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Cell No. : Cell Name
RCB1926 : OT11  update : 2023/01/05
CommentRBP-J deficient mouse fibroblasts
Comment from the depositor
Terms and conditions1) In relation to the use of for-profit institution, a prior written permission of the DEPOSITOR is required. In relation to the use of not-for-profit institution, a prior written permission of the DEPOSITOR is not required. 2) The RECIPENT agrees to use the BIOLOGICAL RESOURCE only for academic research purpose. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 1997;124:4133-4141) designated by the DEPOSITOR is required. 4) Any patenting action by use of the BIOLOGICAL RESORCE requires a prior consent of the DEPOSITOR. 5) Transfer of the BIOLOGICAL REOSRCE to the third party from the RECIPIENT is prohibited without a prior permission of the DEPOSITOR.
approver's address
Graduate School of Medicine, Kyoto University

Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501 Japan
Dr.HONJO Tasuku
Fax. +81-75-753-4388
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Honjo, Tasuku
Originator Honjo, Tasuku
Year of deposit 2004
Animal _mouse < Mammals
Genus Mus
Species musculus
Age at sampling 9.5 days embryo
Tissue embryo
Classification mutant
Recombinant recombinant
Exogene H-2Kb-tsA58
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T370
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 10% FBS + 100U/ml IFN-γ
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 3

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2800  Kato H, Taniguchi Y, Kurooka H, Minoguchi S, Sakai T, Nomura-Okazaki S, Tamura K, Honjo T  Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives.  Development  1997  124:4133-41  PubMed ID: 9374409   DOI: 10.1242/dev.124.20.4133

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User's Publication
4017  Chang PJ, Wang SS, Chen LY, Hung CH, Huang HY, Shih YJ, Yen JB, Liou JY, Chen LW.  ORF50-dependent and ORF50-independent activation of the ORF45 gene of Kaposi's sarcoma-associated herpesvirus.  Virology  2013  442:38-50  PubMed ID: 23601787   DOI: 10.1016/j.virol.2013.03.023
4016  Wang SS, Chang PJ, Chen LW, Chen LY, Hung CH, Liou JY, Yen JB.  Positive and negative regulation in the promoter of the ORF46 gene of Kaposi's sarcoma-associated herpesvirus.  Virus Res.  2012  165:157-69  PubMed ID: 22366521   DOI: 10.1016/j.virusres.2012.02.010
7843  Li H, Solomon E, Duhachek Muggy S, Sun D, Zolkiewska A.  Metalloprotease-disintegrin ADAM12 expression is regulated by Notch signaling via microRNA-29.  J. Biol. Chem.  2011  286:21500-10  PubMed ID: 21518768   DOI: 10.1074/jbc.M110.207951

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