Cell No. : Cell Name
RCB1936 : MOLT-4F
update : 2024/08/14
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| Comment | Human T leukemic cell line (CD4+). CR2 receptor (+). TKG0229 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Obinata, Masuo
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| Originator |
Minowada, J.
|
| Year of deposit |
2004
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| Original cell |
TKG0229
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Male
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| Age at sampling |
19 years
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| Tissue |
blood
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| Disease name |
T cell leukemia
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| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0229）
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_2792
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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LT 400
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| Virus (HTLV-1) |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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93-100(50) : /94(5),95(5),96(15),97(14),98(5),99(2)
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| STR(human) |
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OK
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| Reference information |
Reference |
12
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| User's Publication |
2
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| Reference |
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4748
Nakazawa S, Minowada J, Tsubota T, Sinks LF.
Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling.
Cancer Res
1978
38(6):1661-6
PubMed ID: 306283
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4747
Han T, Dadey B, Minowada J.
Stimulating capacity of fresh and cultured human leukaemic lymphoid and myeloid cells in 'one-way' mixed lymphocyte reaction.
Immunology
1977
33(4):543-51
PubMed ID: 144702
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4746
Cohen E, Minowada J, Pliss M, Pliss L, Blumenson LE.
Differentiation of human leukemic from normal lymphocytes by Limulus serum agglutination.
Vox Sang
1976
31(2):117-23
PubMed ID: 1085067
DOI: 10.1111/j.1423-0410.1976.tb02197.x
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4745
Pauly JL, Minowada J, Han T, Moore GE.
Disparity of mixed lymphocyte reactivity to cultured cells of human T and B lymphoid lines.
J Natl Cancer Inst
1975
54(3):557-62.
PubMed ID: 123598
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160
Hayata I, Oshimura M, Minowada J, Sandberg AA.
Chromosomal banding of cultured T and B lymphocytes
In Vitro
1975
11(6):361-8.
PubMed ID: 1081484
DOI: 10.1007/BF02616372
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4743
Itoh U, Minowada J, Moore GE, Pressman D.
Rosette-forming human lymphoid cell line (T-cell line molt). II. Ability for clonal growth.
J Natl Cancer Inst
1974
52(5):1403-7
PubMed ID: 4545482
DOI: 10.1093/jnci/52.5.1403
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4744
Takada A, Takada Y, Minowada J.
Immunological functions of human T-lymphoid cell line (MOLT). I. Release of immunosuppressive factors from the mixture of MOLT-4 cells and sheep red blood cells.
J Exp Med
1974
140(2):538-48
PubMed ID: 4603015
DOI: 10.1084/jem.140.2.538
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159
Huang CC, Hou Y, Woods LK, Moore GE, Minowada J.
Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia
J Natl Cancer Inst
1974
53(3):655-60
PubMed ID: 4547172
DOI: 10.1093/jnci/53.3.655
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4740
Sahai Srivastava BI, Minowada J.
Terminal deoxynucleotidyl transferase activity in a cell line (molt-4) derived from the peripheral blood of a patient with acute lymphoblastic leukemia.
Biochem Biophys Res Commun
1973
51(3):529-35
PubMed ID: 4512981
DOI: 10.1016/0006-291x(73)91346-6
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4741
Gailani S, Minowada J, Silvernail P, Nussbaum A, Kaiser N, Rosen F, Shimaoka K.
Specific glucocorticoid binding in human hemopoietic cell lines and neoplastic tissue.
Cancer Res
1973
33(11):2653-7
PubMed ID: 4355977
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4742
Han T, Minowada J.
A unique 'leukaemic' T lymphoid cell line: absence of stimulating effect in mixed lymphocyte reaction. Lack of MLR-S in leukaemic T lymphoid cells.
Clin Exp Immunol
1973
15(4):535-41
PubMed ID: 4273215
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494
Minowada J, Onuma T, Moore, G E
Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
J Natl Cancer Inst
1972
49:891-5
PubMed ID: 4567231
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| User's Publication |
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17656
Ryo C. Yanagita, Mao Otani, Satoshi Hatanaka, Hiroto Nishi, Shota Miyake, Yusuke Hanaki, Masashi Sato, Yasuhiro Kawanami, Kazuhiro Irie
Analysis of binding mode of vibsanin A with protein kinase C C1 domains: An experimental and molecular dynamics simulation study
Journal of Molecular Structure
2022
1260
DOI: 10.1016/j.molstruc.2022.132866
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11709
Hironobu Ishiyama, Ryo C. Yanagita, Kazune Takemoto, Katsuya Kobashi, Yasunori Sugiyama, Yasuhiro Kawanami
Development of a d-allose-6-phosphate derivative with anti-proliferative activity against a human leukemia MOLT-4F cell line
Carbohydrate Research
2020
487:107859
PubMed ID: 31751780
DOI: 10.1016/j.carres.2019.107859
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