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Cell No. : Cell Name
RCB1942 : HuH-7  update : 2020/08/14
CommentHuman cell line derived from hepatoma. TKG0206 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Nakabayashi, H.
Year of deposit 2004
Original cell TKG0206
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 57 years
Tissue liver
Case history Hepatoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0206)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Virus (HBV) (-)
Virus (HCV) (-)
deposit info
lot info
Reference information Reference 0
User's Publication 7

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User's Publication
9193  Said M Afify, Anna Sanchez Calle, Ghmkin Hassan, Kazuki Kumon, Hend M Nawara, Maram H Zahra, Hager M Mansour, Apriliana Cahya Khayrani, Md Jahangir Alam, Juan Du, Akimasa Seno, Yoshiaki Iwasaki, Masaharu Seno   A Novel Model of Liver Cancer Stem Cells Developed From Induced Pluripotent Stem Cells  Br J Cancer  2020  122(9):1378-1390  PubMed ID: 32203212
4225  Takashima Y, Horisawa K, Udono M, Ohkawa Y, Suzuki A.  Prolonged inhibition of hepatocellular carcinoma cell proliferation by combinatorial expression of defined transcription factors.  Cancer Sci.  2018    PubMed ID: 30220099
4255  Ono K, Igata M, Kondo T, Kitano S, Takaki Y, Hanatani S, Sakaguchi M, Goto R, Senokuchi T, Kawashima J, Furukawa N, Motoshima H, Araki E.  Identification of microRNA that represses IRS-1 expression in liver.  PLoS ONE  2018    PubMed ID: 29364977
10257  Ruan HY, Yang C, Tao XM, He J, Wang T, Wang H, Wang C, Jin GZ, Jin HJ, Qin WX.  Downregulation of ACSM3 promotes metastasis and predicts poor prognosis in hepatocellular carcinoma.  Am J Cancer Res  2017  7:543-553  PubMed ID: 28401010
6403  Terasaka N, Futai K, Katoh T, Suga H.  A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX.  RNA  2016  22(12):1918-1928  PubMed ID: 27852928
7422  Zhang Y, Luo Q, Wang N, Hu F, Jin H, Ge T, Wang C, Qin W.  LRG1 suppresses the migration and invasion of hepatocellular carcinoma cells.  Med. Oncol.  2015  32:146  PubMed ID: 25814288
6531  Kan A, Ikeda T, Saito T, Yano F, Fukai A, Hojo H, Ogasawara T, Ogata N, Nakamura K, Chung UI, Kawaguchi H.  Screening of chondrogenic factors with a real-time fluorescence-monitoring cell line ATDC5-C2ER: identification of sorting nexin 19 as a novel factor.  Arthritis Rheum  2009  60(11):3314-23  PubMed ID: 19877062

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