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Cell No. : Cell Name
RCB1941 : Li-7  update : 2018/08/01
CommentHuman hepatoma cell line. This cell line was established from nude mouse xenograft tumor (Dr. Hirohashi., National Cancer Center, Tokyo) by Tanno, H. (Tohoku Univ., 1st Surg). AFP producing. TKG0368 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Tanno, H. (Tohoku Univ., 1st Surg)
Year of deposit 2004
Original cell TKG0368
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 45 years
Tissue liver
Case history Hepatoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0368)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method (0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 57-64(50) : 57(8),58(9),59(11),60(2),61(3),62(12)/
Images
deposit info
lot info
Reference information Reference 0
User's Publication 7


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Reference

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User's Publication
4276  Fujisaka Y, Iwata T, Tamai K, Nakamura M, Mochizuki M, Shibuya R, Yamaguchi K, Shimosegawa T, Satoh K.  Long non-coding RNA HOTAIR up-regulates chemokine (C-C motif) ligand 2 and promotes proliferation of macrophages and myeloid-derived suppressor cells in hepatocellular carcinoma cell lines.  Oncol Lett  2018    PubMed ID: 29387231
5802  Eguchi T et al  Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment.  PLoS One  2018  13(2):e0191109.  PubMed ID: 29415026
10240  Ohata T, Yokoo H, Kamiyama T, Fukai M, Aiyama T, Hatanaka Y, Hatanaka K, Wakayama K, Orimo T, Kakisaka T, Kobayashi N, Matsuno Y, Taketomi A.  Fatty acid-binding protein 5 function in hepatocellular carcinoma through induction of epithelial-mesenchymal transition.  Cancer Med  2017  6:1049-1061  PubMed ID: 28374947
9574  Ferraris P, Chandra PK, Panigrahi R, Aboulnasr F, Chava S, Kurt R, Pawlotsky JM, Wilkens L, Osterlund P, Hartmann R, Balart LA, Wu T, Dash S.  Cellular Mechanism for Impaired Hepatitis C Virus Clearance by Interferon Associated with IFNL3 Gene Polymorphisms Relates to Intrahepatic Interferon-λ Expression.  Am. J. Pathol.  2016  186:938-51  PubMed ID: 26896692
9576  Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T.  Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate.  Int. J. Oncol.  2016  48:1794-804  PubMed ID: 26936487
5863  Yoshii Y, Waki A, Yoshida K, Kakezuka A, Kobayashi M, Namiki H, Kuroda Y, Kiyono Y, Yoshii H, Furukawa T, Asai T, Okazawa H, Gelovani JG, Fujibayashi Y.  The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation.  Biomaterials  2011  32(26):6052-8  PubMed ID: 21640378
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309



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