor receptor expression is associated with aggressive phenotypes and has therapeutic activity in biliary tract cancers.  Cancer Sci  2012  103(2):252-61  PubMed ID: 22044563   DOI: 10.1111/j.1349-7006.2011.02138.x
7938  Sugiyama H, Onuki K, Ishige K, Baba N, Ueda T, Matsuda S, Takeuchi K, Onodera M, Nakanuma Y, Yamato M, Yamamoto M, Hyodo I, Shoda J.  Potent in vitro and in vivo antitumor activity of sorafenib against human intrahepatic cholangiocarcinoma cells.  J. Gastroenterol.  2011  46:779-89  PubMed ID: 21331764   DOI: 10.1007/s00535-011-0380-3
15724  Araki K, Shimura T, Suzuki H, Tsutsumi S, Wada W, Yajima T, Kobayahi T, Kubo N, Kuwano H.  E/N-cadherin switch mediates cancer progression via TGF-β-induced epithelial-to-mesenchymal transition in extrahepatic cholangiocarcinoma  Br J Cancer  2011  105(12):1885-93  PubMed ID: 22068819   DOI: 10.1038/bjc.2011.452



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