| Cell No. : Cell Name RCB1928 : KG-1a
						
						 update : 2025/05/21
 | 
						
						
						
							
								| Comment | Human cell line derived from acute myelogenous leukemia. The variant subline of KG-1(RCB1166) cell line.   TKG0402   (Deposited from Tohoku Univ.). | 
							
								| Comment from the depositor |  | 
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
								| Remarks |  | 
							
							
								| Order Form | Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										  | 
							
								| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | 
						
						
					
						
						
							
							|  | Basic information | Depositor | Obinata, Masuo | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Originator | Koeffler,H.P. & Golde,D,W. | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of deposit | 2004 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Original cell | TKG0402 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Animal | _human
								
								
								
							
								 
								
								
								 	
								 		< Mammals | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Genus | Homo | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Species | sapiens | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Gender | Male | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Age at sampling | 59 years | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Tissue | bone marrow | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Disease name | acute myelogenous leukemia | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Classification | cancer | 
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | History | Cell Resource Center for Biomedical Research, Tohoku University(TKG0402) | 
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							 | Lifespan | infinite | 
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | lymphocyte-like | 
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Cellosaurus(Expasy) | CVCL_1824 | 
					
									
								
							
						
					
					
					
					
					
					
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
					
						
						
							
							
								|  |  | deposit info | lot info | 
											
												|  | Medium | Medium List | 
										
									
								
						
						
						
						
						
					
						
						
						
						
							 | Culture type |  | Suspension cells | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Culture method |  | General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese) | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Culture medium |  | RPMI1640 + 10% FBS | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Antibiotics |  | Free | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							Culture information | Passage ratio |  | 1 : 8 split | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| SC frequency |  | Subculture : 2 times/week | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Temperature |  | 37
							
							
						
						
							℃ | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| CO2 concentration |  | 5
							
							
						
						
							% | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freeze medium |  | Medium + 10% DMSO | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freezing method |  | Slow freezing | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Mycoplasma/Acholeplasma |  | (-) | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Virus (HIV) |  | Undetected | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Virus (HTLV-1) |  | (-) | 
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Isozyme |  | LD, NP | 
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| STR(human) |  | OK | 
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
			
					
			
						
						
						
							
								| Reference information | Reference | 4 | 
							
								| User's Publication | 13 | 
						
						
				
			
				
				
				
				
				
					
						| User's Publication | 
						
							
							| 22956 
								Li J, Wang Z, Qin X, Zhong MC, Tang Z, Qian J, Dou J, Hussell T, King PD, Nunès JA, Yamanashi Y, Davidson D, Veillette A. 
								CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth. 
								Nat Commun 
								2025 
								16(1):5145 
								
									
										PubMed ID: 40461553
									
								 
								
									
										DOI: 10.1038/s41467-025-60456-3 | 
						
							
							| 19941 
								Dong X, Xue S, Chen C, Jiang Z, Wu X, Wang W. 
								MYCT-1 Gene Expression in Patients with Gastric Cancer: an Ex Vivo Study 
								Appl Biochem Biotechnol 
								2024 
								196(8):5114-5126 
								
									
										PubMed ID: 38112991
									
								 
								
									
										DOI: 10.1007/s12010-023-04754-w | 
						
							
							| 21650 
								Matsuo H, Inagami A, Ito Y, Ito N, Iyoda S, Harata Y, Higashitani M, Shoji K, Tanaka M, Noura M, Mikami T, Kato I, Takita J, Nakahata T, Adachi S. 
								Parbendazole as a promising drug for inducing differentiation of acute myeloid leukemia cells with various subtypes. 
								Commun Biol 
								2024 
								7(1):123 
								
									
										PubMed ID: 38267545
									
								 
								
									
										DOI: 10.1038/s42003-024-05811-8 | 
						
							
							| 22342 
								Shunko A. Inada 
								Biochips to measure ultraviolet light absorption by leukemia cells 
								Japanese Journal of Applied Physics 
								2024 
								63:026503 
								 
