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Cell No. : Cell Name
RCB1934 : HUT78  update : 2024/08/14
CommentHuman cell line derived from T cell of a patient with Sezary syndrome. Producing IL-2. TKG0375 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0375
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue Peripheral blood
Disease name Peripheral blood of a patient with Sezary syndrome
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0375)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0337
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 2
User's Publication 2


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Reference
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
5046  Gootenberg JE, Ruscetti FW, Mier JW, Gazdar A, Gallo RC.  Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.  J Exp Med  1981  154(5):1403-18  PubMed ID: 6975346   DOI: 10.1084/jem.154.5.1403

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User's Publication
13384  Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.  Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.  Oncol Lett  2021    PubMed ID: 33376545   DOI: 10.3892/ol.2020.12374
15877  Kitagawa K, Shibata K, Matsumoto A, Matsumoto M, Ohhata T, Nakayama KI, Niida H, Kitagawa M.  Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development  Mol Cell Biol  2014  34(14):2732-44  PubMed ID: 24820417   DOI: 10.1128/MCB.01549-13



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