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Cell No. : Cell Name
RCB1928 : KG-1a  update : 2021/02/26
CommentHuman cell line derived from acute myelogenous leukemia. The variant subline of KG-1(RCB1166) cell line. TKG0402 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Koeffler,H.P. & Golde,D,W.
Year of deposit 2004
Original cell TKG0402
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 59 years
Tissue bone marrow
Disease name acute myelogenous leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0402)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 2


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Reference
880  Koeffler, H P  Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications.  Blood  1983  62:709-21  PubMed ID: 6192859
1937  Koeffler, H P, Golde, D W  Human myeloid leukemia cell lines: a review.  Blood  1980  56:344-50  PubMed ID: 6996765
625  Koeffler, H P, Golde, D W  Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity.  Science  1978  200:1153-4  PubMed ID: 306682

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User's Publication
13354  Noura M, Morita K, Kiyose H, Matsuo H, Nishinaka-Arai Y, Kurokawa M, Kamikubo Y, Adachi S.  Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells.  Sci Rep  2020    PubMed ID: 33219287
6132  Naito H, Kidoya H, Sato Y, Takakura N.  Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.  J Biochem  2009  145(5):653-9  PubMed ID: 19179360



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