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Cell No. : Cell Name
RCB1923 : 13D10  update : 2022/05/23
CommentHybridoma producing antibody against the purified basal apparatus of sperm of the an-1 strain of fern Lygodium.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cytologia 2003;68:271-281) designated by the DEPOSITOR is required.
Remarks
approver's address
×
English
Address
University of Tsukuba

1-1-1, Tenno-dai, Tsukuba, Ibaraki, 305-8577 Japan
Dr.MIYAMURA Shinichi
Fax. +81-29-853-6614
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyamura, Shinichi
Originator Sakaushi, Shinji
Year of deposit 2004
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1996
History Sakaushi, Shinji (Osaka City Univ.) -> Miyamura, Shinichi
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T729
hybridoma Fusion_cell_1 name P3-X63-Ag8.653
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG 1500
Antibody name 13D10
Subclass IgG1
Immunogen sperm of the an-1 strain of fern Lygodium japonicum by detergent extraction
Antigen_name p43
Antigen_m.w. 43kDa
Antigen_character flagellar root (spline), MLS, crest, basal bodies
Antigen_epitope basal bodies
Antigen_positive basal bodies
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives GIT + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Reference information Reference 1
User's Publication 0


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Reference
3125  Sakaushi, S., Mizutani, T., Miyamura, S. and Hori, T.  Isolation of the MLS-type Flagellar Apparatus from the an-1 mutant of Fern Lygodium japonicum and Its Preliminary Immunological Analysis  Cytologia  2003  68:271-281    DOI: 10.1508/cytologia.68.271

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