Cell No. : Cell Name
RCB1941 : Li-7
update : 2018/08/01
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| Comment | Human hepatoma cell line. This cell line was established from nude mouse xenograft tumor (Dr. Hirohashi., National Cancer Center, Tokyo) by Tanno, H. (Tohoku Univ., 1st Surg). AFP producing. TKG0368 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | Basically, there is no restriction regarding academic use. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Obinata, Masuo
|
| Originator |
Tanno, H. (Tohoku Univ., 1st Surg)
|
| Year of deposit |
2004
|
| Original cell |
TKG0368
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
45 years
|
| Tissue |
liver
|
| Case history |
Hepatoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0368）
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Medium and additives |
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RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
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(0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
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Culture information
|
Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
|
| Mycoplasma |
|
(-)
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| STR(human) |
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OK
|
| Virus (HBV) |
|
(-)
|
| Virus (HCV) |
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(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
57-64(50) : 57(8),58(9),59(11),60(2),61(3),62(12)/
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
|
| User's Publication |
7
|
| User's Publication |
|
4276
Fujisaka Y, Iwata T, Tamai K, Nakamura M, Mochizuki M, Shibuya R, Yamaguchi K, Shimosegawa T, Satoh K.
Long non-coding RNA HOTAIR up-regulates chemokine (C-C motif) ligand 2 and promotes proliferation of macrophages and myeloid-derived suppressor cells in hepatocellular carcinoma cell lines.
Oncol Lett
2018
PubMed ID: 29387231
|
|
5802
Eguchi T et al
Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment.
PLoS One
2018
13(2):e0191109.
PubMed ID: 29415026
|
|
10240
Ohata T, Yokoo H, Kamiyama T, Fukai M, Aiyama T, Hatanaka Y, Hatanaka K, Wakayama K, Orimo T, Kakisaka T, Kobayashi N, Matsuno Y, Taketomi A.
Fatty acid-binding protein 5 function in hepatocellular carcinoma through induction of epithelial-mesenchymal transition.
Cancer Med
2017
6:1049-1061
PubMed ID: 28374947
|
|
9574
Ferraris P, Chandra PK, Panigrahi R, Aboulnasr F, Chava S, Kurt R, Pawlotsky JM, Wilkens L, Osterlund P, Hartmann R, Balart LA, Wu T, Dash S.
Cellular Mechanism for Impaired Hepatitis C Virus Clearance by Interferon Associated with IFNL3 Gene Polymorphisms Relates to Intrahepatic Interferon-λ Expression.
Am. J. Pathol.
2016
186:938-51
PubMed ID: 26896692
|
|
9576
Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T.
Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate.
Int. J. Oncol.
2016
48:1794-804
PubMed ID: 26936487
|
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5863
Yoshii Y, Waki A, Yoshida K, Kakezuka A, Kobayashi M, Namiki H, Kuroda Y, Kiyono Y, Yoshii H, Furukawa T, Asai T, Okazawa H, Gelovani JG, Fujibayashi Y.
The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation.
Biomaterials
2011
32(26):6052-8
PubMed ID: 21640378
|
|
6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
|
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