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Cell No. : Cell Name
RCB1933 : NALM-6  update : 2025/10/08
CommentHuman cell line derived from B cell leukemia. TKG0413 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0413
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue blood
Disease name B cell of ALL
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University（TKG0413）
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0092
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma/Acholeplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 9
User's Publication 14


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Reference
3527  Rowland AJ, Pietersz GA, McKenzie IF.  Preclinical investigation of the antitumour effects of anti-CD19-idarubicin immunoconjugates.  Cancer Immunol Immunother  1993  37(3):195-202  PubMed ID: 7687521   DOI: 10.1007/bf01525435
5041  Luo Y, Seon BK.  Marked difference in the in vivo antitumor efficacy between two immunotoxins targeted to different epitopes of common acute lymphoblastic leukemia antigen (CD10). Mechanisms involved in the differential activities of immunotoxins.  J Immunol  1990  145(6):1974-82  PubMed ID: 1697314  
5040  Luo Y, Hara H, Haruta Y, Seon BK.  Establishment of ascitic tumor of human pre-B acute lymphoblastic leukemia in nonconditioned nude mice.  Cancer Res  1989  49(3):706-10  PubMed ID: 2783384  
3522  Pulczynski S, Boesen AM, Jensen OM, Ellegaard J, Hokland P.  Immunoelectron microscopy studies on the modulation of common acute lymphoblastic leukemia antigen (CALLA) on NALM-6 cells: delineation of intracellular transport.  Leukemia  1989  3(2):137-44  PubMed ID: 2521368  
3523  Shipp MA, Vijayaraghavan J, Schmidt EV, Masteller EL, D'Adamio L, Hersh LB, Reinherz EL.  Common acute lymphoblastic leukemia antigen (CALLA) is active neutral endopeptidase 24.11 ("enkephalinase"): direct evidence by cDNA transfection analysis.  Proc Natl Acad Sci U S A  1989  86(1):297-301  PubMed ID: 2521388   DOI: 10.1073/pnas.86.1.297
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
5045  Han T, Dadey B, Minowada J.  Cultured human leukemic non-T/non-B lymphoblasts and their stimulating capacity in "one-way" mixed lymphocyte reaction: suggestive evidence for early T-cell or B-cell precursors.  Cancer  1979  44(1):136-40  PubMed ID: 156581   DOI: 10.1002/1097-0142(197907)44:1<136::aid-cncr2820440124>3.0.co;2-i
5043  Han T, Minowada J.  Use of stimulating capacity of mixed lymphocyte reaction (MLR-S) as a possible marker for the cell-origin of null-cell acute lymphoblastic leukaemia.  Immunology  1978  35(2):333-9  PubMed ID: 155647  
5044  Han T, Dadey B, Minowada J.  Unique leukemic non-T/non-B lymphoid cell lines (REH and KM-3): absence of MLR-S and presence of suppressor cell activity for normal T-cell response.  J Clin Lab Immunol  1978  1(3):237-43  PubMed ID: 159361  

