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Cell No. : Cell Name
RCB1923 : 13D10 update : 2024/01/17 |
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| Comment | Hybridoma producing antibody against the purified basal apparatus of sperm of the an-1 strain of fern Lygodium. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) A permission of the approver is required. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cytologia 2003;68:271-281) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Miyamura, Shinichi | ||
| Originator | Sakaushi, Shinji | |||
| Year of deposit | 2004 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | |||
| Year of origin | 1996 | |||
| History | Sakaushi, Shinji (Osaka City Univ.) -> Miyamura, Shinichi | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_T729 | hybridoma | Fusion_cell_1 name | P3-X63-Ag8.653 |
| Fusion_cell_1 strain | mouse | |||
| Fusion_cell_2 name | spleen | |||
| Fusion_cell_2 strain | mouse, BALB/c | Fusion method | PEG 1500 | Antibody name | 13D10 |
| Subclass | IgG1 | |||
| Immunogen | sperm of the an-1 strain of fern Lygodium japonicum by detergent extraction | Antigen_name | p43 | |
| Antigen_m.w. | 43kDa | |||
| Antigen_character | flagellar root (spline), MLS, crest, basal bodies | |||
| Antigen_epitope | basal bodies | Antigen_positive | basal bodies | |
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | GIT + 10% FBS | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgG1 | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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