Cell No. : Cell Name
RCB1932 : T2
update : 2024/08/14
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| Comment | Human hybrid (T and B lymphoblast) cell line. Fusion cll line between 174 and CEM.T2. This hybrid cells do not express MHC class II. HLA-A2 positive. TKG0599 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Obinata, Masuo
|
| Year of deposit |
2004
|
| Original cell |
TKG0599
|
| Animal |
_human x human
< Hybrid
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
hybrid cell
|
| Disease name |
174 x CEM.T2
|
| Classification |
hybrid cells
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0599）
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_2211
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hybridoma
|
Fusion_cell_1 name |
LCL721 B cell
|
| Fusion_cell_1 strain |
human
|
| Fusion_cell_2 name |
CEM.T cell
|
| Fusion_cell_2 strain |
human
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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LT 400
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| Virus (HTLV-1) |
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(-)
|
| Isozyme |
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LD, NP
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| Chromosome mode |
|
81-90(50) : /84(6),85(7),86(8),87(6),88(8),89(5)/
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| STR(human) |
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OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
6
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| User's Publication |
|
9576
Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T.
Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate.
Int. J. Oncol.
2016
48:1794-804
PubMed ID: 26936487
DOI: 10.3892/ijo.2016.3403
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13717
Tomita Y, Yuno A, Tsukamoto H, Senju S, Yoshimura S, Osawa R, Kuroda Y, Hirayama M, Irie A, Hamada A, Jono H, Yoshida K, Tsunoda T, Kohrogi H, Yoshitake Y, Nakamura Y, Shinohara M, Nishimura Y.
Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients
Int J Cancer
2014
134(2):352-66
PubMed ID: 24734272
DOI: 10.1002/ijc.28376
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13704
Nobuoka D, Yoshikawa T, Takahashi M, Iwama T, Horie K, Shimomura M, Suzuki S, Sakemura N, Nakatsugawa M, Sadamori H, Yagi T, Fujiwara T, Nakatsura T.
Intratumoral peptide injection enhances tumor cell antigenicity recognized by cytotoxic T lymphocytes: a potential option for improvement in antigen-specific cancer immunotherapy
Cancer Immunol Immunother
2013
62(4):639-52
PubMed ID: 23143746
DOI: 10.1007/s00262-012-1366-6
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7984
Tomita Y, Imai K, Senju S, Irie A, Inoue M, Hayashida Y, Shiraishi K, Mori T, Daigo Y, Tsunoda T, Ito T, Nomori H, Nakamura Y, Kohrogi H, Nishimura Y.
A novel tumor-associated antigen, cell division cycle 45-like can induce cytotoxic T-lymphocytes reactive to tumor cells.
Cancer Sci.
2011
102:697-705
PubMed ID: 21231984
DOI: 10.1111/j.1349-7006.2011.01865.x
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12296
Katsunori Imai, Shinya Hirata, Atsushi Irie, Satoru Senju, Yoshiaki Ikuta, Kazunori Yokomine, Michiko Harao, Mitsuhiro Inoue, Takuya Tsunoda, Shuichi Nakatsuru, Hidewaki Nakagawa, Yusuke Nakamura, Hideo Baba, Yasuharu Nishimura
Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers
Clin Cancer Res
2008
14(20):6487-95.
PubMed ID: 18927288
DOI: 10.1158/1078-0432.CCR-08-1086
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12297
Michiko Harao, Shinya Hirata, Atsushi Irie, Satoru Senju, Tetsuya Nakatsura, Hiroyuki Komori, Yoshiaki Ikuta, Kazunori Yokomine, Katsunori Imai, Mitsuhiro Inoue, Kumiko Harada, Takeshi Mori, Takuya Tsunoda, Shuichi Nakatsuru, Yataro Daigo, Hiroaki Nomori, Yusuke Nakamura, Hideo Baba, Yasuharu Nishimura
HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL
Int J Cancer
2008
123(11):2616-25
PubMed ID: 18770861
DOI: 10.1002/ijc.23823
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