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Cell No. : Cell Name
RCB1938 : P30/OHK  update : 2022/08/12
CommentHuman cell line derived from T cell leukemia. TKG0463 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Hirose, M.
Year of deposit 2004
Original cell TKG0463
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 11 years
Tissue Peripheral blood
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0463)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1631
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
5048  Hirose M, Minato K, Tobinai K, Ohira M, Ise T, Watanabe S, Shimoyama M, Taniwaki M, Abe T.  A novel pre-T cell line derived from acute lymphoblastic leukemia.  Gan  1982  73(4):600-5  PubMed ID: 6983990

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User's Publication



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