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Cell No. : Cell Name
RCB1925 : WA-mFib  update : 2023/01/05
CommentMouse stromal cell line for human lung small cell carcinoma cell line, WA-ht.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takiguchi, Yuichi
Year of deposit 2004
Animal _mouse < Mammals
Strain name BALB/c(nu/nu)
Gender Unknown
Tissue skin
Classification other
Year of origin 1990
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U894
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 2

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3487  Sugimoto T, Takiguchi Y, Kurosu K, Kasahara Y, Tanabe N, Tatsumi K, Hiroshima K, Minamihisamatsu M, Miyamoto T, Kuriyama T.  Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer.  Oncol Rep  2005  14(4):823-30  PubMed ID: 16142338  

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User's Publication
20352  Takenaga K, Koshikawa N, Nagase H.  Intercellular transfer of mitochondrial DNA carrying metastasis-enhancing pathogenic mutations from high- to low-metastatic tumor cells and stromal cells via extracellular vesicles.  BMC Mol Cell Biol  2021  22(1):52  PubMed ID: 34615464   DOI: 10.1186/s12860-021-00391-5
7193  Yamazoe H, Hagihara Y, Kobayashi H.  Multicomponent Coculture System of Cancer Cells and Two Types of Stromal Cells for In Vitro Evaluation of Anticancer Drugs.  Tissue Eng Part C Methods  2016  22:20-9  PubMed ID: 26421875   DOI: 10.1089/ten.TEC.2015.0188

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