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User's Publication
19668  Lu X, Lofgren SM, Zhao Y, Mazur PK.  Multiplexed transcriptomic profiling of the fate of human CAR T cells in vivo via genetic barcoding with shielded small nucleotides  Nat Biomed Eng  2023  7(9):1170-1187  PubMed ID: 37652986   DOI: 10.1038/s41551-023-01085-3
21352  Mise-Omata S, Ando M, Srirat T, Nakagawara K, Hayakawa T, Iizuka-Koga M, Nishimasu H, Nureki O, Ito M, Yoshimura A.  SOCS3 deletion in effector T cells confers an anti-tumorigenic role of IL-6 to the pro-tumorigenic cytokine.  Cell Rep  2023  112940  PubMed ID: 37582370   DOI: 10.1016/j.celrep.2023.112940
20013  Iriguchi S, Yasui Y, Kawai Y, Arima S, Kunitomo M, Sato T, Ueda T, Minagawa A, Mishima Y, Yanagawa N, Baba Y, Miyake Y, Nakayama K, Takiguchi M, Shinohara T, Nakatsura T, Yasukawa M, Kassai Y, Hayashi A, Kaneko S.  A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.  Nat Commun  2021  12(1):430  PubMed ID: 33462228   DOI: 10.1038/s41467-020-20658-3
20353  Min Q, Meng X, Zhou Q, Wang Y, Li Y, Lai N, Xiong E, Wang W, Yasuda S, Yu M, Zhang H, Sun J, Wang X, Wang JY.  RAG1 splicing mutation causes enhanced B cell differentiation and autoantibody production.  JCI Insight  2021  6(19)  PubMed ID: 34622798   DOI: 10.1172/jci.insight.148887
16757  Yamashita M, Kuehn HS, Okuyama K, Okada S, Inoue Y, Mitsuiki N, Imai K, Takagi M, Kanegane H, Takeuchi M, Shimojo N, Tsumura M, Padhi AK, Zhang KYJ, Boisson B, Casanova JL, Ohara O, Rosenzweig SD, Taniuchi I, Morio T.  A variant in human AIOLOS impairs adaptive immunity by interfering with IKAROS  Nat Immunol  2021  22(7):893-903  PubMed ID: 34155405   DOI: 10.1038/s41590-021-00951-z
13842  Hsieh HY, Jia W, Jin ZC, Kidoya H, Takakura N.  High expression of PSF1 promotes drug resistance and cell cycle transit in leukemia cells  Cancer Sci  2020  111(7):2400-2412  PubMed ID: 32391593   DOI: 10.1111/cas.14452
14798  Kondo T, Ando M, Nagai N, Tomisato W, Srirat T, Liu B, Mise-Omata S, Ikeda M, Chikuma S, Nishimasu H, Nureki O, Ohmura M, Hayakawa N, Hishiki T, Uchibori R, Ozawa K, Yoshimura A.  The NOTCH-FOXM1 Axis Plays a Key Role in Mitochondrial Biogenesis in the Induction of Human Stem Cell Memory-like CAR-T Cells  Cancer Res  2020  80(3):471-483  PubMed ID: 31767627   DOI: 10.1158/0008-5472.CAN-19-1196
10269  Komohara Y, Ma C, Yano H, Pan C, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Okuno Y, Nosaka K, Shimosaki S, Morishita K, Matsuoka M, Wakayama T, Takeya M.  Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages  J Clin Exp Hematop  2017  57:15-20  PubMed ID: 28420814   DOI: 10.3960/jslrt.17003
14232  Yasunaga M, Manabe S, Matsumura Y.  Immunoregulation by IL-7R-targeting antibody-drug conjugates: overcoming steroid-resistance in cancer and autoimmune disease  Sci Rep  2017  7(1):10735  PubMed ID: 28878234   DOI: 10.1038/s41598-017-11255-4
18185  Weeks RJ, Ludgate JL, LeMée G, Morison IM.  TESTIN Induces Rapid Death and Suppresses Proliferation in Childhood B Acute Lymphoblastic Leukaemia Cells  PLoS One  2016  11(3):e0151341  PubMed ID: 26985820   DOI: 10.1371/journal.pone.0151341
9722  Richmond J, Robbins A, Evans K, Beck D, Kurmasheva RT, Billups CA, Carol H, Heatley S, Sutton R, Marshall GM, White D, Pimanda J, Houghton PJ, Smith MA, Lock RB.  Acute Sensitivity of Ph-like Acute Lymphoblastic Leukemia to the SMAC-Mimetic Birinapant.  Cancer Res.  2016  76:4579-91  PubMed ID: 27302164   DOI: 10.1158/0008-5472.CAN-16-0523
6423  Yamamoto H, Ishimura M, Ochiai M, Takada H, Kusuhara K, Nakatsu Y, Tsuzuki T, Mitani K, Hara T.  BTK gene targeting by homologous recombination using a helper-dependent adenovirus/adeno-associated virus hybrid vector.  Gene Ther  2016  23(2):205-13  PubMed ID: 26280081   DOI: 10.1038/gt.2015.91
13994  Horlad H, Ma C, Yano H, Pan C, Ohnishi K, Fujiwara Y, Endo S, Kikukawa Y, Okuno Y, Matsuoka M, Takeya M, Komohara Y.  An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma  Cancer Sci  2016  107(11):1696-1704  PubMed ID: 27564404   DOI: 10.1111/cas.13065
6132  Naito H, Kidoya H, Sato Y, Takakura N.  Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.  J Biochem  2009  145(5):653-9  PubMed ID: 19179360   DOI: 10.1093/jb/mvp021


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