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Cell No. : Cell Name
RCB1935 : HPB-ALL  update : 2021/05/20
CommentHuman cell line derived from T cell leukemia. TKG0199 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0199
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue blood
Disease name T cell leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0199)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1820
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 2
User's Publication 4


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Reference
5071  Drexler HG, Gaedicke G, Minowada J.  T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.  Blut  1987  54(2):79-87  PubMed ID: 3493047
5047  Morikawa S, Tatsumi E, Baba M, Harada T, Yasuhira K.  Two E-rosette-forming lymphoid cell lines.  Int J Cancer  1978  21(2):166-70  PubMed ID: 203546

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User's Publication
20393  Makita Y, Saito S, Tsuchiya A, Ishibashi M, Arai MA.  Identification of 1β,2α-epoxytagitinin C as a Notch inhibitor, oxidative stress mechanism and its anti-leukemia activity.  J Nat Med  2022    PubMed ID: 34779991
13112  Tsuchiya A, Makita Y, Koyano T, Kowithayakorn T, Ishibashi M, Arai MA.  Isolation and evaluation of cardenolides from Lansium domesticum as Notch inhibitors.  J Nat Med  2020    PubMed ID: 32648094
10804  Arai MA, Akamine R, Tsuchiya A, Yoneyama T, Koyano T, Kowithayakorn T, Ishibashi M.  The Notch inhibitor cowanin accelerates nicastrin degradation.  Sci Rep  2018  8:5376  PubMed ID: 29599482
9651  Randhawa S, Cho BS, Ghosh D, Sivina M, Koehrer S, Müschen M, Peled A, Davis RE, Konopleva M, Burger JA.  Effects of pharmacological and genetic disruption of CXCR4 chemokine receptor function in B-cell acute lymphoblastic leukaemia.  Br. J. Haematol.  2016  174:425-36  PubMed ID: 27071778



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