Back Back
Cell No. : Cell Name
RCB1933 : NALM-6  update : 2021/02/26
CommentHuman cell line derived from B cell leukemia. TKG0413 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0413
Animal human < Mammals
Genus Homo
Species sapiens
Tissue blood
Disease name B cell of ALL
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0413)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Reference information Reference 9
User's Publication 5

To topTop
3527  Rowland AJ, Pietersz GA, McKenzie IF.  Preclinical investigation of the antitumour effects of anti-CD19-idarubicin immunoconjugates.  Cancer Immunol Immunother  1993  37(3):195-202  PubMed ID: 7687521
5041  Luo Y, Seon BK.  Marked difference in the in vivo antitumor efficacy between two immunotoxins targeted to different epitopes of common acute lymphoblastic leukemia antigen (CD10). Mechanisms involved in the differential activities of immunotoxins.  J Immunol  1990  145(6):1974-82  PubMed ID: 1697314
5040  Luo Y, Hara H, Haruta Y, Seon BK.  Establishment of ascitic tumor of human pre-B acute lymphoblastic leukemia in nonconditioned nude mice.  Cancer Res  1989  49(3):706-10  PubMed ID: 2783384
3522  Pulczynski S, Boesen AM, Jensen OM, Ellegaard J, Hokland P.  Immunoelectron microscopy studies on the modulation of common acute lymphoblastic leukemia antigen (CALLA) on NALM-6 cells: delineation of intracellular transport.  Leukemia  1989  3(2):137-44  PubMed ID: 2521368
3523  Shipp MA, Vijayaraghavan J, Schmidt EV, Masteller EL, D'Adamio L, Hersh LB, Reinherz EL.  Common acute lymphoblastic leukemia antigen (CALLA) is active neutral endopeptidase 24.11 ("enkephalinase"): direct evidence by cDNA transfection analysis.  Proc Natl Acad Sci U S A  1989  86(1):297-301  PubMed ID: 2521388
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
5045  Han T, Dadey B, Minowada J.  Cultured human leukemic non-T/non-B lymphoblasts and their stimulating capacity in "one-way" mixed lymphocyte reaction: suggestive evidence for early T-cell or B-cell precursors.  Cancer  1979  44(1):136-40  PubMed ID: 156581
5043  Han T, Minowada J.  Use of stimulating capacity of mixed lymphocyte reaction (MLR-S) as a possible marker for the cell-origin of null-cell acute lymphoblastic leukaemia.  Immunology  1978  35(2):333-9  PubMed ID: 155647
5044  Han T, Dadey B, Minowada J.  Unique leukemic non-T/non-B lymphoid cell lines (REH and KM-3): absence of MLR-S and presence of suppressor cell activity for normal T-cell response.  J Clin Lab Immunol  1978  1(3):237-43  PubMed ID: 159361

To topTop
User's Publication
20013  Iriguchi S, Yasui Y, Kawai Y, Arima S, Kunitomo M, Sato T, Ueda T, Minagawa A, Mishima Y, Yanagawa N, Baba Y, Miyake Y, Nakayama K, Takiguchi M, Shinohara T, Nakatsura T, Yasukawa M, Kassai Y, Hayashi A, Kaneko S.  A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.  Nat Commun  2021  12(1):430  PubMed ID: 33462228
10269  Komohara Y, Ma C, Yano H, Pan C, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Okuno Y, Nosaka K, Shimosaki S, Morishita K, Matsuoka M, Wakayama T, Takeya M.  Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages.  J Clin Exp Hematop  2017  57:15-20  PubMed ID: 28420814
9722  Richmond J, Robbins A, Evans K, Beck D, Kurmasheva RT, Billups CA, Carol H, Heatley S, Sutton R, Marshall GM, White D, Pimanda J, Houghton PJ, Smith MA, Lock RB.  Acute Sensitivity of Ph-like Acute Lymphoblastic Leukemia to the SMAC-Mimetic Birinapant.  Cancer Res.  2016  76:4579-91  PubMed ID: 27302164
6423  Yamamoto H, Ishimura M, Ochiai M, Takada H, Kusuhara K, Nakatsu Y, Tsuzuki T, Mitani K, Hara T.  BTK gene targeting by homologous recombination using a helper-dependent adenovirus/adeno-associated virus hybrid vector.  Gene Ther  2016  23(2):205-13  PubMed ID: 26280081
6132  Naito H, Kidoya H, Sato Y, Takakura N.  Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.  J Biochem  2009  145(5):653-9  PubMed ID: 19179360

Back Back Return Top Page