								
									
										DOI: 10.35848/1347-4065/ad1e85 | 
						
							
							| 22527 
								Chueahongthong F, Chiampanichayakul S, Viriyaadhammaa N, Dejkriengkraikul P, Okonogi S, Berkland C, Anuchapreeda S. 
								Cytotoxicity of Doxorubicin-Curcumin Nanoparticles Conjugated with Two Different Peptides (CKR and EVQ) against FLT3 Protein in Leukemic Stem Cells 
								Polymers (Basel) 
								2024 
								16(17):2498 
								
									
										PubMed ID: 39274131
									
								 
								
									
										DOI: 10.3390/polym16172498 | 
						
							
							| 21364 
								Yamanaka S, Furihata H, Yanagihara Y, Taya A, Nagasaka T, Usui M, Nagaoka K, Shoya Y, Nishino K, Yoshida S, Kosako H, Tanokura M, Miyakawa T, Imai Y, Shibata N, Sawasaki T. 
								Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation. 
								Nat Commun 
								2023 
								14(1):4683 
								
									
										PubMed ID: 37596276
									
								 
								
									
										DOI: 10.1038/s41467-023-40385-9 | 
						
							
							| 16840 
								Sameh A. Mohammed, Yasuhiro Kimura, Yuhki Toku, Yang Ju 
								Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia 
								Biomaterials and Biosystems 
								2022 
								6:100045 
								 
								
									
										DOI: 10.1016/j.bbiosy.2022.100045 | 
						
							
							| 20347 
								Katayama K, Nishihata A. 
								RSK inhibition induces apoptosis by downregulating protein synthesis in a variety of acute myeloid leukemia cell lines. 
								Biol Pharm Bull 
								2021 
								 
								
									
										PubMed ID: 34602526
									
								 
								
									
										DOI: 10.1248/bpb.b21-00531 | 
						
							
							| 20437 
								Mahara A, Kitagawa K, Otaka A, Nakaoki T, Ishihara K, Yamaoka T. 
								Impact of REDV peptide density and its linker structure on the capture, movement, and adhesion of flowing endothelial progenitor cells in microfluidic devices. 
								Mater Sci Eng C Mater Biol Appl 
								2021 
								129:112381 
								
									
										PubMed ID: 34579900
									
								 
								
									
										DOI: 10.1016/j.msec.2021.112381 | 
						
							
							| 16209 
								Takeshi Ueda, Akinori Kanai, Akiyoshi Komuro, Hisayuki Amano, Kazushige Ota, Masahiko Honda, Masahito Kawazu, Hitoshi Okada 
								KDM4B promotes acute myeloid leukemia associated with AML1-ETO by regulating chromatin accessibility 
								FASEB Bioadv  
								2021 
								3(12):1020-1033 
								
									
										PubMed ID: 34938963
									
								 
								
									
										DOI: 10.1096/fba.2021-00030 | 
						
							
							| 13354 
								Noura M, Morita K, Kiyose H, Matsuo H, Nishinaka-Arai Y, Kurokawa M, Kamikubo Y, Adachi S. 
								Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. 
								Sci Rep 
								2020 
								 
								
									
										PubMed ID: 33219287
									
								 
								
									
										DOI: 10.1038/s41598-020-76951-0 | 
						
							
							| 13842 
								Hsieh HY, Jia W, Jin ZC, Kidoya H, Takakura N. 
								High expression of PSF1 promotes drug resistance and cell cycle transit in leukemia cells 
								Cancer Sci 
								2020 
								111(7):2400-2412 
								
									
										PubMed ID: 32391593
									
								 
								
									
										DOI: 10.1111/cas.14452 | 
						
							
							| 6132 
								Naito H, Kidoya H, Sato Y, Takakura N. 
								Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population. 
								J Biochem 
								2009 
								145(5):653-9 
								
									
										PubMed ID: 19179360
									
								 
								
									
										DOI: 10.1093/jb/mvp021 |