Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1934 : HUT78  update : 2021/02/26
CommentHuman cell line derived from T cell of a patient with Sezary syndrome. Producing IL-2. TKG0375 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0375
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue Peripheral blood
Disease name Peripheral blood of a patient with Sezary syndrome
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0375)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Reference information Reference 2
User's Publication 1


To topTop
Reference
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
5046  Gootenberg JE, Ruscetti FW, Mier JW, Gazdar A, Gallo RC.  Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.  J Exp Med  1981  154(5):1403-18  PubMed ID: 6975346

To topTop
User's Publication
13384  Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.  Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.  Oncol Lett  2021    PubMed ID: 33376545



Back Back Return Top Page