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Cell No. : Cell Name
RCB1932 : T2  update : 2022/07/07
CommentHuman hybrid (T and B lymphoblast) cell line. Fusion cll line between 174 and CEM.T2. This hybrid cells do not express MHC class II. HLA-A2 positive. TKG0599 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0599
Animal human x human < Hybrid
Genus Homo
Species sapiens
Tissue hybrid cell
Disease name 174 x CEM.T2
Classification hybrid cells
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0599)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2211
hybridoma Fusion_cell_1 name LCL721 B cell
Fusion_cell_1 strain human
Fusion_cell_2 name CEM.T cell
Fusion_cell_2 strain human
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 81-90(50) : /84(6),85(7),86(8),87(6),88(8),89(5)/
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 6

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3274  Salter, R D, Howell, D N, Cresswell P  Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids.  Immunogenetics  1985  21:235-46  PubMed ID: 3872841   DOI: 10.1007/bf00375376
3281  Kavathas P, Bach, F H, DeMars R  Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.  Proc Natl Acad Sci U S A  1980  77:4251-5  PubMed ID: 6933474   DOI: 10.1073/pnas.77.7.4251

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User's Publication
9576  Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T.  Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate.  Int. J. Oncol.  2016  48:1794-804  PubMed ID: 26936487   DOI: 10.3892/ijo.2016.3403
13717  Tomita Y, Yuno A, Tsukamoto H, Senju S, Yoshimura S, Osawa R, Kuroda Y, Hirayama M, Irie A, Hamada A, Jono H, Yoshida K, Tsunoda T, Kohrogi H, Yoshitake Y, Nakamura Y, Shinohara M, Nishimura Y.  Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients  Int J Cancer  2014  134(2):352-66  PubMed ID: 24734272   DOI: 10.1002/ijc.28376
13704  Nobuoka D, Yoshikawa T, Takahashi M, Iwama T, Horie K, Shimomura M, Suzuki S, Sakemura N, Nakatsugawa M, Sadamori H, Yagi T, Fujiwara T, Nakatsura T.  Intratumoral peptide injection enhances tumor cell antigenicity recognized by cytotoxic T lymphocytes: a potential option for improvement in antigen-specific cancer immunotherapy  Cancer Immunol Immunother  2013  62(4):639-52  PubMed ID: 23143746   DOI: 10.1007/s00262-012-1366-6
7984  Tomita Y, Imai K, Senju S, Irie A, Inoue M, Hayashida Y, Shiraishi K, Mori T, Daigo Y, Tsunoda T, Ito T, Nomori H, Nakamura Y, Kohrogi H, Nishimura Y.  A novel tumor-associated antigen, cell division cycle 45-like can induce cytotoxic T-lymphocytes reactive to tumor cells.  Cancer Sci.  2011  102:697-705  PubMed ID: 21231984   DOI: 10.1111/j.1349-7006.2011.01865.x
12296  Katsunori Imai, Shinya Hirata, Atsushi Irie, Satoru Senju, Yoshiaki Ikuta, Kazunori Yokomine, Michiko Harao, Mitsuhiro Inoue, Takuya Tsunoda, Shuichi Nakatsuru, Hidewaki Nakagawa, Yusuke Nakamura, Hideo Baba, Yasuharu Nishimura  Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers  Clin Cancer Res  2008  14(20):6487-95.  PubMed ID: 18927288   DOI: 10.1158/1078-0432.CCR-08-1086
12297  Michiko Harao, Shinya Hirata, Atsushi Irie, Satoru Senju, Tetsuya Nakatsura, Hiroyuki Komori, Yoshiaki Ikuta, Kazunori Yokomine, Katsunori Imai, Mitsuhiro Inoue, Kumiko Harada, Takeshi Mori, Takuya Tsunoda, Shuichi Nakatsuru, Yataro Daigo, Hiroaki Nomori, Yusuke Nakamura, Hideo Baba, Yasuharu Nishimura  HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL  Int J Cancer  2008  123(11):2616-25  PubMed ID: 18770861   DOI: 10.1002/ijc.23823

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